EMAGE mouse gene expression spatial database

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EMAGE:31949

Pdgfrb platelet derived growth factor receptor, beta polypeptide ( MGI:97531)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_004988_01	euxassay_004988_02	euxassay_004988_03	euxassay_004988_04	euxassay_004988_05

euxassay_004988_06	euxassay_004988_07	euxassay_004988_08	euxassay_004988_09	euxassay_004988_10

euxassay_004988_11	euxassay_004988_12	euxassay_004988_13	euxassay_004988_14	euxassay_004988_15

euxassay_004988_16	euxassay_004988_17	euxassay_004988_18	euxassay_004988_19	euxassay_004988_20

euxassay_004988_21	euxassay_004988_22	euxassay_004988_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8040
Entity Detected:	Pdgfrb, platelet derived growth factor receptor, beta polypeptide ( MGI:97531)
Sequence:	sense strand is shown >T8040 AAGAGGTAATCCGGGGCAGAGAAAGCCCACAGTGGTGTGAGCTCTGGGGCTGTCTGTGGCCACAGTCCCG GCTACCCTATCTGGGACCTAGGATTGCTCTGGGAGTAACTTTGAGAGAGAGGAGAACAGAGAGGAAACAG TCCAGAGCCAGAGCGGGCCCAGACCAGTCGTCAGTCTCCTGCCTGCCAGCTAGACCTAGGGCGGCCCCTC GGGCTGCTCCGCTCCTCCCGGAGGATGCTTTTGGAGTGAGGAGGGGCCGGGCTGCTTCTCACCCCTGAGC ACCCTCTCCATTCCCCTGATTCTCTCAGGGTTTTCCGCAATCAGGCCAGCCCTTCTACTGCTGTCCGTTT TTTGGGTCCAGCAAAATAACAGAAGACAGCGAGGTGGACTTCCTGGAGGGGGTGATAGCTCACATCAGAA GCCATCTGTAGCCCGGACACCATGGGGCTTCCAGGAGTGATACCAGCTTTAGTCCTCAGAGGCCAGTTGT TGCTGTCCGTGTTATGGCTCCTGGGACCGCAGACCTCCCGGGGCCTAGTCATCACGCCCCCTGGGCCAGA GTTTGTTCTCAACATCTCCAGCACCTTTGTTCTGACCTGCTCGGGCTCAGCTCCGGTGATGTGGGAACAG ATGTCCCAGGTGCCCTGGCAAGAAGCGGCCATGAATCAGGACGGCACCTTCTCCAGTGTGCTGACACTGA CCAATGTCACTGGGGGAGACACTGGGGAATACTTTTGTGTCTACAACAACTCACTAGGGCCGGAGCTCAG TGAGAGGAAGCGTATCTATATCTTTGTGCCAGATCCCACCATGGACTTCCTCCCTATGGACTCCGAGGAC CTGTTCATTTTTGTCACGGATGTCACTGAGACGACAATTCCGTGCCGAGTGACAGACCCCCAGCTGGAGG TGACGCTACATGAGAAGAAAGTGGATATCCCCCTACACGTACCCTACGACCACCAGCGAGGTTTCACTGG TACTTTTGAGGACAAGACTTACATTTGCAAAACCACCATTGGGGACAGGGAAGTGGACTCCGATACTTAC TACGTCTACAGCCTCCAGGTGTCATCCATCAACGTCTCTGTGAATGCCGTGCAGACTGTGGTCCGCCAGG GCGAGAGCATCACCATCCGGTGCATTGTGATGGGCAATGATGTGGTGAACTTCCAATGGACGTACCCCCG CATGAAGAGTGGGCGGCTGGTGGAGCCAGTGACAGACTACCTCTTTGGAGTGCCCTCCCGCATTGGCTCC ATCCTGCATATCCCCACGGCTGAGCTGAGTGATTCGGGCACCTATACTTGCAACGTGTCAGTGAGTGTGA ACGACCATGGCGATGAGAAAGCCATCAACATCTCTGTGATCGAGAATGGCTACGTGCGGCTGCTGGAGAC ACTGGGAGATGTAGAAATTGCTGAGCTGCACCGGAGTCGGACGCTGCGGGTGGTGTTCGAGGCTTATCCG ATGCCTTCTGTCCTGTGGCTCAAGGACAACCGTACCTTGGGTGACTCCGGCGCTGGCGAGTTAGTTTTGT CTACTCGCAACATGTCTGAGACCCGGTACGTGTCAGAACTGATCCTGGTACGTGTGAAGGTGTCAGAAGC GGGCTACTATACTATGCGAGCCTTCCACGAGGACGATGAGGTCCAGCTCTCCTTCAAGCTGCAGGTCAAT GTCCCCGTCCGTGTGCTGGAGCTGAGTGAGAGTCACCCTGCCAATGGGGAGCAGACAATCCGCTGTCGTG GCCGGGGCATGCCTCAGCCAAATGTCACCTGGTCTACCTGCAGAGACCTCAAAAGTAGGTGTCCACGAAA ACTGTCACCCACACCCTTGGGGAATAGTTCCAAGGAGGAGAGCCAGCTAGAAACGAATGTGACTTTCTGG GAGGAAGATCAGGAATACGAGGTGGTGAGCACACTGCGCCTGCGCCACGTGGATCAGCCACTGTCCGTAC GCTGCATGCTGCAGAACTCCATGGGTGGAGATTCGCAGGAGGTCACCGTGGTCCCACATTCCTTGCCCTT CAAAGTGGTGGTGATCTCAGCCATCCTGGCCTTAGTGGTCCTTACCGTCATCTCTCTCATCATCCTCATC ATGCTGTGGCAGAAGAAGCCACGCTATGAGATCCGATGGAAGGTCATTGAGTCTGTGAGCTCTGACGGTC ATGAGTACATCTACGTGGACCCTGTGCAGTTGCCTTACGACTCCACCTGGGAGCTGCCACGGGACCAGCT TGTTCTGGGACGCACTCTTGGCTCTGGGGCTTTCGGACAGGTGGTGGAGGCCACAGCTCACGGTCTGAGC CATTCGCAGGCCACCATGAAAGTGGCTGTCAAGATGCTGAAATCGACAGCCAGAAGTAGCGAGAAGCAAG CCTTAATGTCCGAGCTGAAGATTATGAGTCATCTTGGACCCCACCTGAACGTGGTCAACCTGCTGGGGGC CTGCACCAAAGGAGGGCCCATCTACATCATCACGGAATACTGCCGATACGGTGATCTGGTGGACTACCTG CACCGGAACAAACACACCTTCTTGCAGCGACACTCCAACAAGCATTGTCCGCCCAGTGCTGAGCTCTACA GCAACGCCCTGCCAGTGGGGTTCTCCCTACCCAGCCACTTGAACCTGACTGGGGAGAGTGACGGTGGCTA CATGGATATGAGCAAGGATGAATCTATAGATTACGTGCCCATGTTGGACATGAAAGGAGACATCAAATAC GCAGACATTGAGTCCCCCAGCTACATGGCCCCTTATGATAACTATGTCCCATCTGCCCCTGAAAGGACCT ATCGCGCCACCTTAATCAACGACTCACCAGTGCTCAGCTACACAGACCTCGTGGGCTTCAGCTACCAAGT GGCCAACGGCATGGACTTCTTAGCCTCTAAGAACTGTGTTCACCGAGACTTGGCGGCCAGGAATGTGCTC ATCTGCGAGGGCAAGCTGGTCAAGATCTGTGACTTCGGCCTGGCTCGAGACATCATGAGGGACTCAAACT ACATCTCCAAAGGCAGCACCTTCCTGCCTCTGAAGTGGATGGCCCCAGAGAGCATCTTCAACAGCCTCTA CACCACTTTGAGTGATGTCTGGTCTTTTGGGATCCTACTCTGGGAGATCTTCACACTGGGTGGCACCCCT TACCCAGAGCTGCCCATGAACGACCAGTTCTACAATGCCATCAAGAGGGGCTACCGCATGGCCCAGCCTG CTCATGCCTCCGACGAGATCTATGAGATCATGCAGAAATGCTGGGAAGAAAAGTTTGAGACTCGACCCCC CTTCTCCCAGCTGGTGCTGCTCCTGGAGAGGCTTCTGGGTGAAGGCTATAAAAAGAAGTACCAGCAGGTA GATGAGGAGTTCCTGAGGAGTGACCATCCTGCCATCCTGAGGTCCCAAGCCCGCTTTCCGGGGATCCACA GCCTCCGATCCCCTCTGGACACCAGCTCTGTTCTCTACACTGCCGTGCAGCCCAATGAGAGTGACAATGA CTACATCATCCCCTTACCTGACCCCAAGCCTGACGTTGCTGATGAAGGTCTCCCAGAGGGGTCCCCCAGC CTTGCCAGTTCCACCTTGAATGAAGTCAACACTTCCTCCACCATCTCCTGCGACAGTCCCCTGGAGCTCC AAGAAGAGCCACAGCAAGCAGAGCCTGAGGCACAACTGGAGCAGCCACAGGATTCAGGCTGCCCAGGACC TCTGGCTGAAGCAGAGGACAGCTTCCTGTAGGAACTGACATCACTCCATTTTGCCCGAATCTCCCTTCTG CCTCCCAGAACCCAACCCTCTTGGCCTGGCCTGGCCTGGCCTGGCCTCCCAGCAGCTACACTGCCACAAG CTGTCCCCTCGAGGAAAGCCCTTGGTTTGCAGCACTCAAGGTCCAGGGACCCAGCTTAGCTTAGGAGGCA AGAGAACTCTGCCTCGGGAAGGTCATGGGACTCTGAACCAGGGTTCCTCCAGGGGACTCAGTTTCCCCAA ATGTAAGAGGAAGAGTTGTACTTGGCTACAGGACAGGTCTGGAGCCCGGATTCCTGCAGAAATCCACGAC TGTACGGTGGTGTGTTCATATCCTCCTGTGTAGCAGCTGCCCCTCAGCTGGATTGCTCTACTTTGATCTT CCTAAGAATCAGGCAAGGACCTGGTGTCTGGCTCCTGGCCAAACTGTAACCAGCCTTGGACAAGGTCTTT TCATTCAGAGCCCACCTCCCCTGGTCTTAGCTTTCCCAGGCCCGAGCTGGTCTGGGGCCAGCCATGCTTA ATGAATGCTGTTAGTGGTGAAGGTAGGCCGAGTACAGAATGCTCTGGCCTGCAGCCCTGCCTGGGCACTC AGGGCACACCTGGCCACAGGAAGCACCCACTCCTTTCAGCCCCACCAGTCCTAGAATAGTCCCCAGGTCA CTCTCAGCTGACCCACCCACCAGAGTCTGCAGGGCCATTGTCCACGCCTGTGATAGGCTAAGCATCTGCC TGAAGTGTGTACCTACCACTAGGAGCCCTGGCTCTGCGCTGGACCTGCTATGAGACCTTGGGGGCTTTCC TTGTTCTCTCTGGGGACCAGTTTTCTTTCCCCTTTGAAAAGCAAGTTGGACACATAGACTCTGAGTACCC TGTCAATAGTACAATTCTTGTATGTTCTGGGAGTTTGCTCTTGTCCCGAGGAAGCAGGGTCAAGTCCTTA AACTGATCTTTCTAGGGGTCAGCTGAGCTCTTGGAAGATCCCTGATACACTTCACACTCTGGGGGTTCAG GAACCGAGCCTCTTCTTCAAGTCTCCAAGTGCAACTGCCCAGACCTTGACTTGGAGTGACAGTGAGTGTC CTAGGAAACCCCCTTACAGCTGTCCTGAGGACACAGAAGAGACCACGAGACCCTTTTTTTCATATCATGA AGCCAGCAAGAGTGGCAGAGAAGGCAAGCCAGGTTACCTCGGGCCACTGTCACCAGCAGCGTGTGGACAG ACATGATGGACAGTGAGGACAGACGTCCCATGCAGGACAAGAGACTTGAGATGGGCAATGAGAGGAAGAT GCTGACGGGATTACCACCTCAACTGCCAGCTGCCCCCGTCCCCAGGGAGCACCCCACAGAAACAGTTCTA ACCCTGAACCAATGAACATTGCAAGTGCCTGGGGACTAGGGAGTGGGGGGAAGTCAGCCTTTCTGGGGAC CCTCCCTGCTAGCTGGTTGGCTAGCTGGCATCTCCCCGCTTGGAGCAGCAGAGGCTGGGGAGTACTGCTC ACAATGGTACCAAAGATAGAATCACCTAGGTTTACAAGTACTTGTAGGACTCGAGATAACCCACATTTAG ACACCGGAAGTGCTATTTTATATGCTGTTAAGTTTTCCTATCTGTACTTTTTTTTTAAATGGGAAAGATT TTAATATTAAACTTGGTGCTTCTCACTGAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30060666 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30060666 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004988 same experiment
	EMAGE:30638 same embryo
	EMAGE:32011 same embryo
	EMAGE:31058 same embryo
	EMAGE:31650 same embryo
	EMAGE:31973 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source