EMAGE mouse gene expression spatial database

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EMAGE:31962

Slc24a3 ( MGI:2137513)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005602_01	euxassay_005602_02	euxassay_005602_03	euxassay_005602_04	euxassay_005602_05

euxassay_005602_06	euxassay_005602_07	euxassay_005602_08	euxassay_005602_09	euxassay_005602_10

euxassay_005602_11	euxassay_005602_12	euxassay_005602_13	euxassay_005602_14	euxassay_005602_15

euxassay_005602_16	euxassay_005602_17	euxassay_005602_18	euxassay_005602_19	euxassay_005602_20

euxassay_005602_21	euxassay_005602_22	euxassay_005602_23	euxassay_005602_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 08 09 10 11 12 13 16 21 22 23 24 weak expression: see section 06 07 14 15 17 18 19 20

basal columns

moderate

regional

moderate expression: see section 08 09 10 11 12 13 16 17 weak expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4389
Entity Detected:	Slc24a3, ( MGI:2137513)
Sequence:	sense strand is shown >T4389 CCCGTGTGGGCCCTTGCTGGTGTTGGGCAAGACTCTGCTGGCAAGAACTTGACCTCATGGATCTCATTGG GGAAGATAGAAAATGGATGGTGGGGAGGAAGCTGATGCAGGTGAATGATACACTGACTTCTGAAGATGCA GGGCTCCAGAGCAGCAAGAACTGCACAGAACCAGCCCTGCATGAATTCCCCAGGGACATCTTCTCCAATG AAGACCGACGACAGGGAGCAGTGGTCCTCCACGTGCTCTGTGCCATGTACATGTTCTATGCGCTGGCCAT TGTGTGCGATGACTTCTTCGTCCCATCCTTGGAAAAGATCTGTGAGCGCCTCCACCTCAGTGAAGATGTG GCAGGAGCCACATTCATGGCAGCAGGCAGCTCGGCCCCAGAGCTGTTCACGTCGGTCATAGGAGTCTTCA TCACAAAGGGTGATGTGGGAGTTGGCACCATCGTGGGCTCTGCCGTGTTCAACATCCTGTGCATCATTGG CGTCTGCGGGCTCTTTGCAGGGCAGGTGGTGGCTCTTTCCTCCTGGTGCCTGCTGAGGGACTCCATCTAC TACACGCTGTCTGTGGTCGCACTCATTGTGTTCATTTACGATGAAAAAGTTTCTTGGTGGGAATCTCTAG TCCTTGTGCTGATGTACCTCATCTATATCGTCATCATGAAATACAATGCGTGTATACACCAGTGCTTCGA AAGGAGGACGAAAGGTGCCGGGAACATGGTCAACGGATTGGCCAACAACGCAGAAATCGACGACAGCAGC AACTGCGATGCCACTGTGGTGCTACTCAAGAAAGCCAATTTCCACCGCAAAGCGTCCGTGATCATGGTGG ATGAGCTGCTGTCTGCCTACCCACACCAGCTTTCTTTCTCTGAGGCTGGCCTTCGAATTATGATCACAAG CCACTTTCCTCCCAAGACCCGCCTCTCCATGGCCAGTCGCATGTTAATCAATGAGAGACAAAGACTGATA AACAGCAGGGCCTATACCAATGGGGAATCTGAAGTGGCCATCAAAATCCCAATTAAGCACACAGTGGAGA ACGGAACAGGGCCCAGCAGTGCCCCAGACAGGGGTGTGAACGGAACCCGGAGGGACGACATAGTTGCTGA GACTGACAATGAGACAGAGAACGAGAATGAAGACAATGAGAATAACGAGAATGATGAAGAGGAGGAGGAG GATGAAGATGACGATGAAGGCCCCTACACGCCGTTCGACCCCCCCTCTGGCAAACTGGAAACTGTGAAAT GGGCTTTCACCTGGCCACTGAGCTTTGTCTTGTACTTCACTGTCCCCAACTGTAACAAACCCCACTGGGA GAAGTGGTTTATGGTGACATTCGCTTCCTCTACGCTATGGATCGCAGCCTTCTCCTACATGATGGTGTGG ATGGTTACCATCATCGGCTACACACTAGGGATCCCTGATGTCATCATGGGGATCACCTTCCTGGCTGCCG GAACCAGCGTGCCAGACTGCATGGCCAGCCTCATTGTAGCCAGACAAGGGATGGGGGATATGGCTGTGTC CAATTCCATTGGGAGCAACGTCTTTGACATCTTGATTGGGCTCGGTCTTCCCTGGGCTCTGCAGACTCTG GCTGTAGACTACGGATCCTATATTCGGCTGAATAGCAGAGGGCTCATCTACTCCGTGGGCTTGCTCCTGG CCTCTGTCTTTGTCACGGTCTTTGGTGTTCACTTGAACAAGTGGCAGCTGGACAAGAAGCTGGGCTGTGG ATGCCTCTTCCTGTACGGCGTGTTCCTCTGTTTCTCCATCATGACGGAGTTCAACGTGTTCACCTTTGTG AACTTGCCCATGTGTGGGGACTAATGATTGGCCAGGCCACCTGCTGAGGCCCAGGTCCTTCTTTTCTGTG CAATACGAGACCCGGCCGCACCCGAGTCAGGCGGCCCCTCAACAGCCGAGGCATCCGTCTCTCCCGTGCT GTCCACAGGCCTCCGCTCCTGTCCTGGTGGCCTAGGCTCTCCCTGCCACCTCCTTTGCTCCCCAACTCCC TCGGCCCTACCTCCCAGCCGGCCCCCCTCACCTGCCCACCCCATTCCTGCCTCCTCTTCCGTGTGAAGAC ATCCAACATCCACATGAATTTTCAAGCTCCATTTTTGAACAGTGACTGAGATTCTAGAACAAAACTGGCT GCTAACTGGCCCAAGCCAGGCACAACTTACTCCAATCCCTCCTTCTTTTTTAAGTTATTTCACAGAATAC CCTAATTTATGACCAGAGACTTTGAGGAAACAGGAGAGGGTCCGCTGATGGCAGGAAGGTTTGTTTTGTT TGGGAGTGAGTCCTAGGTTACGGGGCTCAGGGAAATTGTTTAATTTGAGGGGGCGCTTTTGTTTCTTTAC TTTTCTTTCAGAGACAGAGGAATCCCCTCGCCGTCTTCTCTCACTGGGCTCTGGCTTAATAGCTGATCCA CAGATTGTACTGAGTGTCTCCTGGGGGTGAATCCCAGGCACCGATGGGAATTTAATAATTAGTTGGGAAA CCACTGAGCAAGACCACCAGGAATAAATGCATGACATGACATTTTCTGCCCAAGGGACACATCACTCAAG TCCTGAAGGCTTCACCTGGGGGTGGGGGGCTGTAAGGTGTTAGAGGAAGAAACCTGCTTCACACATGTTC ACTATGACCATCTCTCTCCCACTAGGTTCTTTCTAGTTTTTCAAGCAATAGCTCTCTCCTGCCTAAGGCC TTTGACAGGGAGGCCCCGGCTACCTGTCCATAGGGACAGCAGGCACCAGGCAGGCAACGACAGAAACTTT CCCAGCGTGTGAGTCCTTGAAATGTGCTCTTGTTGCTTGGCATTTGTGGTGACAGGGAATGACCCCAGAG GCCGTCAACTGTGCTCTGGACAGAAGTTACAGACACTGGTTTCTTGGAAAATGAAGAGAAAGCACATTTT GCACAGATGTCGTCAATGAAGTCCCAATTTGCCACTTGGTATCGAGTACACTGGACCCTGACAGCTGGCT CTTGAGCAAGCATCCTTCCTTACAGGATGCCACCAACAATGCCACCAACAAGCTAGCCAGTTTCACCCCT CCCCTCCAGGAGGGATGGCCAGGGAGGGAGAAAGCAGAGAACTGCTGTGTTGAAAACCACAGAATGGGTT CACCTCAGACTGGCTCAAGGGCACAAGGTATTGTGAACATTTCGCCAATCACTGCTCAACCGCCCTGCTA GATTTTCTATGACGCTGAATTATTATGCAGAATAACCCCACCAAGTTGAGATACACCCATGCTTGTTACA CTTGTATTTATTGAAACTGTGGATTCTTGCCCGCCCGTGCTCTCCCTTGTATTTACTTTAAGCACTGATC ACTTATCATCCATTCGGTATGGTTTTCCCAGTCCCTTGTACACATCCTGGTTTGAATTGTAAAAAAAAAA AAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4914291 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4914291 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005602 same experiment
	EMAGE:30923 same embryo
	EMAGE:29251 same embryo
	EMAGE:29256 same embryo
	EMAGE:29796 same embryo
	EMAGE:31963 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source