EMAGE mouse gene expression spatial database

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EMAGE:31567

4930430F08Rik ( MGI:1921197)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000838_01	euxassay_000838_02	euxassay_000838_03	euxassay_000838_04	euxassay_000838_05

euxassay_000838_06	euxassay_000838_07	euxassay_000838_08	euxassay_000838_09	euxassay_000838_10

euxassay_000838_11	euxassay_000838_12	euxassay_000838_13	euxassay_000838_14	euxassay_000838_15

euxassay_000838_16	euxassay_000838_17	euxassay_000838_18	euxassay_000838_19	euxassay_000838_20

euxassay_000838_21

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

weak

regional

weak expression: see section 08 09 15 16 17

facial vii ganglion

weak

homogeneous

weak expression: see section 03 17

medulla oblongata basal plate

weak

homogeneous

weak expression: see section 06 08 13 14 15

thymus primordium

weak

homogeneous

weak expression: see section 11 12 13 14

trigeminal v ganglion

weak

homogeneous

weak expression: see section 03 04 05 06 15 16 17 18 19 20

telencephalon ventricular layer

weak

homogeneous

weak expression: see section 01 02 03 04 05 06 08 09 11 12 14 15 17 18 19 20

liver lobe

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 08 09 11 12 13 14 15 16 17 18 19 20 21

vestibulocochlear viii ganglion vestibular component

weak

homogeneous

weak expression: see section 04 05 06 17 18

superior glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 16

vestibulocochlear viii ganglion cochlear component

weak

homogeneous

weak expression: see section 05 17

inferior glossopharyngeal ix ganglion

weak

homogeneous

weak expression: see section 06 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T176
Entity Detected:	4930430F08Rik, ( MGI:1921197)
Sequence:	sense strand is shown >T176 GCAGTTAGGATCTTCTGGCTGGTGTTTGGATTCTAGCTCATTTCTGCAGACTTCCTCCGCCCGGGGCCCA GCGCAGTGGAGACAGGTTGCGCGTGGGCAACATGAAGCGCCTGGGCTCGGTGCAGCGGAAGATGCCGTGT GTGTTTGTGACGGAGGTGAAAGCTGAGCCTTCAGCCAAGCGGGAGCATCAGCCATTTAAAGTTTTGGCAA CTGAAACTCTAAGTGAAAAGGCGTTAGATGCAGACGTGTACAATGCGGTTGCAACAGAAAAAGTGGATGG AACATGCTGCTATGTTACTAACTACAAAGGTCAGCCATACCTTTGGGCTCGACTAGATAGAAAACCTAAC AAGCAGGCTGACAAGAGATTTAAAAAATTTCTACATTCAAAAGAAAGTGCAAAAGAGTTTCACTGGAACA CTGAGGAAGACTTCAAGCCTGTCCCAGAGTGCTGGATACCAGCA
Notes:	The probe template was PCR amplified from IMAGE:2646347 using vector specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2646347 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000838 same experiment
	EMAGE:31823 same embryo
	EMAGE:30110 same embryo
	EMAGE:31512 same embryo
	EMAGE:30742 same embryo
	EMAGE:31489 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source