EMAGE mouse gene expression spatial database

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EMAGE:31802

Pdpk1 ( MGI:1338068)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001988_01	euxassay_001988_02	euxassay_001988_03	euxassay_001988_04	euxassay_001988_05

euxassay_001988_06	euxassay_001988_07	euxassay_001988_08	euxassay_001988_09	euxassay_001988_10

euxassay_001988_11	euxassay_001988_12	euxassay_001988_13	euxassay_001988_14	euxassay_001988_15

euxassay_001988_16	euxassay_001988_17	euxassay_001988_18	euxassay_001988_19	euxassay_001988_20

euxassay_001988_21	euxassay_001988_22	euxassay_001988_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 11 12 13 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 18 19

vagus x ganglion

moderate

regional

moderate expression: see section 07 08 15

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 15 16 17 18 19 20

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 16

spinal cord

moderate

homogeneous

moderate expression: see section 07 08 09 10 11 12 13

brain

moderate

homogeneous

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1142
Entity Detected:	Pdpk1, ( MGI:1338068)
Sequence:	sense strand is shown >T1142 TCCTCGAGNCTGTTGGCCTACTGGTTTGCTGGGGCTCCGCCGCGGGGAGGAGGACGCTGAGGAGGCGCCG AGCAGCGCAACGCTGCGGGGGAGGCGCCCGCGCCGACTTGGGGCTCATAGCCAGGACCACCAGTCCAGCT GTATGACGCTGTGCCCATTCTGTCCAGTGTGGTGCTATGTTCCTGCCCATCCCCATCAATGGTGAGGTCC CAGACTGAGCCCGGTTCGTCCCCTGGCATTCCTAGTGGTGTTAGCAGGCAGGGATCCACCATGGATGGCA CCACAGCTGAAGCCCGACCAAGCACCAACCCCTATGGCAGCACCCTGCCCAGCTGCCACCACAGCCTCGC AAGAAACGCCCTGAAGACTTCAAGTTTGGGAAAATTCTTGGCGAGGGCTCTTTTTCAACAGTTGTTCTGG CCCGAGAACTGGCCACTTCCAGAGAATATGCTATTAAAATTCTGGAGAAACGTCATATTATAAAAGAAAA CAAAGTTCCGTATGTAACTAGAGAGAGAGATGTGATGTCACGCCTGGATCACCCCTTCTTTGTGAAACTT TATTTTACATTTCAGGA
Notes:	The probe template was PCR amplified from IMAGE:2135930 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2135930 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001988 same experiment
	EMAGE:31010 same embryo
	EMAGE:30575 same embryo
	EMAGE:31794 same embryo
	EMAGE:29315 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source