EMAGE mouse gene expression spatial database

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EMAGE:32141

Il28ra ( MGI:2429859)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003252_01	euxassay_003252_02	euxassay_003252_03	euxassay_003252_04	euxassay_003252_05

euxassay_003252_06	euxassay_003252_07	euxassay_003252_08	euxassay_003252_09	euxassay_003252_10

euxassay_003252_11	euxassay_003252_12	euxassay_003252_13	euxassay_003252_14	euxassay_003252_15

euxassay_003252_16	euxassay_003252_17	euxassay_003252_18	euxassay_003252_19	euxassay_003252_20

euxassay_003252_21	euxassay_003252_22	euxassay_003252_23	euxassay_003252_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 17 18 19 20

pharyngo-tympanic tube

moderate

regional

moderate expression: see section 01 02 03 04 23 24

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 19 20 weak expression: see section 10 11 12 13 14 15 16 17 18

hindgut

weak

regional

weak expression: see section 14 15 16 17 18 19 20

epidermis

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

naris

weak

regional

weak expression: see section 14 18

foregut-midgut junction

weak

regional

weak expression: see section 17

stomach

moderate

regional

moderate expression: see section 02 03 04 05 06

rectum

weak

regional

weak expression: see section 15

urethra of male

weak

regional

weak expression: see section 14 15

midgut

weak

regional

weak expression: see section 11 12 13 14 15 16 17 18 19 20 21

bladder

weak

regional

weak expression: see section 14 15

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4719
Entity Detected:	Il28ra, ( MGI:2429859)
Sequence:	sense strand is shown >T4719 GGGACCCCAGGGACCGAAATGTGGCGGGCCGACCGGTGGGCGCCCCTACTCCTGTTCCTGTTGCAGAGCG CCCTAGGAAGGCCCCGTCTAGCCCCACCCAGAAACGTGACACTCTTCTCCCAGAACTTCACTGTTTACCT GACATGGCTTCCGGGGCTTGGGAGCCCCCCAAATGTGACCTATTTCGTGACCTACCAAAGCTATATCAAA ACCGGTTGGCGACCAGTGGAGCATTGTGCAGGTATCAAGGCTCTGGTGTGTCCCCTGATGTGCCTGAAGA AACTGAACCTGTACTCCAAGTTCAAAGGACGAGTACAGGCAGCTTCCGCACAGGGCAGGTCCCCACGGGT GGAGTCCCGGTACCTGGAATACCTTTTTGACGTGGAGCTAGCCCCGCCCACCCTGGTGCTCACCCAGATG GAGAAGATCCTAAGGGTCAATGCTACCTACCAGCTGCCACCTTGCATGCCGTCGCTGGAACTGAAGTACC AGGTGGAGTTCTGGAAGGAGGGTCTGGGAAGCAAGACCCTGTTTCCTGACACTCCCTATGGCCAGCCAGT GCAGATTCCTCTCCAGCAAGGTGCTAGCCGACGCCACTGCCTCAGCGCCAGAACCGTCTATACCTTAATT GACATTAAGTACAGCCAGTTCTCTGAGCCCAGCTGCATCTTCCTAGAGGCTCCAGGGGACAAAAGAGCTG TCCTGGCATTGCCCTCACTCTTGCTTCTACTGATAGCAGCCGTTGCAGCAGGTGTGGCATGGAAGATAAT GAAAGGAAACCCCTGGTTTCAGGGGGTGAAGACGCCCCGGGCACTGGACTTTTCTGAATACAGATACCCA GTGGCAACCTTTCAGCCCAGTGGACCTGAGTTCTCTGATGACTTGATCCTTTGTCCCCAGAAGGAACTGA CCATAAGGAACAGGCCAGCCCCTCAGGTCAGAAACCCAGCCACGCTACAGGCAGGACCAGAAAGAGACAG TACTGAGGATGAAGACGGGGACACAGACTACGATGACGACGGTGACAGCGTCCAGCCCTACCTGGAACGG CCCCTCTTCATCAGCGAGAAGCCCCGGGTTATGGAACACTCGGAGACAGACGAGTCTGGGGTGGATTCAG GGGGGCCTTGGACTTCCCCAGTTGGGAGTGACGGCTCCTCTGCATGGGACTCTTCAGACAGGAGCTGGTC CAGCACAGGGGACTCCTCATATAAGGATGAAGTTGGGTCTTCCAGCTGTTTGGACCGAAAGGAGCCGGAC CAGGCGCCCTGTGGGGATTGGCTCCAGGAGGCTCTCCCATGCCTGGAATTTTCTGAGGACTTGGGCACCG TGGAAGAGCCTCTGAAGGATGGCCTCTCCGGGTGGAGGATTTCTGGTTCCTTATCCTCAAAGAGAGATCT GGCTCCTGTGGAGCCCCCAGTTTCTCTTCAGACACTGACTTTCTGCTGGGTCAACAATCCTGAGGGGGAG GAGGAACAGGAGGACGAGGAGGAAGAGGAGGAGGAAGAGGAGGAGGAAGACTGGGAATCAGAACCTAAGG GCAGCAATGCCGGCTGTTGGGGCACTTCAAGCGTGCAGAGGACGGAGGTCAGGGGCCGGATGCTGGGAGA TTACTTGGTCAGGTGAGCTGGCCCTGGTGGTCCATCTACACATTCTCTACTGCACTTCCCCTGGACCACA GACACCTCCGAGAGTCATGTCCCTTTGAGTGGTAGCCAAGGTCCTGGCCATTCGTTATGGATCTTTATGG CACTTTGTCGTTGGGATTGAGTTGTAAGATGTCACCATTTCCAATGACCCACCAGCTATAGGTGACCTGT GAGCGGGACGGAGCTGTGATCAGAGACTAGTTGAGTTCCAGTCTGCCCATTGTCCCTGGGATGACGGGCC TGATGGAAAAATCATCCTGTGATGAAATGAAACAGGTGAAGCCAGGTGGCTGCGGACACCAAGGGTAGAT ACGCCCTAGTCCATAAGTCACACACAGCATTGCGCAAAACAGACCTCGGTCCGAATCACTACTCTGCTGT TCCTGGTAGTCACAGAGCAAGCGACTCTTAACCTCTCAACCTCAGCTTCCCCTTCTGCAGCAGTCCTGTG GGTATGGGGAGGTAACAGGTGACACAGGGCCACCAATGTCACATGTACTGACTAGGCACTAGAACCCTCC ATCAGTCCAGACTCCTGGGAGACCCCAGGCATCTCTCCCCAGCAACCCCTAAGCCAAGAGTGATAAGACA TCGACCTTCCAACTTGTCTGCCCTCTAGCTCACCCCATTTCTTCCTTGCCACCTTCTATTCAGGGGCTCC TGGAAACCACAGCCCAACCAACTTTGCTTCGTTCTCAAGCATAGTACACCTTCCAGGGTCGACATTGGTT GGTTCAGGGCAGAAACTGCACTCCTGGCCTGGTGTCCTTTTTGAATGGCATTGTATCAGGGTTTAAGTTT TTTTGGGTATGTTTGTTTGTTTGTTTGTTTGTTTTTGTCTGTTTTGGCCATAATGAGGTTCCAGTATCTG AGAAGCTAGTGTCTTTTGAAAACAGGTGACTGTCCTTTTTGGGGTGCGATGTGTGTCCTTGTGGCTGAGG ATCTAAACAGACCATGAAGCAGGAAAGAGCTCACAGCCCCTTTACTCTCGGAGCTGTGTGGCTCTGAGGC TGGCATAAAGCCATAGCCTTACAGAACTCATGTGAAGACCCCCCCCCCCGTAAGGCAGAGGTTCTCAACC TTCCTAACACTACCAGCCTTAAACAGTGTTCCTTGTGGTGTGGTGACCCCCAATCATAAAATCATTTCAT TGCTACTTCATAATTATAATTTTGCTATTGGTATGAATCACAATGTGTTTTCCAATGGTCTCAGGCAACC ACTGTGAAAACGTCAATGGACCCCCAAAGGGGTCATGACCCACACAGTCTGAGAAACATGACCTTAAGGG CATCTGACTCAAGGGTTCTCTAGCTCATTCCCAAAGGCCTTTGGGGCTCCAGGATCCTCACTCTTCAATG GGAGTGGCCTCACTCAGCTAATGCACATGTAGTGGCTTTACATAAAAGGTGTCTTGAAAGGGGTTCACAG CTAACAAAACCTCAAATCACTGTCCTTGGTCCATATCTGTATTGTTTCAAACAAACTTGGATGTCAACTA GCAAGGCTGCCCTAGTGACTTCATTTGTGTGTCCCCAAGAGTCCCCAGAGTCACCAATCTGACAGGAGGT CTTTGCAAGCACCTCTGGGACACACACACACACACACACACACACACACCTACCTCTCTGGGGTTTCCCT ACAATGATGTTTGACCACATCACTGAGCCCAGAAGCAGAACAAATGCACATGCGTGAGGGCGGTCAGAGC GCGGGAGAAGCCCCTAGAAATGTCCCAGCAAGGCCAGTGTCCTTCCTGAGACTCAGCTGAGCTGCACCAC GGAGGATGCTCTGTCAGGAATCCTTGGAAATGATGGTGATGACAGCATTGGCCTCGCCTTTCAAGCTTGC TCTCTCCGGTAGTCCTTACTGTTTACAGTGTTTCCCAATGTTTTCTTTCTGATCTAGCCTTCCTGTCATT CTTAATCCTAGCTCCTTTCAGCCATTAGGTCATTGACAGAAGACCTGTGGGAAGCCTGTGGAAAGGGCGG GAGGTGCAACCCAGAAGTTCATAATGTGTGCTCAGCACTTAACTTACATAATAGCCCTGTACACAGACAG CCTTCGGGGACCTCAAGGAGAGCAAGATCAGAGTGACCGCCTGGAGTCAGTGTGGTGGGCAGCTTCGGCA CACTGAGGCTATACGTCTTACGTGTTTGTATTACCTGTCTTGCATGTTTGTATTTTTGTCGAAATCGTGC CCTGTATTTTATACCCTATTTAAGTAATACTTTAATATCTGTTGGTATAATACCCAAAACCTTAAATAAA GAGATTTATTTTTAGAGAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5036481 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5036481 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003252 same experiment
	EMAGE:32180 same embryo
	EMAGE:30744 same embryo
	EMAGE:32189 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source