EMAGE mouse gene expression spatial database

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EMAGE:32105

Smpd1 sphingomyelin phosphodiesterase 1, acid lysosomal ( MGI:98325)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_006555_01	euxassay_006555_02	euxassay_006555_03	euxassay_006555_04	euxassay_006555_05

euxassay_006555_06	euxassay_006555_07	euxassay_006555_08	euxassay_006555_09	euxassay_006555_10

euxassay_006555_11	euxassay_006555_12	euxassay_006555_13	euxassay_006555_14	euxassay_006555_15

euxassay_006555_16	euxassay_006555_17	euxassay_006555_18	euxassay_006555_19	euxassay_006555_20

euxassay_006555_21	euxassay_006555_22	euxassay_006555_23	euxassay_006555_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

choroid invagination

strong

regional

strong expression: see section 08 09 10 11 12 13 17 18 19 20 21

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6849
Entity Detected:	Smpd1, sphingomyelin phosphodiesterase 1, acid lysosomal ( MGI:98325)
Sequence:	sense strand is shown >T6849 CGAAGGGTAATCAGGTGTCTCCAGCGTTACCCGGGCCCCGAGGGCCAGCCAGGGCTTCGGCCTGTGGGGC CTGGAGGGGCAAGCCAGTCCTGTCTACGAGCCTGGCAATGCCCCACCACAGAGCATCATCCGGCCAGGAC CACCTCAGAGCCGGCTGGGAGCAGAGACTGGAGAGGTCCTTACCGGCACCCAGAGTGGGACTCCTTTGGA TGGGGCTGGGCTTGGCGCTGGTTCTGGCTCTGTTTGACACCACGGTTCTTTGGGTTCCTGCCAGAGCTTA TCCTCTTCCTTCTGAAGGCCATTCTGTCAAATTCAGTGCCATAGCGCCGCCGCTCCAGAGTGCCTTTGGG TGGCAGAACCTCACTTGCCCCGCCTGCAAAGTCTTATTCACTGCTCTCAACCATGGGCTGAAGAAGGAGC CCAATGTGGCACGGGTAGGCTCTGTGGCCATCAAGATATGCAAGATGCTGAACATAGCACCACTAGATGT GTGCCAGTCAGCCGTCCATCTCTTTGAGGACGATGTGGTGGAGGTGTGGACACGTTCAGTTCTGAGCCCA TCAGAGGCTTGTGGCTTGCTTTTGGGCTCCTCTTGTGGACACTGGGACATCTTTTCGACTTGGAACATCT CTTTGCCATCAGTGCCGAAGCCACCCCCAAAGCCACCGAGCCCACCAGCCCCCGGTGCCCCTGTCAGCCG TGTCCTCTTCCTTACTGATCTACACTGGGACCATGAGTACCTGGAGGGCACAGACCCTTACTGTGCAGAT CCACTTTGCTGCCGCCGGGGGTCCGGATGGCCACCCAACTCCCAGAAGGGGGCTGGGTTCTGGGGCGAGT ACAGCAAGTGTGACCTGCCCTTGCGAACGCTAGAAAGCCTGTTGAAAGGACTGGGCCCTGCCGGCCCTTT TGAAATGGTGTACTGGACGGGAGATATCCCTGCCCATGATGTCTGGCAACAGTCTCGCCAAGATCAGCTG AGGGCCCTGACCACCATCACAGACCTCGTGAGGAAGTTCTTGGGCCCAGTGCCAGTGTACCCTGCTGTGG GCAACCATGAGAGCACTCCTGTCAATGGCTTCCCTCCTCCCTTCATAAAGGGAAACCAATCTTCACAATG GCTCTATGAAGCCATGGCCAAGGCATGGGAACCCTGGTTACCAGCTGATGCCCTTCACACCCTAAGAATT GGGGGCTTCTATGCCCTCACCCCACGCCCTGGCCTCCGCCTCATCTCTCTCAATATGAATTTTTGTTCCC GTGAGAACTTTTGGCTCTTGATCAACTCCACAGATCCTGCTGGACAACTCCAGTGGCTGGTGGAGGAGCT TCAGGCTGCTGAGAATCGAGGAGACAAAGTGCATATAATTGGCCACATCCCTCCAGGACATTGTCTTAAG AGCTGGAGCTGGAATTATTACAAAATCATAGCCAGGTATGAAAACACTCTGGCCAGTCAGTTCTTTGGCC ACACTCACGTGGATGAGTTTGAGATCTTCTATGATGAGGAAACTCTGAGCCGACCACTAGCTGTAGCCTT CCTGGCGCCCAGTGCTACAACCTTTATCAACCTTAACCCTGGCTACCGAGTTTACCAAATAGATGGAAAC TACCCCGGAAGCTCTCATGTGGTCCTGGACCACGAGACCTACATCTTGAATCTGACCCAGGCCAACGCAG CAGGAGGCACACCAAGCTGGAAGCGCCTCTACAGGGCTCGAGAAACCTATGGACTTCCAGATGCGATGCC TGCCTCCTGGCACAACCTGGTCTACCGCATGAGGGATGATGAGCAACTCTTCCAGACCTTCTGGTTTCTC TACCATAAGGGCCACCCACCTTCAGAGCCCTGCGGCACACCCTGCCGCCTGGCCACTCTGTGTGCCCAGC TCTCAGCCCGAGCAGACAGCCCTGCTTTGTGTCGCCACTTGATGCCCAATGGGAGCCTCCCAGATGCCAA TAGGTTGTGGTCACGCCCCCTGTTGTGCTAGTATTCCATGTTCCAGACTGGGGAAAGTTCATGTATCAGA AAAGGGATACATTCCCGAGTGCTGCTTATCTACCTGAGGCAAAAGCTTCGGGGGAAGGAGCCAGCCAGGC CAGGGAAACAAGCCGCCTCATGAGAGCTTGTTTGGTTACACTGGAGGAGGTTTTGGCTGTCCTACTTATG CCCAGGATCTGGATTACCCCTGGGGCTGATGTCTGACCTGGGCTTTGTCGAGGGCTCAGTGCTCTGGATA GTCAGACAGGAGCTATTGCCCGAGCCTGGGCTAATGGACCTCTGAACCCTGTACTGTTACCTGATCCTGC AAGGCTGTTAAAATAAAGAGACACACTTCAGACTCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3964394 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3964394 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006555 same experiment
	EMAGE:30668 same embryo
	EMAGE:29628 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source