EMAGE mouse gene expression spatial database

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EMAGE:31827

Arpc5l ( MGI:1921442)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001950_01	euxassay_001950_02	euxassay_001950_03	euxassay_001950_04	euxassay_001950_05

euxassay_001950_06	euxassay_001950_07	euxassay_001950_08	euxassay_001950_09	euxassay_001950_10

euxassay_001950_11	euxassay_001950_12	euxassay_001950_13	euxassay_001950_14	euxassay_001950_15

euxassay_001950_16	euxassay_001950_17	euxassay_001950_18	euxassay_001950_19	euxassay_001950_20

euxassay_001950_21	euxassay_001950_22	euxassay_001950_23	euxassay_001950_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 11 16 17

metencephalon basal plate

moderate

regional

moderate expression: see section 08 09 15 16 17

ventral grey horn

moderate

regional

moderate expression: see section 14 weak expression: see section 09 11 13

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 17 18

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 12 13 14 15

facial vii ganglion

moderate

regional

moderate expression: see section 05 18 20

submandibular gland primordium

strong

regional

strong expression: see section 07 08 09 16 17 18

vagus x ganglion

moderate

regional

moderate expression: see section 08 16

thymus primordium

moderate

homogeneous

moderate expression: see section 10 11 13 14

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 17 18 19 20 21 22

vibrissa

moderate

regional

moderate expression: see section 06 07 08 19 20 21

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16 17 18

lower jaw incisor

moderate

regional

moderate expression: see section 10 11 14 15 16

upper jaw incisor

moderate

regional

moderate expression: see section 11 12 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4554
Entity Detected:	Arpc5l, ( MGI:1921442)
Sequence:	sense strand is shown >T4554 CGCCCCCAAGCGGAGCCGGTGCGGAGGGGAGCCGGGAAAGAGCGAAGCCTAGGCGGCTGAGTGGCCGCCG CGCTCCCGCCATGGCCCGGAACACACTGTCCTCACGCTTCCGCCGCGTGGATATCGACGAATTTGACGAG AACAAATTCGTAGACGAGCACGAAGAGGCAGCGGCGGCGGCGGGCGAGCCAGGCCCCGACCCCTGCGAGG TAGACGGGCTCCTGCGGCAAGGGGACATGCTTCGGGCGTTCCATGCAGCTTTGCGGAACTCACCAATCAA CACCAAGAATCAAGCTGTGAAGGAACGAGCCCAGGGTGTAGTCCTGAAAGTACTCACAAACTTTAAGAGC AGCGAAATTGAGCAGGCTGTGCAGTCACTGGACAGAAATGGCATTGATTTGCTAATGAAGTACATTTATA AAGGGTTTGAGAAGCCTACAGAAAATAGCAGTGCAGTGCTACTCCAGTGGCATGAAAAGGCCTTAGCTGT AGGAGGACTAGGCTCCATTATAAGAGTTCTTACAGCAAGAAAGACTGTTTAAAAAAAAATCCTGTTACCT TGAGAAGAATTTTGGATGCCCAGGCTGGTGAAGAAGGGACTGACAATGGACCATCTTCCTAGAACTCCCA ACTAAATACTTCAGGACACATCTTCTGGAATGTATTTTACTTTTGAGGTGGAGGGAGAAATTGTCTTATT GATTCCTAAATTCTTTGCAGGATCAGAATGTCTGCCTTTGTCTACAGAGTTAACACAGAACCAACACTTG ACTATCAGGCTGACTGGCCAGATTATGTCAGGTGTACCTGTGAGCACTGTCTGTTTGAAATGGGGAGGGA CTGTTTGGGCAGGTGCAGATCCACAGGCTGTGGTGAAAAGGAGAGCTTGGGTTCTGAAGCGGAAAAACCT GAGAGTTTGTACAGACATCCTGTCTTCTCAGAGAAGACAGGCTCTCTTGGGTTCATTGTAAAGTGCCTGC TGCATCAATAAAGCTCTTGGCTTATTAGTCTATTCATCGCGGTGTGTTCTCTGTATGTTAAGTGGGAAAA GAAAGGGTTAGTAATGAATGATGATAGTTGGGTTTGGAAAGCTGATGTAGAAGGAGTACAGGTGTCCCTG TCCTGCCATCCCTGGGGGAGGGGGAAAGCAAGCAGGGCGACGACACAGCTGTGCCATATTTCAACTACAG AGGTGCTGGATGCTACCGAGAGACCTTTTGCTGAGTGTTTATTTTTCTTACTCTTTTGATTTGTATGTAA TTTTGGAGCTTATTTAATAAAGTGCCAAACCTTTAATGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4977391 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4977391 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_001950 same experiment
	EMAGE:31518 same embryo
	EMAGE:32192 same embryo
	EMAGE:31460 same embryo
	EMAGE:31721 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source