Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:31810

Rps27l ( MGI:1915191)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810
euxassay_013950_01 euxassay_013950_02 euxassay_013950_03 euxassay_013950_04 euxassay_013950_05
EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810
euxassay_013950_06 euxassay_013950_07 euxassay_013950_08 euxassay_013950_09 euxassay_013950_10
EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810
euxassay_013950_11 euxassay_013950_12 euxassay_013950_13 euxassay_013950_14 euxassay_013950_15
EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810 EMAGE:31810
euxassay_013950_16 euxassay_013950_17 euxassay_013950_18 euxassay_013950_19 euxassay_013950_20

View assay images: EMAGE genex expression entry
Expression pattern clarity: one star
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
seminiferous cord
strong strong
regionalstrong expression: see section 04 05 06 16 17 18
metanephros
strong strong
regionalstrong expression: see section 05 06 07 08 13 14 15 16
left lung
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07
rest of cerebellum ventricular layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
pancreas
strong strong
regionalstrong expression: see section 07 08 09
lower jaw molar
strong strong
regionalstrong expression: see section 05 06 07 08 16 17 18
upper jaw molar
strong strong
regionalstrong expression: see section 05 06 07 08 16 17 18
leg muscle
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 18 19 20
vertebral axis musculature
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
thymus primordium
strong strong
regionalstrong expression: see section 07 08 09 10 11
arm muscle
strong strong
regionalstrong expression: see section 01 02 17 18 19 20
lower leg rest of mesenchyme
strong strong
regionalstrong expression: see section 01 02
vibrissa
strong strong
regionalstrong expression: see section 03 04 05 06 19 20
telencephalon ventricular layer
strong strong
regionalstrong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain ventricular layer
strong strong
regionalstrong expression: see section 06 07 08 09 10 11 12 13 14 15 16
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 14 15 16 17 18
right lung
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18
medulla oblongata basal plate ventricular layer
strong strong
regionalstrong expression: see section 10 11 12 13 14 15
tongue
strong strong
regionalstrong expression: see section 09 10 11 12 13 14
pons ventricular layer
strong strong
regionalstrong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
anterior naris epithelium
strong strong
regionalstrong expression: see section 11 12 15 16
spinal cord ventricular layer
strong strong
regionalstrong expression: see section 07 08 09 10 11 12 13
submandibular gland primordium
strong strong
regionalstrong expression: see section 04 05 06 14 15 16 17
vomeronasal organ
strong strong
regionalstrong expression: see section 14 16
medulla oblongata alar plate ventricular layer
strong strong
regionalstrong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17
liver
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
lower jaw incisor
strong strong
regionalstrong expression: see section 09 10 12 13 14 15
upper jaw incisor
strong strong
regionalstrong expression: see section 10 11 14 15
diencephalon lateral wall ventricular layer
strong strong
regionalstrong expression: see section 11 12 13 14 15
Annotation Validation: text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T598
Entity Detected:Rps27l, ( MGI:1915191)
Sequence:sense strand is shown

>T598
CCTCGAGCCTGTTGGCCTACTGGGGAAGTTGCTGAGGGGCGCTCGAAAGCGAGCAGTTCGTCTCGGCGAC
CTGGAGCGGCCGCGCTTGCGGAGCCGTCACCATGCCCCTGGCTAGAGATCTGTTACACCCTTCCTTGGAG
GAGGAGAAGAAAAAACATAAGAAGAAACGGCTGGTTCAGAGCCCAAATTCTCACTTCATGGATGTGAAAT
GTCCAGGTTGCTACAAGATTACTACAGTTTTCAGCCATGCACAGACTGTGGTTCTTTGTGTGGGTTGTTC
AACTGTGTTGTGCCAGCCCACAGGAGGAAAAGCCAGGCTCACAGAAGGCTGTTCATTTAGAAGAAAGCAA
CACTAATGAGCTATGAAGTTCCGGAATTTGTGTTTTTCACAGAAAGCCTTACCAACTTCAGTTACTTTAC
CAAGACAATGTAATTATTGTTTGATTTTATAAAGTCTACAACAATGATCTCCTATTTTGGTGTCAGTTTT
TCAATAAAGTTTTAATTATGGAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1885690 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1885690 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EurExpress:euxassay_013950 same experiment
 EMAGE:31791 same embryo
 EMAGE:30915 same embryo
 EMAGE:30805 same embryo
 EMAGE:31776 same embryo
 EMAGE:30874 same embryo
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS