EMAGE mouse gene expression spatial database

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EMAGE:32136

Ppm1a ( MGI:99878)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003492_01	euxassay_003492_02	euxassay_003492_03	euxassay_003492_04	euxassay_003492_05

euxassay_003492_06	euxassay_003492_07	euxassay_003492_08	euxassay_003492_09	euxassay_003492_10

euxassay_003492_11	euxassay_003492_12	euxassay_003492_13	euxassay_003492_14	euxassay_003492_15

euxassay_003492_16	euxassay_003492_17	euxassay_003492_18	euxassay_003492_19	euxassay_003492_20

euxassay_003492_21	euxassay_003492_22	euxassay_003492_23	euxassay_003492_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 14 17 18 19 20

facial vii ganglion

strong

regional

strong expression: see section 04 moderate expression: see section 05 06 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 09 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 moderate expression: see section 05 06 07 08 09 18 19 20 21 22 23

vibrissa

moderate

regional

moderate expression: see section 06 07 08 09 20 21 22 23

cervical ganglion

moderate

regional

moderate expression: see section 10 18

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 08 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 11 12 18

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

thoracic ganglion

moderate

regional

moderate expression: see section 15 16

testis

moderate

regional

moderate expression: see section 07 08 09 10 22 23 24

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6271
Entity Detected:	Ppm1a, ( MGI:99878)
Sequence:	sense strand is shown >T6271 GGGACCCGCCCGCCCGCGGCTGCTCCGGACCTAGAGGATCAAGTCATAATGGGAGCATTTTTAGACAAGC CAAAGATGGAGAAGCATAATGCCCAGGGGCAGGGGAATGGGTTACGATACGGCCTAAGCAGCATGCAAGG TTGGCGAGTTGAAATGGAGGACGCACACACGGCTGTGATCGGTTTGCCAAGTGGACTTGAGACATGGTCA TTCTTTGCTGTATATGATGGGCATGCTGGTTCTCAGGTTGCCAAATACTGCTGTGAGCACTTGTTAGATC ACATCACCAATAACCAGGATTTCAGAGGATCTGCAGGAGCACCTTCTGTGGAGAACGTAAAGAATGGAAT CAGAACAGGGTTTCTGGAGATTGATGAACACATGAGAGTTATGTCAGAGAAGAAACATGGTGCAGATAGA AGCGGGTCAACAGCTGTGGGCGTCTTAATCTCTCCCCAACATACTTATTTCATTAACTGTGGAGACTCGA GAGGTTTACTTTGTAGGAATAGAAAAGTTCACTTCTTCACACAAGACCATAAACCAAGTAACCCGCTGGA AAAAGAACGAATTCAGAATGCAGGGGGCTCGGTGATGATTCAGCGTGTCAATGGCTCTCTGGCTGTATCG AGGGCCCTTGGGGATTTCGATTACAAATGTGTCCATGGAAAAGGTCCCACAGAGCAGCTTGTCTCCCCAG AGCCCGAAGTCCATGATATTGAAAGGTCTGAAGAAGATGACCAGTTCATCATCCTTGCATGCGATGGCAT CTGGGACGTCATGGGGAACGAAGAGCTCTGTGACTTTGTGAGATCCAGACTTGAAGTCACTGATGACCTT GAGAAAGTTTGCAATGAAGTAGTCGACACCTGCTTGTATAAGGGAAGTCGAGACAACATGAGTGTGATTT TGATCTGTTTTCCAAGTGCACCCAAAGTCTCGGCAGAGGCGGTGAAGAAGGAGGCGGAGCTGGACAAGTA CCTGGAGAGCAGAGTAGAAGAAATCATAAAGAAGCAGGTGGAAGGCGTCCCTGACTTAGTCCACGTGATG CGCACGTTAGCCAGTGAGAACATCCCCAGCCTCCCACCAGGGGGTGAATTGGCAAGCAAGCGGAATGTAA TTGAAGCCGTTTACAATAGACTGAACCCTTACAAAAATGACGACACTGATTCTGCGTCAACCGATGATAT GTGGTAAAGCCGCTCACCCAGCCGTGGACTCACCTTCGCCTGCAAAGGGGAAGCCAGCTCATCCTTGCCG AGCCTTTACCATCCATCACCGACTTCACAGGAGGGTCTGACACGGGTGAGGACTGCAGCTTCAGCGATGG AACAGCTAGCCCAGAATGGATTTTCTTTTTTTATTGTAAATTTGAGACTTATGTAAGCGTGATTTCAAAC CGTAATTCATGTTGTAAATCAGACTCCAGCAATTTTTGTTGTATGATTTTGGTTTTTGTAAAGTGTAATT GTCCTTGTACAAAGTGCTCATATTTAATTATGAACTGCTTTAAAATCACTATCAGTGAATGATAAAGGAA ATTTGGCTTGTTGTGTGACACAACAGACATAGTCATGCTGTGTCTGGACTCGGTTGGGATAGGGAAAGCG GCAGCCACATAGCTGCATGCTACGTGCACACGATTAGGAAGAATTCCAAAAGCTTACACAGCTGTCACCC AGGCTCATGGAAACTGTCTCTGTTCCGGCGAGGACTGGCTCACTGATACAGCCATCGCTCTCACCTGTCT CTAGGATGTCCTCTCCTCGTAGCCAAGATATTGCAGCTGATCCTGTATATTCATGAGAGTATCTATCAAT TTTGGGCTAACATGCCTTGATTCTCTTGCACCTCTTTATGAGTTCTTACATTTAATTACTAATTGGTAAG CAGCAGCTTCCTACACAGTAGGAGACTGCCACATTTTTTTCCTATCATGACTGGCTGGGCCTGCTGCTGT TCCTAGTAAGATATTCCGAATTCCATTTTATCAATAAAGCTTGGTTTAACAAACAAGAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:3581897 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3581897 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003492 same experiment
	EMAGE:32130 same embryo
	EMAGE:32177 same embryo
	EMAGE:32129 same embryo
	EMAGE:31331 same embryo
	EMAGE:31931 same embryo
	EMAGE:29768 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source