EMAGE mouse gene expression spatial database

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EMAGE:31606

Tlr2 ( MGI:1346060)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005029_01	euxassay_005029_02	euxassay_005029_03	euxassay_005029_04	euxassay_005029_05

euxassay_005029_06	euxassay_005029_07	euxassay_005029_08	euxassay_005029_09	euxassay_005029_10

euxassay_005029_11	euxassay_005029_12	euxassay_005029_13	euxassay_005029_14	euxassay_005029_15

euxassay_005029_16	euxassay_005029_17	euxassay_005029_18	euxassay_005029_19	euxassay_005029_20

euxassay_005029_21	euxassay_005029_22

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

knee mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 13 14 15 16 17 18

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6874
Entity Detected:	Tlr2, ( MGI:1346060)
Sequence:	sense strand is shown >T6874 CGGAGCCTCTGGACTTTCAGTTCTGTTTTGCCTGCCCTGTGGCTCCTGCCAGCTCTGATGCCAGGCTCCG TTCCCCTTGCAGACCCCTGTGGGCGGCGCTTGCCGCACCGGGGGCGGTGCTGGCGACCGGGAAGTTCGGG GCCCTGACCTGGGGACATCCCCTTCCCTCACTTCCAGGTCTTCAGTCTTCCTAGGCTGGTGCCCAGATGG CTAGTGGGCACGGGGAGCGGCGGCTGGAGGACTCCTAGGCTCCGGGCAGGCGGTCACTGGCAGGAGATGT GTCCGCAATCATAGTTTCTGATGGTGAAGGTTGGACGGCAGTCTCTGCGACCTAGAAGTGGAAAAGATGT CGTTCAAGGAGGTGCGGACTGTTTCCTTCTGACCAGGATCTTGTTTCTGAGTGTAGGGGCTTCACTTCTC TGCTTTTCGTTCATCTCTGGAGCATCCGAATTGCATCACCGGTCAGAAAACAACTTACCGAAACCTCAGA CAAAGCGTCAAATCTCAGAGGATGCTACGAGCTCTTTGGCTCTTCTGGATCTTGGTGGCCATAACAGTCC TCTTCAGCAAACGCTGTTCTGCTCAGGAGTCTCTGTCATGTGATGCTTCTGGGGTGTGTGATGGCCGCTC CAGGTCTTTCACCTCTATTCCCTCCGGACTCACAGCAGCCATGAAAAGCCTTGACCTGTCTTTCAACAAG ATCACCTACATTGGCCATGGTGACCTCCGAGCGTGTGCGAACCTCCAGGTTCTGATTTTGAAGTCCAGCA GAATCAATACAATAGAGGGAGACGCCTTTTATTCTCTGGGCAGTCTTGAACATTTGGATTTGTCTGATAA TCACCTATCTAGTTTATCTTCCTCCTGGTTCGGGCCCCTTTCCTCTTTGAAATACTTAAACTTAATGGGA AATCCTTACCAGACACTGGGGGTAACATCGCTTTTTCCCAATCTCACAAATTTACAAACCCTCAGGATAG GAAATGTAGAGACTTTCAGTGAGATAAGGAGAATAGATTTTGCTGGGCTGACTTCTCTCAATGAACTTGA AATTAAGGCATTAAGTCTCCGGAATTATCAGTCCCAAAGTCTAAAGTCGATCCGCGACATCCATCACCTG ACTCTTCACTTAAGCGAGTCTGCTTTCCTGCTGGAGATTTTTGCAGATATTCTGAGTTCTGTGAGATATT TAGAACTAAGAGATACTAACTTGGCCAGGTTCCAGTTTTCACCACTGCCCGTAGATGAAGTCAGCTCACC GATGAAGAAGCTGGCATTCCGAGGCTCGGTTCTCACTGATGAAAGCTTTAACGAGCTCCTGAAGCTGTTG CGTTACATCTTGGAACTGTCGGAGGTAGAGTTCGACGACTGTACCCTCAATGGGCTCGGCGATTTCAACC CCTCGGAGTCAGACGTAGTGAGCGAGCTGGGTAAAGTAGAAACAGTCACTATCCGGAGGTTGCATATCCC CCAGTTCTATTTGTTTTATGACCTGAGTACTGTCTATTCCCTCCTGGAGAAGGTGAAGCGAATCACAGTA GAGAACAGCAAGGTCTTCCTGGTTCCCTGCTCGTTCTCCCAGCATTTAAAATCATTAGAATTCTTAGACC TCAGCGAAAATCTGATGGTTGAAGAATATTTGAAGAACTCAGCCTGTAAGGGAGCCTGGCCTCCTCTACA AACCTTAGTTTTGAGCCAGAATCATTTGAGATCAATGCAAAAAACAGGAGAGATTTTGCTGACTCTGAAA AACCTGACCTCCCTTGACATCAGCAGGAACACTTTTCATCCGATGCCCGACAGCTGTCAGTGGCCAGAAA AGATGCGCTTCCTGAATTTGTCCAGTACAGGGATCCGGGTGGTAAAAACGTGCATTCCTCAGACGCTGGA GGTGTTGGATGTTAGTAACAACAATCTTGACTCATTTTCTTTGTTCTTGCCTCGGCTGCAAGAGCTCTAT ATTTCCAGAAATAAGCTGAAAACACTCCCAGATGCTTCGTTGTTCCCTGTGTTGCTGGTCATGAAAATCA GAGAGAATGCAGTAAGTACTTTCTCTAAAGACCAACTTGGTTCTTTTCCCAAACTGGAGACTCTGGAAGC AGGCGACAACCACTTTGTTTGCTCCTGCGAACTCCTATCCTTTACTATGGAGACGCCAGCTCTGGCTCAA ATCCTGGTTGACTGGCCAGACAGCTACCTGTGTGACTCTCCGCCTCGCCTGCACGGCCACAGGCTTCAGG ATGCCCGGCCCTCCGTCTTGGAATGTCACCAGGCTGCACTGGTGTCTGGAGTCTGCTGTGCCCTTCTCCT GTTGATCTTGCTCGTAGGTGCCCTGTGCCACCATTTCCACGGGCTGTGGTACCTGAGAATGATGTGGGCG TGGCTCCAGGCCAAGAGGAAGCCCAAGAAAGCTCCCTGCAGGGACGTTTGCTATGATGCCTTTGTTTCCT ACAGTGAGCAGGATTCCCATTGGGTGGAGAACCTCATGGTCCAGCAGCTGGAGAACTCTGACCCGCCCTT TAAGCTGTGTCTCCACAAGCGGGACTTCGTTCCGGGCAAATGGATCATTGACAACATCATCGATTCCATC GAAAAGAGCCACAAAACTGTGTTCGTGCTTTCTGAGAACTTCGTACGGAGCGAGTGGTGCAAGTACGAAC TGGACTTCTCCCACTTCAGGCTCTTTGACGAGAACAACGACGCGGCCATCCTTGTTTTGCTGGAGCCCAT TGAGAGGAAAGCCATTCCCCAGCGCTTCTGCAAACTGCGCAAGATAATGAACACCAAGACCTACCTGGAG TGGCCCTTGGATGAAGGCCAGCAGGAAGTGTTTTGGGTAAATCTGAGAACTGCAATAAAGTCCTAGGTTC TCCACCCAGTTCCTGACTTCCTTAACTAAGGTCTTTGTGACACAAACTGTAACAAAGTTTATAAGTAACA TAGAATTGTATTATTGAGGATATTAACTATGGGTTTTGTCTTGAATACTGTTATATAAATATGTGACATC AGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3966249 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3966249 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005029 same experiment
	EMAGE:30901 same embryo
	EMAGE:31615 same embryo
	EMAGE:31184 same embryo
	EMAGE:31026 same embryo
	EMAGE:30216 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source