EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31795

Praf2 ( MGI:1859607)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001741_01	euxassay_001741_04	euxassay_001741_05	euxassay_001741_06	euxassay_001741_07

euxassay_001741_08	euxassay_001741_09	euxassay_001741_10	euxassay_001741_11	euxassay_001741_12

euxassay_001741_13	euxassay_001741_14	euxassay_001741_15	euxassay_001741_16	euxassay_001741_17

euxassay_001741_18	euxassay_001741_19	euxassay_001741_20	euxassay_001741_21	euxassay_001741_22

euxassay_001741_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 14 15 weak expression: see section 10

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 09 15 16 17 18 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 17

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2628
Entity Detected:	Praf2, ( MGI:1859607)
Sequence:	sense strand is shown >T2628 TGGCCTCGAGCCAGATTCGGACGAGGATCACTGCAGCCCCAACTAGTCATCTCTTAGCCTCCAGCCCCTG TTACCTAGGGACCAAAGTCTTCCTCGGTCCTGAATTTGAGACTCAGTCACCTACTTCCAGTCTTGAAACT GGACTTTTAAGCCAGAGCATCTGTGGCCAATCTCAAAACTGGACCCCAAGATACTCCTCTGACCCAAATG TGGGACTCACTAGTCTTCCATACGTCAAAGCTAGGCCCTAAGGCAGGACATTCTCAAATCTTGAGCTCTA AAGATTTTTCAGACCCCACACAGCTTGGAATATAGGTTATAAGACCTGGCCAAATCTTGAGCCTAATGAG TCCACACTGAGCCCTTAACCCCAGACGACCCTAGCCCTCAGCAGTCCCAATAGCCTCCCTCCAACCGTTA AGAGGGTCTCTTCCTCCCTCACAGTGTTCTCAGTATCAAGGACCAACATGTGGGGCTGGCTAGGGATAGC CCCAACTGGGAAAGGGACACATATAATAGTGTTGCTACAACAATAAAGCCTT
Notes:	The probe template was PCR amplified from IMAGE:1400773 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1400773 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001741 same experiment
	EMAGE:29989 same embryo
	EMAGE:31273 same embryo
	EMAGE:31877 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source