EMAGE mouse gene expression spatial database

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EMAGE:31721

Abhd5 ( MGI:1914719)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001995_01	euxassay_001995_02	euxassay_001995_03	euxassay_001995_04	euxassay_001995_05

euxassay_001995_06	euxassay_001995_07	euxassay_001995_08	euxassay_001995_09	euxassay_001995_10

euxassay_001995_11	euxassay_001995_12	euxassay_001995_13	euxassay_001995_14	euxassay_001995_15

euxassay_001995_16	euxassay_001995_17	euxassay_001995_18	euxassay_001995_19	euxassay_001995_20

euxassay_001995_21	euxassay_001995_22	euxassay_001995_23	euxassay_001995_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 05 06 07 08 11 12 13 14 15 16

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 16 17 18 19 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 06 moderate expression: see section 16 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4584
Entity Detected:	Abhd5, ( MGI:1914719)
Sequence:	sense strand is shown >T4584 CCACGCGTCCGGCCGGCTTCGAGGTGTGTCCCTGCGCTTGCGCGGCGGCGGTGATGAAAGCGATGGCGGC GGAGGAGGAGGTGGACTCGGCAGACGCCGGTGGAGGGTCAGGATGGCTGACAGGGTGGCTTCCTACCTGG TGTCCCACATCTACATCACACCTTAAAGAAGCTGAAGAGAAAATGTTAAAATGTGTCCCCTGCACTTACA AGAAAGAGCCTGTGCGCATATCCAATGGAAACAGAATATGGACGCTGATGTTCTCTCACAACATTTCTAG TAAGACGCCACTTGTCCTCCTTCATGGTTTTGGAGGAGGTCTTGGACTTTGGGCCCTGAATTTTGAAGAT CTAAGCACCGATAGGCCTGTCTATGCCTTTGACCTATTGGGCTTCGGAAGAAGTAGTAGACCTAGGTTTG ACAGTGATGCGGAAGAAGTGGAGAATCAGTTTGTGGAATCCATTGAAGAGTGGAGATGTGCCCTCAGGTT GGACAAAATGATCTTGCTTGGACACAACCTGGGAGGGTTCTTGGCTGCCGCTTACTCACTGAAGTACCCA TCAAGGGTTAGTCACCTCATTTTAGTAGAGCCATGGGGTTTTCCTGAGCGACCAGATCTTGCTGATCAAG AGAGACCAATTCCAGTTTGGATCAGGGCCCTAGGGGCAGCATTGACTCCCTTTAACCCCTTGGCTGGCCT CAGGATTGCAGGACCTTTTGGGTTAAGTCTAGTGCAGCGTTTGAGGCCTGATTTCAAGCGGAAGTACTCC TCTATGTTTGAAGATGACACGGTGACAGAGTACATCTACCACTGTAATGTACAAACCCCAAGTGGTGAGA CAGCTTTCAAAAACATGACGATTCCTTATGGGTGGGCCAAACGGCCAATGCTTCAGCGGATAGGTGGCTT GCATCCTGACATTCCAGTTTCAGTGATCTTTGGAGCCCGATCCTGCATAGATGGCAACTCTGGAACCAGC ATCCAGTCACTGCGACCGAAGTCCTACGTGAAGACAATTGCCATCCTCGGGGCGGGGCATTATGTGTATG CAGATCAGCCAGAAGAATTCAACCAGAAAGTCAAGGAGATCTGCCACACAGTAGACTGAGCACACAGAAC CATGCAGCATCCGTGACGGGTGCCGTTCATGAGCAATCCCCACAGCCCGAGGACCCCCGCAGTCTCCTGG ACTGGACTCTGCAGCTTTCCACATTTAAACCAACCAGTGCCTTCTAGAAGAATGGCTTTCCTTTCTCCTA CACAGAATTAAAATATAGGAGAGTCTCCCAATTTAATAATCGCCTTAAATAAAGGTTGTCCTTCTGATGT AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4985992 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4985992 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001995 same experiment
	EMAGE:31518 same embryo
	EMAGE:32192 same embryo
	EMAGE:31460 same embryo
	EMAGE:31827 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source