EMAGE mouse gene expression spatial database

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EMAGE:31963

Rdh10 retinol dehydrogenase 10 (all-trans) ( MGI:1924238)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_005601_01	euxassay_005601_02	euxassay_005601_03	euxassay_005601_04	euxassay_005601_05

euxassay_005601_06	euxassay_005601_07	euxassay_005601_08	euxassay_005601_09	euxassay_005601_10

euxassay_005601_11	euxassay_005601_12	euxassay_005601_13	euxassay_005601_14	euxassay_005601_15

euxassay_005601_16	euxassay_005601_17	euxassay_005601_18	euxassay_005601_19	euxassay_005601_20

euxassay_005601_21	euxassay_005601_22	euxassay_005601_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

mesothelium

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16

stomach

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11

spinal cord

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17

rectum

moderate

regional

moderate expression: see section 14 15 16

brain

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midgut

moderate

regional

moderate expression: see section 12 13 14 15 16 17 18 19 20 21 22

bladder

moderate

regional

moderate expression: see section 12 13 14 15

foot

strong

regional

strong expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 09 10 16 17 18 19 moderate expression: see section 11 12

genital tubercle of male

moderate

regional

moderate expression: see section 15 16

naris

strong

regional

strong expression: see section 16

hand

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23

lower lip

strong

regional

strong expression: see section 11 12 13 14 15 16

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4416
Entity Detected:	Rdh10, retinol dehydrogenase 10 (all-trans) ( MGI:1924238)
Sequence:	sense strand is shown >T4416 CTCGGGGGCGGACTCCGGGCGCAGCCTTCGCGTCCGGATCGCGGTGACAAGCGCCTCCGGGGCCAGGAGC TGGGCTGTCTGGCGCAGGGAGAGTGAGGACACGGTGCTGGGCTGCTGCGGGACCCGGGGCGTCGCGATGA ACATCGTAGTGGAGTTCTTCGTGGTCACTTTCAAAGTGCTGTGGGCGTTCGTGCTGGCCGCGGCGCGCTG GCTGGTGCGGCCCAAGGAGAAGAGCGTGGCCGGCCAGGTGTGCCTCATCACGGGCGCGGGCAGCGGCCTG GGCCGCCTCTTTGCTCTCGAGTTCGCCCGGCGCCGGGCGCTGCTGGTTCTCTGGGACATCAACACGCAGA GCAATGAGGAGACCGCAGGCATGGTTCGCCACATCTACCGGGACCTGGAGGCGGCCGACGCTGCTGCTCT GCAAGCTGGAAAAGGTGAGGAGGAAATCCTGCCCCCGTGTAACTTACAGGTTTTTACCTACACTTGTGAC GTGGGGAAGAGAGAGAATGTCTACCTGACAGCTGAAAGGGTCCGCAAGGAGGTTGGCGAGGTCTCGGTCC TGGTCAATAATGCTGGTGTGGTTTCTGGACATCACCTTCTGGAATGTCCTGATGAGCTCATTGAAAGAAC CATGATGGTCAACTGCCACGCACACTTCTGGACCACTAAGGCCTTTCTTCCAACGATGCTAGAGATTAAT CATGGCCACATTGTGACGGTCGCGAGCTCCCTGGGACTGTTCAGCACTGCTGGAGTTGAGGATTACTGTG CCAGTAAATTTGGAGTTGTGGGTTTTCACGAGTCTCTGAGCCATGAGCTAAAGGCTGCTGAAAAGGATGG AATTAAAACAACTCTGGTTTGCCCTTACCTTGTAGACACGGGCATGTTCAGAGGCTGCCGAATCAGAAAA GAAATTGAGCCCTTTTTGCCCCCCTTGAAGCCTGATTACTGTGTGAAGCAGGCTATGAGGGCCATCCTCA CTGACCAGCCCATGGTCTGCACGCCCCGCCTCATGTACATCGTGACATTCATGAAGAGCATCCTTCCTTT TGAAGCCGTCGTGTGCATGTATCGATTTCTAGGAGCGGACAAGTGTATGTATCCCTTTATTGCTCAAAGA AAACAAGCCACAAACAACAATGAAGCAAAAAATGGAATCTAAGAGTGTTTTTGTACGGAGTGCCGTTTCT ATCAGGATAGGTGGTCCGTGCGCATCAATCAGTACTGACGGTCTGGATTCTAACCCCATGCCGACTCTTT TTTTTTTTTTTCTAATCAACTTTTTAAAAAATAAAGTGTAAATTAACTGACTAGTGTGCTTGAAAACTGT GAGCTTGGGTTTTTGCCAATGGGGTCTTTTCTAGACAGGGCCCTAAAAATGAGTCCAATATTTAGACAAA CACTATGTTACATCATTATTTTTTTAATGAACATAAAGTCTAGAAATGATGACTACAGTGTGTTTCACGT GGGTCCCCACAGAATTGATTCATTCATTATCGTGGAAGCCCAGCCAGCATTCAAGGACCGACAGTGTAGT GCTCTGTTGTGTTCTCGGTTCTTCTCCTGCAAGCCTGGGTGGCTTGCTTATGGTGTCTGTGGATTTTTTG ATGTCAGTTTGAGATCAGCGTAGTCTGAGCATCACAATGAAGGCCACCAGTCTCTTTCTTAAACTTACAG TGACTAAATATTGGGTGATTTTCAAATTCAGTCTCTGAAATACTAAATTAAATGTTTAGAGACCTGGTTA ATCCACGTTTGTACATTTTCTACCTGTGAATCTTGCTGCATATAGAAAGTGCCCTTTCCTCACGGAGCTG CAAACTTGTGTTAGGGGAGGAAGTGGGGGATCTTGTCTACAGGACACAGCAGCTCCACAGAGATAATGGG AATTTCTGATTAAAAAGTCCCCGTACTTGTGCCAACTTTAATTAGCTTGGGGAGCAAATCTTATATGGAG TGTTGTGGAAGGGTGGAAAGATTTCTTTGAAAAGTTTATTCACATTGTAGAACAGCAAATGACATTTTTA CAGTATTTTTTTGTAAAGCAAACTATTTTGTGCCTTGAATTTGGTATATGTGTATTAGTGAAACATTGTA AAAGTGAATTTCTACCTCTGTATCTATGTGTACCATCCTCTTGTAAATTACTATGAACTACTATGTGATT GCTTCTGTTTGTTCGTTCTAGGATGTCTTTTTTAAATAGTGACCAATGTTTAAGGCTGTTAAAATAAGCC AACTTTTACAAATCGGGACGTTTTCTAAAGAACTGATTAAATACGTACATAACTGAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4923005 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4923005 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_005601 same experiment
	EMAGE:30923 same embryo
	EMAGE:29251 same embryo
	EMAGE:29256 same embryo
	EMAGE:29796 same embryo
	EMAGE:31962 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source