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EMAGE:31355

Prss8 ( MGI:1923810)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355
euxassay_006672_01 euxassay_006672_02 euxassay_006672_03 euxassay_006672_04 euxassay_006672_05
EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355
euxassay_006672_06 euxassay_006672_07 euxassay_006672_08 euxassay_006672_09 euxassay_006672_10
EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355
euxassay_006672_11 euxassay_006672_12 euxassay_006672_13 euxassay_006672_14 euxassay_006672_15
EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355
euxassay_006672_16 euxassay_006672_17 euxassay_006672_18 euxassay_006672_19 euxassay_006672_20
EMAGE:31355 EMAGE:31355 EMAGE:31355 EMAGE:31355
euxassay_006672_21 euxassay_006672_22 euxassay_006672_23 euxassay_006672_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
right lung
strong strong
regionalstrong expression: see section 15 16 17 18 19 20 21 22 23 24
stomach
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 08 09 10 11
kidney calyx
strong strong
regionalstrong expression: see section 08 09 10 11 12 18 19 20 21 22
rectum
moderate moderate
regionalmoderate expression: see section 15
male reproductive system
weak weak
regionalweak expression: see section 14 15
urethra of male
weak weak
regionalweak expression: see section 14 15
cochlear duct
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 18 19 20 21 22 23 24
left lung
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14
cochlea
strong strong
regionalstrong expression: see section 05
midgut
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20
thyroid gland
strong strong
regionalstrong expression: see section 13 14 17 18 moderate expression: see section 12
bladder
moderate moderate
regionalmoderate expression: see section 14 15
esophagus
moderate moderate
regionalmoderate expression: see section 14 15
pancreas
moderate moderate
regionalmoderate expression: see section 10 11 12 13 16 17 18
adenohypophysis
weak weak
regionalweak expression: see section 13 14 15 16 17
submandibular gland primordium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 18 19 20 21
trachea
weak weak
regionalweak expression: see section 15 16
nasal cavity respiratory epithelium
strong strong
regionalstrong expression: see section 17 moderate expression: see section 12 13 16
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 09 17 18 19 20 moderate expression: see section 10 11 12 13 16
hindgut
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T6658
Entity Detected:Prss8, ( MGI:1923810)
Sequence:sense strand is shown

>T6658
GTCCACGCTGGTCACTGCAGAGAGACTAAAGGTGTGTCCCGTCTTCCCCCTGGCCATTTTCTGCCGAGAA
GCCTGCTTCACCTTCCCAGCCTAGATCCTGGGATTCCTCACTCTGATCCCACACCTAGATCCTGCCTTCA
AAACCAGCCTTCTGGTTCTTCCCTTTCTACCTAGGGGCTCCTTCGGGGGGCCCTTGTCCTAGGCCATGGC
CCTAAGGGTGGGCCTGGGACTTGGGCAGCTGGAAGCTGTGACCATTCTGCTCCTTCTCGGATTGCTCCAG
TCGGGAATCCGAGCTGACGGGACTGAAGCCTCCTGTGGTGCCGTCATCCAGCCACGCATCACCGGTGGTG
GCAGTGCAAAGCCCGGTCAGTGGCCCTGGCAGGTCAGCATCACCTACGATGGCAACCATGTTTGTGGCGG
GTCGCTCGTGTCAAATAAATGGGTGGTGTCTGCTGCTCACTGCTTCCCCAGAGAACACAGCAGGGAAGCG
TATGAGGTGAAGCTGGGGGCCCACCAGCTAGACTCCTACAGCAATGACACTGTGGTCCACACAGTGGCTC
AGATCATCACCCACTCAAGCTACCGAGAAGAGGGCTCCCAGGGGGACATCGCGCTCATCCGCCTCAGCAG
TCCTGTCACCTTCTCCCGCTACATCAGACCCATCTGCCTCCCTGCAGCCAATGCCTCCTTTCCCAACGGC
CTTCACTGTACTGTCACGGGATGGGGTCATGTGGCTCCTTCAGTGAGCCTCCAGACCCCTAGGCCTCTGC
AGCAGCTCGAGGTACCACTCATCAGCCGGGAAACCTGTAGCTGCCTGTACAACATTAATGCGGTGCCTGA
AGAACCGCACACTATCCAGCAGGACATGCTGTGTGCTGGCTATGTGAAGGGAGGCAAGGATGCCTGCCAG
GGTGACTCTGGGGGCCCACTCTCTTGTCCCATGGAGGGCATCTGGTACTTGGCAGGCATTGTGAGTTGGG
GTGATGCCTGTGGTGCCCCCAACAGGCCTGGAGTATACACTCTGACTTCTACCTATGCTTCCTGGATCCA
CCACCATGTGGCAGAGCTCCAGCCTCGAGTTGTCCCCCAGACTCAGGAGTCCCAGCCGGATGGCCACCTC
TGCAACCATCATCCTGTCTTCAGCTCAGCGGCAGCCCCGAAATTGTTAAGGCCCGTACTTTTCCTGCCAC
TTGGTCTGACCCTGGGCCTCTTGTCTTTGGCTTGAACATTAAGCTATTTAACCCTCAAAGTTGACCATGC
TTCCAGGACCACAGCACACCCAAGGACATGTGCCTGTCCGGGAGGGCAGCATGCCGACTTGATCTTTGAG
CCCACTCGTCTGCATTTGTTCTTTGGGATTATATAATCTAGGGCCAAGAAGTTTACTGCCTGTGGAGCGG
GCAGGAACTCTAGCCACCTTTCTCTGTTACCTGCAGCTGATTGGCAAGGCTCCTATGGAGCTCCCAGAAG
CCTCAGCTAGGAAAATGAGCCCTCACTTCAGCCTGTATCTAGCACGTGGGGCAGCTGCCTAGCTGACTGC
TGCACAAGGCTGACTCTGGGCTCTACCCACCCTGCCTGATGAGCCCTTCACTTTGTCCTCTTCCTGCCTC
CTGGGCTCTGCCTTAATCTCCTGGACTCCGGAGGACTGAGCCCCCACCGGAGCTGGCTGGGGCTTGGGTC
AGGGTGAGGAATAAAAGGAGCGAGGAATCAATGTTTTTAACACAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3600399 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3600399 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_006672 same experiment
 EMAGE:31905 same embryo
 EMAGE:31908 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS