EMAGE mouse gene expression spatial database

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EMAGE:31650

Thbs2 ( MGI:98738)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005000_01	euxassay_005000_02	euxassay_005000_03	euxassay_005000_04	euxassay_005000_05

euxassay_005000_06	euxassay_005000_07	euxassay_005000_08	euxassay_005000_09	euxassay_005000_10

euxassay_005000_11	euxassay_005000_12	euxassay_005000_13	euxassay_005000_14	euxassay_005000_15

euxassay_005000_16	euxassay_005000_17	euxassay_005000_18	euxassay_005000_19	euxassay_005000_20

euxassay_005000_21	euxassay_005000_22	euxassay_005000_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 weak expression: see section 05 13 14 15 16

lateral ventricle choroid plexus

moderate

regional

moderate expression: see section 09 10 12 weak expression: see section 06 07 08 13 14 15 16

medulla oblongata floor plate

strong

regional

strong expression: see section 09

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

metencephalon floor plate

strong

regional

strong expression: see section 10

midbrain roof plate

strong

regional

strong expression: see section 11

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8043
Entity Detected:	Thbs2, ( MGI:98738)
Sequence:	sense strand is shown >T8043 AAGAGGTAATCCCCTCTCCTCTCCTGCCGCAGCTAGTCCCCCTCAGGACAGACAGAGTACTGGCGTCGGT CACCATTCACTTGCAAACACACCAGGTCACGTGAAGAAACTTCCTGGTGACACTCAGGCTGTAGCTGTGC ACTCTTCAACCACGAGGTTGGTTTTCTCCTAAGTGTCACAGGTGGAGACAAGATGCTCTGGGCACTGGCC CTGCTGGCTCTGGGCATAGGGCCAAGAGCTTCTGCTGGTGACCACGTCAAGGACACTTCATTTGACCTTT TCAGCATCAGCAACATTAACCGGAAGACCATCGGTGCCAAGCAGTTCCGAGGGCCTGACCCCGGGGTGCC CGCCTACCGTTTTGTACGGTTTGACTACATCCCCCCAGTGAACACAGATGATCTCAACAGGATTGTCAAG CTTGCAAGGAGAAAGGAGGGCTTCTTCCTCACAGCCCAACTGAAGCAGGACCGCAAGTCTCGGGGAACGC TCCTGGTGTTGGAAGGCCCCGGCACCTCCCAGAGGCAGTTTGAGATTGTGTCCAATGGCCCAGGGGACAC TTTGGACCTCAACTACCGGGTAGAAGGCAATCAGCATACCAACTTCCTGGAGGATGTGGGCCTGGCTGAC TCCCAGTGGAAGAATGTGACTGTGCAGGTGGCCAGTGACACCTATAGCCTGTATGTGGGCTGCGATCTTA TCGACAGTGTCACCCTGGAAGAACCATTCTATGAGCAGCTAGAAGTAGACAGGAGCAGGATGTACGTGGC CAAAGGTGCATCTCGAGAGAGTCACTTCAGGGGCTTGCTGCAGAATGTCCATCTCGTGTTTGCAGATTCT GTGGAAGATATCTTAAGCAAGAAAGGCTGTCAACACAGCCAGGGAGCTGAAGTCAACACCATCAGTGAAC ATACAGAGACTCTCCATCTGAGCCCTCACATCACCACAGATCTCGTGGTCCAGGGTGTGGAGAAGGCACA GGAGGTGTGTACGCACTCCTGCGAGGAGTTGAGCAACATGATGAATGAGCTCTCTGGACTGCACGTCATG GTGAACCAGCTGAGCAAGAACCTGGAGAGAGTGTCTAGTGATAACCAGTTCCTTTTGGAGCTCATTGGGG GCCCTCTGAAGACAAGAAACATGTCAGCCTGTGTGCAGGAGGGCCGAATCTTTGCAGAAAATGAAACCTG GGTTGTGGATAGTTGTACCACATGCACCTGCAAGAAATTTAAAACAGTCTGCCATCAGATCACCTGCTCA CCTGCAACTTGTGCCAACCCATCTTTTGTGGAAGGCGAGTGCTGTCCATCCTGTTCACACTCTGCAGACA GTGATGAGGGCTGGTCTCCGTGGGCAGAGTGGACCGAGTGTTCTGTCACCTGTGGCTCTGGGACCCAGCA GAGAGGCCGGTCTTGTGATGTCACCAGCAACACCTGCCTGGGCCCCTCCATTCAGACAAGGACATGCAGC CTGGGCAAATGTGATACGAGAATCCGTCAGAATGGAGGCTGGAGTCACTGGTCACCCTGGTCTTCATGCT CCGTGACTTGTGGAGTTGGCAATGTCACCCGCATACGTCTCTGCAACTCACCAGTGCCCCAGATGGGTGG CAAGAACTGCAAGGGCAGCGGCCGGGAAACCAAACCCTGTCAGCGTGATCCGTGCCCAATTGATGGCCGC TGGAGCCCCTGGTCCCCTTGGTCAGCCTGCACAGTTACCTGTGCTGGAGGGATCCGTGAGCGCTCACGTG TTTGCAACAGCCCTGAGCCCCAGTATGGAGGGAAGGACTGTGTCGGGGATGTGACAGAACACCAAATGTG CAACAAGAGAAGCTGCCCTATTGATGGGTGCTTATCCAACCCGTGTTTTCCTGGAGCCAAGTGCAACAGC TTCCCTGATGGGTCCTGGTCCTGTGGCTCCTGCCCAGTGGGCTTTCTGGGCAATGGTACCCACTGTGAGG ACCTGGATGAGTGTGCTGTGGTCACAGATATTTGCTTCTCAACTAACAAAGCTCCCCGCTGTGTCAACAC CAACCCGGGCTTCCACTGCCTGCCTTGTCCACCACGCTACAAGGGGAACCAACCCTTCGGTGTTGGCCTG GAGGATGCTAGGACAGAAAAACAAGTGTGTGAGCCAGAGAATCCATGTAAGGACAAGACTCACAGCTGCC ACAAGAATGCAGAGTGCATCTACCTGGGCCACTTTAGTGACCCCATGTACAAGTGTGAGTGCCAGATTGG CTACGCAGGTGATGGGCTCATCTGCGGGGAGGACTCAGACCTGGATGGCTGGCCCAACAACAACCTGGTG TGTGCTACTAATGCCACCTACCACCGCATCAAGGACAACTGCCCCAAACTGCCAAATTCCGGGCAGGAGG ATTTTGATAAGGATGGAATCGGAGATGCTTGTGACGAGGACGATGACAATGACGGTGTGAGCGATGAGAA GGACAATTGCCAGCTTCTCTTCAATCCCCGTCAATTAGACTATGACAAGGATGAGGTTGGAGACCGCTGT GACAACTGCCCCTATGTGCACAACCCAGCACAGATCGACACAGACAACAATGGCGAGGGGGATGCCTGCT CTGTGGACATTGACGGAGACGATGTTTTCAATGAGCGAGACAATTGTCCATATGTCTACAACACTGACCA GAGAGATACGGATGGTGATGGCGTGGGTGACCGCTGTGACAATTGTCCTCTGATGCACAACCCAGATCAG ATCGATCAGGACAATGATCTCGTTGGAGACCAGTGTGACAACAATGAGGACATAGATGATGACGGCCACC AGAACAACCAAGACAACTGCCCATACATCTCCAACTCCAACCAGGCTGACCATGACAACGACGGCAAGGG CGATGCCTGCGACTCTGATGATGACAATGATGGTGTTCCAGATGACAGGGACAACTGTCGGCTTGTGTTC AACCCAGACCAGGAAGACTCGGACGGTGACGGCCGAGGTGACATTTGTAAAGATGACTTTGACAATGATA ATGTCCCAGATATTGATGATGTGTGCCCTGAGAACAATGCCATCACTGAGACAGACTTCAGAAACTTCCA GATGGTCCCTCTGGATCCCAAGGGGACCACACAAATTGATCCCAACTGGGTAATTCGTCACCAAGGCAAA GAGCTGGTGCAGACAGCAAACTCAGACCCTGGCATCGCTGTAGGTTTCGACGAGTTTGGGTCTGTGGACT TCAGTGGCACTTTCTATGTCAACACTGACCGGGATGATGACTACGCTGGCTTTGTCTTTGGCTATCAGTC AAGCAGCCGCTTCTATGTGGTGATGTGGAAGCAGGTGACCCAGACCTACTGGGAAGACAAGCCCAGTCGG GCTTACGGCTACTCTGGTGTGTCACTCAAAGTGGTAAACTCCACGACTGGTACTGGCGAGCACCTGAGGA ATGCCCTGTGGCGCACGGGAAACACAGAAGGCCAGGTCCGGACTCTATGGCATGACCCCAAAAACATTGG CTGGAAAGACTACACTGCCTACAGGTGGCACCTGATTCACAGGCCTAAGACAGGCTACATGAGAGTCTTA GTGCATGAAGGAAAGCAAGTCATGGCTGACTCAGGACCAATTTATGACCAAACCTACGCTGGTGGACGGC TGGGCCTGTTTGTCTTCTCCCAAGAGATGGTCTATTTCTCGGACCTCAAGTATGAGTGCAGAGATGCCTA GAGAGCAGGGCTCCAGCTCCAGCAATGTGCTGCAAACACCCCTTCTTAGACACATCAGTCCATCTTGGCA CTTGTGGCTTTTCTGTCATTTGGCATTTCCTGTTTCTTGACCTTAACTGAGTGGATCTACACCTCCTTCA TCAGCACCAAGTCCAAGTGTCTTCAAAGGAGAAACATCAATTGCACTCCAAGAGCTTCCAGCCTGCTGCT GGAAAACATTTGGATGAGATATGAGGCTCACCGTGGAGCGAAGACCGAGCATTCCGCTGTGTTGCCTTTT CTTGTTTGTTTAAAAAGAATGACGTTTACATGTAAATGTAATTACTTGCAGTATTTATGTGTATATGGAG TCGAAGGGAGTATTGTGTTTAAGTCATTCTCATAAATTAAGCATGAAAAATAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30062369 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30062369 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005000 same experiment
	EMAGE:30638 same embryo
	EMAGE:32011 same embryo
	EMAGE:31949 same embryo
	EMAGE:31058 same embryo
	EMAGE:31973 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source