EMAGE mouse gene expression spatial database

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EMAGE:31936

Fgfbp3 fibroblast growth factor binding protein 3 ( MGI:1919764)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000173_01	euxassay_000173_02	euxassay_000173_03	euxassay_000173_04	euxassay_000173_05

euxassay_000173_06	euxassay_000173_07	euxassay_000173_08	euxassay_000173_09	euxassay_000173_10

euxassay_000173_11	euxassay_000173_12	euxassay_000173_13	euxassay_000173_14	euxassay_000173_15

euxassay_000173_16	euxassay_000173_17	euxassay_000173_18	euxassay_000173_19	euxassay_000173_20

euxassay_000173_21	euxassay_000173_22	euxassay_000173_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

not examined

homogeneous

not examined expression: see section 09

medulla oblongata floor plate

strong

homogeneous

strong expression: see section 10 13

metencephalon basal plate

strong

homogeneous

strong expression: see section 06 08 09 10 11 12 13 14 15 17

ventral grey horn

strong

single cell

strong expression: see section 08 13

medulla oblongata basal plate ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 13 15

basal columns

strong

single cell

strong expression: see section 10

not examined

homogeneous

not examined expression: see section 08 09 10 11

pons ventricular layer

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 15 17

spinal cord

strong

single cell

strong expression: see section 08

cerebral cortex ventricular layer

strong

graded

strong expression: see section 12 moderate expression: see section 02 04 05 06 07 08 09 10 11 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

strong

single cell

strong expression: see section 10 11 12 13 14 15

midbrain floor plate

strong

homogeneous

strong expression: see section 13 not examined expression: see section 08 09 11

medulla oblongata basal plate

strong

homogeneous

strong expression: see section 15

spinal cord floor plate

strong

homogeneous

strong expression: see section 13 not examined expression: see section 10

diencephalon floor plate

strong

homogeneous

strong expression: see section 12

midbrain ventricular layer

strong

homogeneous

strong expression: see section 06 08 09 10 11 12 13 14 15

hypothalamus ventricular layer

moderate

regional

moderate expression: see section 15

corpus striatum

strong

graded

strong expression: see section 02 04 05 06 07 09 19 20 21 moderate expression: see section 08 10 11 13 14 16 17 18 22 23

diencephalon lateral wall ventricular layer

strong

homogeneous

strong expression: see section 10 12 13 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1590
Entity Detected:	Fgfbp3, fibroblast growth factor binding protein 3 ( MGI:1919764)
Sequence:	sense strand is shown >T1590 GCCGAGCTCACTGAGACCTACTGCACCGAGAAGTGGCATTCCCTTTGCAACTTCTTTGTCAACTTCTGGA ATGGCTGAGGCTGCGGGCTGGCAAGGGGCACTAGGGGTAGGTGCTGAGAAGCAGAGGGTAGCGCAGCCGA GGCGGGAGATGTCCTTGGACAGGGAACCGGGATGGAGAATAAGCTAGTCGGCTAGTCGGGATTCCAAAAT GGGTCAGGGGTCAGGGTAAGAAGAATCTGAAGCAGCTGGAGAGGGCTTTTGTTTTTTATTTTATGTTTAT TTTTATTTTTTAAAGCATCTCAGCTCTGATTTAAGAGCAGAAGAAAAAGTGGAAACAAATAGATTTATAT CAAGTATGGGAAAATTGTAGACTAGGGAGAGAAGCTGAGACTAGCAAGAATGAAGTGACTAAAGAGGTGA GAATCTTTCCTAGGAAGTGGGATACAATGTATATCAACACATTTTCATTAAATGTAACTGTGAGTAAGAA CCTGAACCATGTTGAATATTTCAAGATCTTCTTGGTTTT
Notes:	The probe template was PCR amplified from IMAGE:988495 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:988495 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000173 same experiment
	EMAGE:31419 same embryo
	EMAGE:30637 same embryo
	EMAGE:30635 same embryo
	EMAGE:30780 same embryo
	EMAGE:31420 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source