EMAGE mouse gene expression spatial database

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EMAGE:31540

BC055811 ( MGI:2681842)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006157_01	euxassay_006157_02	euxassay_006157_03	euxassay_006157_04	euxassay_006157_05

euxassay_006157_06	euxassay_006157_07	euxassay_006157_08	euxassay_006157_09	euxassay_006157_10

euxassay_006157_11	euxassay_006157_12	euxassay_006157_13	euxassay_006157_14	euxassay_006157_15

euxassay_006157_16	euxassay_006157_17	euxassay_006157_18	euxassay_006157_19	euxassay_006157_20

euxassay_006157_21	euxassay_006157_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord mantle layer

strong

regional

strong expression: see section 06 07 08 09 10 11 12 moderate expression: see section 13 14 15

olfactory cortex marginal layer

strong

regional

strong expression: see section 11 12 13 16 17 moderate expression: see section 10

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 14 weak expression: see section 08

rest of cerebellum mantle layer

strong

regional

strong expression: see section 08

rest of cerebellum marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 09 10 11 12 13 14 15 16 17 18 19

pons marginal layer

moderate

regional

moderate expression: see section 16

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05 06 16

thoracic ganglion

weak

regional

weak expression: see section 09 12 13

pons mantle layer

strong

regional

strong expression: see section 04 moderate expression: see section 05 06 07 08 09 10 11 12 14 15 17 18

retina

moderate

regional

moderate expression: see section 01 22 weak expression: see section 02 03 04

thalamus mantle layer

strong

regional

strong expression: see section 09 10 11 12 14 15 16 moderate expression: see section 13

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16

dorsal root ganglion

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 weak expression: see section 05 06 07

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 12 moderate expression: see section 05 06 08 09 10 11 13 14 15

cerebral cortex marginal layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 17 18 19 20 21 22 moderate expression: see section 01 10 11 12 13 14 15 16

cerebral cortex mantle layer

strong

regional

strong expression: see section 06

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 17 18

cervical ganglion

moderate

regional

moderate expression: see section 15 weak expression: see section 07

corpus striatum

moderate

regional

moderate expression: see section 03 04 05 06 08 09 10 17 18 19 20 21 weak expression: see section 07

telencephalon mantle layer

strong

regional

strong expression: see section 13 14 moderate expression: see section 11 12 15 16 weak expression: see section 06

midbrain mantle layer

strong

graded

strong expression: see section 10 11 12 moderate expression: see section 05 06 07 08 09 13 14 15 16

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 07 08 09 10 11 13 14 15 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8865
Entity Detected:	BC055811, ( MGI:2681842)
Sequence:	sense strand is shown >T8865 GGCAGAGCTTCGGCTCCAAACTCCGCCACTGCCGCCACGGATCCGACTCCGGCCGCCGTGGCCGCCTATC GCAGCCAACCAGCCGGGAGCAGCTGCGCGCCCCAGCCAGGTCCGGTGCAGCCCGGAGCAGCCAGTGCGTC TGGTGCAGCCCGGAGCATCCTATTACAGCCCAGAGTAGCCTGGTGGTGCAGCCCGGAGCATCCCGGTACA GCCCAGAGCAGCCCCGGGTAGCCTGGCGGCCGGGCTACAGTAGCATGGGGACCTCCCCTCGGCTCTCCTC GCCTCTCGTCACCGCCACGGCCGGCGGCCCGGGTCACAAGCGCTTCTGAGCCCATGGCGAGGGGACCCAC CGCCGCCACTGCGATCGCTGCCGCCGCCGCCGCCTCGGCCAGCGCCGCCAGCTTCTGGACACCATGCAAG CTGCTCCAAGCCTCCGCCGCGCTTCCTGCCTGCTGCTCGCCGCGATTCTGGACCTGGCACGCGGCTACCT GACAGTCAACATTGAGCCCCTTCCCCCTGTGGTGGCTGGGGACGCAGTGACCCTGAAGTGTAACTTCAAG ACAGACGGCCGTATGCGTGAGATCGTGTGGTACCGGGTGACAGATGGCGGCACCATCAAGCAGAAGATCT TCACGTTTGACGCCATGTTCTCCACGAACTACTCGCACATGGAGAATTACCGCAAGAGGGAGGACCTTGT GTATCAGTCCACCGTGAGGCTGCCCGAGGTCCGGATCTCAGACAATGGTCCCTATGAGTGCCACGTGGGT ATCTACGACCGAGCCACGAGGGAGAAGGTGGTCCTCGCCTCAGGCAACATCTTCCTCAATGTCATGGCTC CTCCCACCTCCATCGAAGTCGTGGCTGCTGACTCCCCTGCCCCCTTTAGCCGGTACCAGGCCCAGAACTT CACACTGGTCTGCATCGTGTCAGGAGGGAAGCCGGCGCCCATGGTTTATTTCAAACGGGACGGGGAGCCG ATAGATGCCGTGCCCCTGACAGAACTGCCAGCAGCCAGCTCTGGCCCAGTCCAGGACAGCAGGCCCTTCC GAAGCCTTCTGCACCGAGACGTGGATGATACCAAAATGCAAAAGTCACTGTCCCTTCTGGACACTGAATA CCGGGCTGGACGTCCCTACACGGAGCGCCCCGCGCGCAGCCTGACCCAGGACCCCAGCCTCTTCGTGCAG CCCACCACGGAAAACATCCCAGAGACAGTGGTGAGCCGAGAGTTCCCCCGTTGGGTACACAGCGCCGAGC CGGTCTACTTCCTGCGCCACAGCCGCACCCCAGGCAGCGATGGCACGGTGGAGGTGCGGGCCCTGCTCAC CTGGACCCTCAACCCGCAGATCGACAATGAGGCCCTCTTCAGCTGTGAGGTCAAGCACCCAGCGCTGTCC ATGCCCATGCAGGCGGAGGTCACGCTGGTTGCTCCCAAAGGACCCAAAATCATGATGACGCCCAGCAGAG CCCGGGTTGGGGACACAGTGAGGATCCTAGTCCACGGATTTCAGAATGAGGTCTTCCCAGAACCCATGTT CACGTGGACGAGAGTGGGCAGCCGCCTCCTGGATGGCAGTGCCGAGTTTGACGGGAAGGAGCTTGTGCTG GAGCGGGTTCCTGCCGAGCTCAACGGCTCCATGTACCGCTGCACTGCCCAGAACCCCCTGGGCTCCACTG ATACACACACTCGGCTCATCGTGTTCGAAAACCCAAATATCCCAAGAGGAACAGAGGACTCCCGTGGTTC TGCTTCCGGCCCCGCTGGCGTCCGGCTCACCCTGGTGCTTGCCCTGACAGTGATCCTGGAGCTCACGTGA AGATGCTGCCTCCTCCCGATGCTCCAGCCGCCCGGCATCTCTGCGATCGATTTTCATTTCTTTTCTAAAC TATTTCCAGTCTTGTTCTTAGTCTCGGCCCATGTCTTGGCTTCCTCACTGGGTTTAATTAAACAAACAGA CCAATTTTCCCCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6414125 using vector (pYX-Asc) specific primers. Forward Primer - name:RZ. T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6414125 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006157 same experiment
	EMAGE:29747 same embryo
	EMAGE:29581 same embryo
	EMAGE:29752 same embryo
	EMAGE:29578 same embryo
	EMAGE:29746 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source