EMAGE mouse gene expression spatial database

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EMAGE:31460

Gp38 ( MGI:103098)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_002001_01	euxassay_002001_02	euxassay_002001_03	euxassay_002001_04	euxassay_002001_05

euxassay_002001_06	euxassay_002001_07	euxassay_002001_08	euxassay_002001_09	euxassay_002001_10

euxassay_002001_11	euxassay_002001_12	euxassay_002001_13	euxassay_002001_14	euxassay_002001_15

euxassay_002001_16	euxassay_002001_17	euxassay_002001_18	euxassay_002001_19	euxassay_002001_20

euxassay_002001_21	euxassay_002001_22	euxassay_002001_23	euxassay_002001_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 15 16

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14

pons ventricular layer

strong

homogeneous

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

axial skeleton

strong

regional

strong expression: see section 08 09 10 11 12

axial skeleton tail region

strong

regional

strong expression: see section 13 14 15 16

rest of cerebellum ventricular layer

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 14 15 16 17 18 19 20

spinal cord ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 12

submandibular gland primordium

strong

regional

strong expression: see section 06 07 08 15 16 17

medulla oblongata alar plate ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14

telencephalon ventricular layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 16

hypothalamus ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 11 12 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4583
Entity Detected:	Gp38, ( MGI:103098)
Sequence:	sense strand is shown >T4583 GCTTGCTGCCTCTGCGAGTCCAGAAAGCCCGGGCACTCTCTGGCGCTGAGACTTTTGCTCAGCGCCTTCC AACCTCCTCCCGAGCTCTTCCCGGCTGGGCCTGTGGCTTCGAAGTTTTTTGTTTTGTTTTGTTTTTCATC TTTTCACAACCCACAAAAAACCCACTAGCTGCTGAGGCTCCAACGAGACCAAGATGTGGACCGTGCCAGT GTTGTTCTGGGTTTTGGGGAGCGTTTGGTTCTGGGACTCTGCGCAGGGAGGGACTATAGGCGTGAATGAA GATGATATTGTGACCCCAGGTACAGGAGACGGCATGGTGCCCCCAGGTATAGAAGACAAAATAACAACCA CAGGTGCTACTGGAGGGCTTAATGAATCTACTGGCAAGGCACCTCTGGTACCAACGCAGAGAGAGCGTGG GACGAAGCCTCCCTTAGAGGAACTGTCCACCTCAGCAACCTCAGACCATGATCACAGAGAACACGAGAGT ACAACCACTGTCAAAGTGGTGACTAGCCACTCTGTGGACAAGAAAACAAGTCACCCCAATAGAGATAATG CAGGGGATGAAACGCAGACAACAGATAAGAAAGATGGCTTGCCAGTAGTCACCCTGGTTGGAATCATAGT TGGCGTCTTGTTAGCCATTGGCTTCGTCGGAGGGATCTTCATTGTTGTTATGAAGAAGATTTCTGGAAGG TTCTCGCCCTAAAGAGCTAAACAGAACAGGTTGTTCTCCCAACACATCTGAAAATAAGAGAGCTTCCAAC TTGCCCTGTTCCCCATCCTCTTCCTGTGGAGGAAGATGACCCATGGCGTGCCCACCCACCCCACCCACAG CCATGGTGACCCCTCCGCTTGGCCAGCAGTACCAAAGGAAAGATACAGGACAAGCCACAGCCCCTCAAAG CATCTGCCTTTGGAAAACTAAATTTTTAACATAAATGTTATGATCGATGATTCAAAAGACAACATGCTTA GAAAATGGAGCAAAGCCGAGACAGTATCGCCAGCTTTAGCAACTGTATCCATGGGTTCACCCTGGCCAGT CCCTATTACTGTTAAGAGGCATAGAGTCTGGAAATGTTACATCACAGAGGCATGGATCCTTTTGCAGTTG CCAACTTGTAACTGCACTTGATACATACACATTCCAGCCCAGTCCGAAGCATCCACTGTGCCTTCAGTTC CGGGGAGACACTGCTTTTTTTTTTTTTTTACAAGTCTCCAAACCATCTCTAAGCCTCCAATGAGTTGCGA AGCCACGTCCTCATCTCCCTAGGATAGGACTCAAGCCCTCAGATCAGCTGCATCTTTCTGGATAATAGAG ACCTCATCCTAGATGCTGAGAACACTGTTGGATGTCACAAGATGCTAAAAGGCTCAGCCGCTGTAGAACC AAGAACATTTCAGTAGAAGTGACTCGTGTTGGCTTTAGTATAGGAAGTCAGACCGACCAGTTTCTAACAC CCGCCTTCTGCTTCTCAGGACAGACAGGGAAAGCAAAAATAATCTTGTATAATCTTAACTGCTTTTATGG GGTGGATGTAAAGGTTTGCGGTTAATAGAAGATATTTCCAGTCTTCGTGACACGGGGACTCAGTGTTGCT GGGACAGAGCTGTAGTGTCTTGGTCGTCTGTGTTGGTACGCAATACAGTGTAGACATCTTTTCAAATAAA ACCTGATTGTGTCCAAGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4985288 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4985288 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_002001 same experiment
	EMAGE:31518 same embryo
	EMAGE:32192 same embryo
	EMAGE:31827 same embryo
	EMAGE:31721 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source