EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31364

Chga chromogranin A ( MGI:88394)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002048_01	euxassay_002048_02	euxassay_002048_03	euxassay_002048_04	euxassay_002048_05

euxassay_002048_06	euxassay_002048_07	euxassay_002048_08	euxassay_002048_09	euxassay_002048_10

euxassay_002048_11	euxassay_002048_12	euxassay_002048_13	euxassay_002048_14	euxassay_002048_15

euxassay_002048_16	euxassay_002048_17	euxassay_002048_18	euxassay_002048_19	euxassay_002048_20

euxassay_002048_21	euxassay_002048_22	euxassay_002048_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 02 03 04 05 06 09 10 11 12 13 14

facial vii ganglion

strong

regional

strong expression: see section 02 03 05 16 17 18

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 14 15 16 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 11 12 14 15 16 17 weak expression: see section 08 09 10 18 19

cervical ganglion

strong

regional

strong expression: see section 03 04 11

pituitary gland

strong

regional

strong expression: see section 09 10 11 weak expression: see section 08

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 05 13 14 15 weak expression: see section 02 03 04

cervico-thoracic ganglion

strong

regional

strong expression: see section 03 08 09 10

spinal cord

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 13 14 weak expression: see section 02 03

thoracic ganglion

strong

regional

strong expression: see section 08 09 10

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4613
Entity Detected:	Chga, chromogranin A ( MGI:88394)
Sequence:	sense strand is shown >T4613 CCCACGCGTCCGACCGCTGTTACCACCACCGCTACTGCAGTGTTCCCGCTGGTGCAGAGCTTTGGTAGCC AGACTACAGACCCACTCCCGCCATCCTCCTGCAGCAGCTCGTCCACTCTTTCCGCACCGTCCGGCTCGCT ATGCGCTCCACCGCGGTTCTGGCGCTTCTGCTGTGCGCCGGGCAAGTTTTTGCCCTTCCTGTGAACAGCC CCATGACAAAAGGGGACACCAAGGTGATGAAGTGCGTCCTGGAAGTCATCTCCGACTCGCTGTCCAAACC CAGCCCCATGCCTGTCAGCCCTGAGTGTCTGGAGACCCTCCAAGGAGACGAGAGGATCCTCTCTATCCTG CGACACCAGAATCTGCTGAAGGAACTTCAAGACCTGGCTCTCCAAGGTGCCAAGGAGCGGGCCCAGCAGC CGCTGAAGCAGCAGCAGCCGCCGAAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGCAGGAGCAGCAACA CAGCAGCTTTGAGGATGAACTCTCAGAAGTGTTTGAGAACCAGAGCCCTGATGCCAAGCACAGAGACGCA GCAGCAGAAGTGCCCTCCAGGGACACTATGGAGAAGAGAAAGGATTCTGACAAGGGACAACAGGATGGCT TTGAGGCAACCACAGAGGGACCTAGGCCTCAGGCCTTTCCAGAGCCTAACCAAGAGTCCCCCATGATGGG AGACAGTGAGTCCCCTGGGGAGGACACAGCCACCAATACCCAATCACCAACCAGCCTCCCCAGCCAGGAG CATGTGGACCCACAGGCCACGGGAGACAGTGAGAGAGGCCTGAGTGCCCAACAGCAAGCCAGAAAAGCCA AGCAAGAGGAGAAAGAAGAGGAGGAGGAAGAGGAGGCTGTGGCTAGAGAGAAGGCTGGGCCTGAAGAAGT CCCCACTGCAGCATCCAGTTCCCACTTCCATGCAGGCTACAAAGCGATCCAGAAAGATGATGGTCAGTCG GACTCTCAGGCAGTGGATGGAGATGGAAAGACAGAGGCTTCCGAAGCTCTGCCGTCTGAAGGGAAGGGGG AGCTGGAGCACTCTCAGCAGGAGGAAGATGGGGAAGAGGCCATGGTAGGGACCCCCCAAGGTCTCTTCCC CCAAGGCGGGAAGGGCCGGGAGCTGGAACATAAGCAGGAGGAAGAGGAAGAGGAGGAGGAGCGTCTGTCC AGAGAATGGGAGGACAAGCGATGGAGCAGGATGGACCAGCTGGCCAAAGAGCTGACAGCAGAGAAGCGGC TGGAGGGCGAGGATGACCCTGACCGCTCCATGAAGCTCTCCTTCCGGACCCGCGCCTATGGCTTCAGAGA TCCTGGGCCTCAGCTACGCAGGGGCTGGAGGCCATCTTCCAGAGAAGACAGTGTGGAGGCCCGAAGTGAC TTTGAGGAAAAGAAGGAAGAGGAGGGCAGCGCCAACCGCAGAGCAGAGGACCAGGAGCTAGAGAGCCTGT CAGCCATCGAGGCAGAGCTGGAGAAGGTGGCCCACCAGCTGCAGGCTTTGCGGCGGGGATGAGGCCCTGG CTGGTGGGGCCAGCCATGGCTTCAAGGCCAGACTGCTGCTCGAGTAGGGCTGCTTCCAGCCACAGAGCCC AGGTTACCCCCTTTGCCCCTCACCTTCTCTTGTTCTCAGCCCCTGCCCTGGACACTTCTGCAGGGCAGCC CTGAATGTCAGCACAGATTCCTTCTCTGAACACAGGCAGCTTTCTAGAAGTTTCTCTTCCACCTTCTTAT CCATGGGGCACAACTGCAATAACTTCTGACCTTTGGGCGAAAGCCGAGAACTCCTGACTATTAAGATATT CCAGATAAAATATATTGAAGGAAGAATAAAGTGCTTTGGGCTCTTAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4990941 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4990941 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002048 same experiment
	EMAGE:29490 same embryo
	EMAGE:31391 same embryo
	EMAGE:31374 same embryo
	EMAGE:31397 same embryo
	EMAGE:29503 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source