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EMAGE:31391

Col9a3 ( MGI:894686)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391
euxassay_002050_01 euxassay_002050_02 euxassay_002050_03 euxassay_002050_04 euxassay_002050_05
EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391
euxassay_002050_06 euxassay_002050_07 euxassay_002050_08 euxassay_002050_09 euxassay_002050_10
EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391
euxassay_002050_11 euxassay_002050_12 euxassay_002050_13 euxassay_002050_14 euxassay_002050_15
EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391 EMAGE:31391
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EMAGE:31391 EMAGE:31391 EMAGE:31391
euxassay_002050_21 euxassay_002050_22 euxassay_002050_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
hindlimb digit 5 mesenchyme
strong strong
regionalstrong expression: see section 06 07 08 09 19 20
hindlimb digit 1 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 08 15 16 17 18 19 21
temporal bone
strong strong
regionalstrong expression: see section 12 13 14 15 16 17 18 19 20 21
hindlimb digit 4 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 17 18 19 20
rib
strong strong
regionalstrong expression: see section 18 19 20 21 22
leg
strong strong
regionalstrong expression: see section 01 02 03 04 22 23
axial skeleton
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15
4th ventricle lateral recess
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 10 11 12 13 14
testis
strong strong
regionalstrong expression: see section 02 03 04 05 15 16 17 18
viscerocranium
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 17 18 19
hindlimb digit 2 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 15 16 17 18 19 20 21
basioccipital bone
strong strong
regionalstrong expression: see section 11 15 16 17 19 20
submandibular gland primordium
strong strong
regionalstrong expression: see section 02 03 04 05 11 12 13 14
trachea
strong strong
regionalstrong expression: see section 01 06
basisphenoid bone
strong strong
regionalstrong expression: see section 08 09 10 11 15 16 17 18 19 20
hand mesenchyme
strong strong
regionalstrong expression: see section 01 02 03 04 20 21 22 23
hindlimb digit 3 mesenchyme
strong strong
regionalstrong expression: see section 05 06 07 15 16 17 18 19 20
pectoral girdle and thoracic body wall musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 12 13 15 16 17 18
exoccipital bone
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
orbito-sphenoid
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 10 11 12 13 15 17 18 19 20 21
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4617
Entity Detected:Col9a3, ( MGI:894686)
Sequence:sense strand is shown

>T4617
GGGCAGGCCTGGTCCCAAAGGAATCCCTGGAATGGCTGGGCCAGGCGGAGAACCAGGCATGCCAGGCAAG
GATGGCAAAGATGGTGTGCCGGGACTTGATGGTGAGAAGGGAGAGGCTGGTCGCAATGGTGGCCAAGGAG
AGAAAGGCCCCAATGGGCTGCCGGGGCTCCCTGGACGAGCAGGGTCCAAAGGCGAGAAGGGAGAACCGGG
TAGAACTGGCGAGCTGGGTGAGGCTGGCCCCTCTGGAGAGCCAGGTATCCCTGGAGATGTTGGTGTTCCA
GGGGAGCGTGGTGAGGCTGGTCACAGGGGCTCCGTGGGAGCTCTTGGCCCACAAGGTCCTCCTGGTGCTC
CTGGCATCCGTGGCTTTCAGGGACAAAAAGGCAGCACAGGAGACCCTGGCCTTCCGGGCCCCCAAGGCCT
ACGGGGAGATGTGGGTGACCGGGGTCCAGGAGGTGCCACAGGCCCTAAGGGAGACCAGGGCATTGCAGGT
TCCGATGGTCTTCCAGGGGACAAAGGCGAGCTGGGTCCTAATGGCCCCGTTGGACAAAAAGGAGAGTCTG
GCAGCCGAGGGGAACTGGGCCCCAAAGGCATCCAGGGCCCCAATGGCACCAGTGGAGTCCAGGGTGTACC
TGGTCCCCCAGGTCCACTGGGCCTCCAAGGTGTGCAGGGTGTCCCAGGCATCACCGGGAAGCCTGGAGTT
CCGGGCAAGGAAGCCAGTGAACAGCGCATCAGGGAGCTATGCGGGGGTATGATCAGTGAGCAAATTGCAC
AGTTGGCTGCACACCTGAGGAAGCCCTTAGCACCAGGCTCCATCGGCAGGCCTGGTCCAGCTGGCCCCCC
AGGCCCTCCAGGCCCTCCAGGCTCTATTGGCCACCCTGGCGCTCGGGGTCCCCCTGGATACCGTGGTCCC
ACTGGGGAACTAGGAGATCCTGGACCAAGAGGGAGCCAGGGTGACAGAGGGGACAAGGGAGCAACAGGTG
CAGGGCTGGATGGGCCTGCTGGGGACCAGGGCTACCAAGGGCCTCAAGGTGTACCTGGCATCAGCAAAGA
TGGTCGAGATGGTGCTCATGGTGAGCCTGGCCTTCCCGGTGATCCTGGCCTTCCTGGAGCTGCTGGTGCT
CAGGGTACCCCAGGGATCTGCGACACTTCAGCCTGCCAAGGAGCTGTGTTAGGGGGAGGTGGGGAAAAGT
CGGGTCCTCGGAGCTCATAAAACAAGGACTTCAGTAGGAAATGGCTCCATATTTCCTACTGAAATACGAG
AGCACTCCAGCAGGCAGCAGGGATGAGCGGTGTGGCTATGGACAGAAATGGACAGTGGCTGGCTACAGTT
GGTCCTTCATGGCCTGCTGCTTGGCCCCAAGAACATCTATTGGCAGCACTGTCACTGTCCCATCTCCAAG
ATTTATGGCAGCCCAATACCTCAGACACAGGCCTTAGGGATGGAGACTTTAAACCCAGCATCTGGGAGGG
ATGGTGTTATCTAAGAGTTTTGTACAATTCCATGAGATAAAAACACTCAAGAGACTTGTTTACATAACAT
TTATATAAAGCCTATGATAGACTACCAATAAAGTGATCTTCTAACCCTTCAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5003028 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5003028 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_002050 same experiment
 EMAGE:29490 same embryo
 EMAGE:31374 same embryo
 EMAGE:31364 same embryo
 EMAGE:31397 same embryo
 EMAGE:29503 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS