EMAGE mouse gene expression spatial database

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EMAGE:31821

Calb1 calbindin 1 ( MGI:88248)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_007049_01	euxassay_007049_02	euxassay_007049_03	euxassay_007049_04	euxassay_007049_05

euxassay_007049_06	euxassay_007049_07	euxassay_007049_08	euxassay_007049_09	euxassay_007049_10

euxassay_007049_11	euxassay_007049_12	euxassay_007049_13	euxassay_007049_14	euxassay_007049_15

euxassay_007049_16	euxassay_007049_17	euxassay_007049_18	euxassay_007049_19	euxassay_007049_20

euxassay_007049_21	euxassay_007049_22	euxassay_007049_23	euxassay_007049_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

spinal cord mantle layer

strong

spotted

strong expression: see section 09 10 11 12 13 14 15 16 17 18

inner ear

strong

regional

strong expression: see section 06 07 08 09 18 19 20

right lung

moderate

regional

moderate expression: see section 16 17 18 19 20 21 22 23 24

stomach

strong

single cell

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11

metanephros

strong

regional

strong expression: see section 08

rest of cerebellum mantle layer

strong

spotted

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

glossopharyngeal ix ganglion

moderate

spotted

moderate expression: see section 19

left lung

strong

regional

strong expression: see section 04 05 08 moderate expression: see section 06 07 09 10 11 12 13 14 15

pons mantle layer

strong

spotted

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midgut

strong

single cell

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

perioptic mesenchyme

moderate

regional

moderate expression: see section 04 05 06 07 08 21 22 23 24

dorsal root ganglion

strong

spotted

strong expression: see section 08 09 10 11 12 13 14 15 17 18 19

skin

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

medulla oblongata basal plate mantle layer

strong

spotted

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

vagus x ganglion

moderate

spotted

moderate expression: see section 08 18 19

pharyngo-tympanic tube

strong

regional

strong expression: see section 01 02 03 04 23 24

trigeminal v ganglion

strong

spotted

strong expression: see section 06 21 22 23 moderate expression: see section 04 05 07 08 09 10 18 19 20

renal cortex

strong

regional

strong expression: see section 09 10 11 12 18 19 20 21 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 10 13 20 moderate expression: see section 09 11 12 14 15 16 17 18 19 21

midbrain marginal layer

strong

spotted

strong expression: see section 09

telencephalon mantle layer

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

midbrain mantle layer

strong

spotted

strong expression: see section 08 10 11 12 13 14 15 16 17 18 19

diencephalon lateral wall mantle layer

strong

spotted

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4341
Entity Detected:	Calb1, calbindin 1 ( MGI:88248)
Sequence:	sense strand is shown >T4341 CCCCTTACCAGCCCTCTCGCCCGAGGTTCGCGCTCCGCGCACTCTCAAACTAGCCGCTGCACCACGATGG CAGAATCCCACCTGCAGTCATCTCTGATCACAGCCTCACAGTTTTTTGAGATCTGGCTTCATTTCGACGC TGACGGAAGTGGTTACCTGGAAGGAAAGGAGCTGCAGAACTTGATCCAGGAGCTTCTGCAGGCGCGAAAG AAGGCTGGATTGGAGCTATCACCGGAAATGAAATCCTTTGTGGATCAATATGGACAGAGAGATGATGGAA AAATAGGAATTGTAGAGTTGGCTCACGTCTTACCCACAGAAGAGAATTTCTTGCTGCTCTTTCGATGCCA GCAACTGAAGTCCTGCGAGGAATTCATGAAGACTTGGAGAAAGTATGATACTGACCACAGCGGCTTCATC GAAACCGAGGAACTTAAGAACTTTCTAAAGGACCTACTAGAGAAAGCAAACAAGACTGTGGATGATACAA AACTAGCAGAGTACACAGACCTCATGCTGAAACTATTTGATTCAAATAATGACGGAAAGCTGGAACTGAC AGAGATGGCCAGGTTACTACCAGTGCAGGAAAATTTCCTTCTTAAATTCCAGGGAATCAAAATGTGTGGG AAAGAGTTCAATAAGGCTTTTGAGTTATATGATCAGGATGGCAACGGATACATAGATGAAAATGAGCTGG ATGCTTTGCTGAAAGATCTGTGTGAGAAGAACAAACAGGAATTGGATATTAACAATATTACTACATACAA GAAGAACATAATGGCCTTGTCGGATGGAGGGAAGCTGTACCGAACAGACCTTGCTCTTATTCTTTCTGCT GGAGACAACTAGAGTTGGTGACCACAACCACTTGCTAGTGATACATTGTATCTAAAACCATAACTGTGCG CTATAAAGAAGTAGGCTGTATTTTCTTTTATATCTGTAAATTCTACTGCATATAGAGAACTATTCAGGAT GTGTGGCACATTCTTTTCTGCTTGTTTCTACACTGTTTGTAATGTACAGTTTTTGTAAGCATATAAATGA AAAGAAGAAAGACCATGCTTAGGCCAGTCAGTACAATCAATTTTGAAAAATGAATCTACCATGGTTTTGC GTTTATAAATACAGATGAACACTTGGATTTCCCCGAAAATCTACCATCTCAAAACTTCTAGTGTCAGATT GTAAATGAATATCTGTCATTGAGTAAAAGAATAACTCATGGCAAGCCAACTGTTTTTATTTTAGGCAACC ATAGGACTGTCACCCAAAGCACCTCTGTGCTGCTTCTATCTGGCGGAAGGGATGGGCTTCTGCCTCTGAA GCACCAAATATCAACAGTTAATTATGGCTTTATTCTGAAACGATCTCCCTAGAGATTTAGTGTACTGATC AGTGACATGTCTACTGCTTAAATATAAACCACACTATTGACTCCAGCTGGCTTGGTAAGGGAAGGTAGCC AGATGACACATAACTATGTCTGATTCTATGCCTATATTTCCAAGAAGTCTACTGCCAGAGAGTATGACCA TAGCCCATTTTCTAAATTATTTTCATGTGTTCCAGATGACAATTATTCTAGTAAACTGCTGTTTTATGTC ATATTCTCTGTGTGCTCTCTGATTAAACTCAATGTACTGAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:4527000 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527000 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_007049 same experiment
	EMAGE:29728 same embryo
	EMAGE:31121 same embryo
	EMAGE:30859 same embryo
	EMAGE:31730 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source