EMAGE mouse gene expression spatial database

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EMAGE:31979

Camk2g calcium/calmodulin-dependent protein kinase II gamma ( MGI:88259)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_012590_01	euxassay_012590_02	euxassay_012590_03	euxassay_012590_04	euxassay_012590_05

euxassay_012590_06	euxassay_012590_07	euxassay_012590_08	euxassay_012590_09	euxassay_012590_10

euxassay_012590_11	euxassay_012590_12	euxassay_012590_13	euxassay_012590_14	euxassay_012590_15

euxassay_012590_16	euxassay_012590_17	euxassay_012590_18	euxassay_012590_19

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 11 12 13

facial vii ganglion

moderate

regional

moderate expression: see section 02 15 16 17

vagus x ganglion

moderate

regional

moderate expression: see section 13 14

trigeminal v ganglion

strong

regional

strong expression: see section 01 moderate expression: see section 02 03 04 05 14 15 16 17 18 19

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 07 08 09 11 12 13 14 15 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 04 15

spinal cord

weak

spotted

weak expression: see section 05 06 07 08 09 10 11 12

brain

weak

spotted

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 04 13 14

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31563
Entity Detected:	Camk2g, calcium/calmodulin-dependent protein kinase II gamma ( MGI:88259)
Sequence:	sense strand is shown >T31563 ACCTTCGCAGCCAGTGACTCTGGAGGGCCTGAGTGACAGCGGCGGTCCTGTTCGTTTGAGGTTTAAAACA ATTCAATTACAAAGCGGCAGCAGCCAATGCACGCCCCTGCATGCAGCCCTCCCGCCCGCCCTTCGTGTCT GTCTCTGCTGTACCGAGGTGTTTTTTACATTTAAGAAAAAAAAAAAAAGAAAATAAGATTGTTTAAAAAA ACGGAATTCATACCATGATGCGTTTTAAAACAACTGATAGCCCTTGGCTTGCAAGGAGACAGGAGTTTGT GTGATGCTCATCCAGGCATGAACAGTGGGCTCACCCACCAGTATTGAAGAGGTGAGCTTGGCCTCAGGCC CCCATGGATTCAGGGGGACCAGGCAAGGACTCTTGACAGAGCTTTGGTCATGGACAAAGCCATGGTGTGG TTGCAGCCCCAGAGGTGGCCTGATCACCCCAGTTCTAGGAGTGGACTTCGTCAGACCATGCATTGACACC TAGTATGAGAAAGACGCAAACACCGTGGCCGCTGGACCTACATTGGAGAGAATGCAGCACTGCCAGCCTG CTGTGTGTGGAGGCAGCTGTGAGGCGTGGCCTCAGGACTGAAAGCCTGGACTTTGCTGTACCAGTCCAGT TAGCGTAGACAGGAAGAGATTTGGTGCTGCAGAAGTGTGTCCGCCAATGAGAAACCTCGAGTTCGTCTGA CATGCACTTAACTCATCCATTTCTAGAGGATGCACAATGCACGTGGGCCTGAGACTGAGGCCCGATTTCT GCTGGTGGAAAGGGGGAGGGATTGCTACTTTGTTTCCTGTTGGTTTTCCTGACATAACAAGGAGAGATCC AGAAGTCGTTGGCCAGGGCTCCTTTTGCCACTTTTGGCAGTTGTTTGTGTGCTGATCTGGACCATCTTTG TCTTGTATTTTCATGGTGGTGGTCCCCATGCTGACCCTCAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5342641), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 31466. Forward Primer - name:031466_F_IRAV71-74_A01_Camk2g, sequence:ACCTTCGCAGCCAGTGAC; Reverse Primer - name:031466_R_SP6_IRAV71-74_A01_Camk2g, sequence:GATGAGGGTCAGCATGG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_012590 same experiment
	EMAGE:32007 same embryo
	EMAGE:30561 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source