EMAGE mouse gene expression spatial database

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EMAGE:32044

Gdnf glial cell line derived neurotrophic factor ( MGI:107430)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_000342_22	euxassay_000342_01	euxassay_000342_02	euxassay_000342_03	euxassay_000342_04

euxassay_000342_05	euxassay_000342_06	euxassay_000342_07	euxassay_000342_08	euxassay_000342_09

euxassay_000342_10	euxassay_000342_11	euxassay_000342_12	euxassay_000342_13	euxassay_000342_14

euxassay_000342_15	euxassay_000342_16	euxassay_000342_17	euxassay_000342_18	euxassay_000342_19

euxassay_000342_20	euxassay_000342_21	euxassay_000342_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

gut

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 moderate expression: see section 04 weak expression: see section 17

duodenum rostral part vascular element

strong

spotted

strong expression: see section 03

duodenum rostral part epithelium

strong

spotted

strong expression: see section 03

metanephros

strong

regional

strong expression: see section 09 10 11 12 17 18 19 20 21

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3862
Entity Detected:	Gdnf, glial cell line derived neurotrophic factor ( MGI:107430)
Sequence:	sense strand is shown >T3862 TGGCCTCGAGCCAGATTCGGACGAGGGTAGAAGTTAATGTATCACTCCCTTTGTGGCTGCTATCCCCCGC CCCCAGAGTAGGACAGATAGATCTAAAATAATAAATGACCAATTAATTGGCCTCTCTCGAATAGTCATGT CAAATTTTCAAAGTAACCAAGAGGTAAAAGTTACTAGGTATCCTTTCCCCTTCCGTGGCCCTAAAGACCC ACGTTTCGCATGGTTCCACACCCTCTGCAGGACTGGGAAGTGGAGCTACGTATGCGCTGCTCTCTCCGCT CCTCCTGCTCAAAATTGTGACAACCTCAGCTGCCCAGCACATTTCAGATGTCGTTCCAGACCCTCTGTTC ACTCCAGAGAATCCTGAAAGATGTGGTAGCTGATGCTCCCAGGTCCAGACACAGCCTCTAGCTCTTGGGG GAATCCCTGGAGAACTCAGCATCTCGAGCAGGTTCGAATGGGAGCAGGCTGCCCCACCCAGTCCGCAGCA GGGTAAAGTTTGCGACGGTTCTTTACTGTTGTGATCAGCATCTGCCCCAGTAAAGAG
Notes:	The probe template was PCR amplified from IMAGE:425671 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template usi. T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:425671 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_000342 same experiment
	EMAGE:29705 same embryo
	EMAGE:29707 same embryo
	EMAGE:30058 same embryo
	EMAGE:32018 same embryo
	EMAGE:29667 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source