EMAGE mouse gene expression spatial database

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EMAGE:32180

Srfbp1 ( MGI:1914472)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_003230_01	euxassay_003230_02	euxassay_003230_03	euxassay_003230_04	euxassay_003230_05

euxassay_003230_06	euxassay_003230_07	euxassay_003230_08	euxassay_003230_09	euxassay_003230_10

euxassay_003230_11	euxassay_003230_12	euxassay_003230_13	euxassay_003230_14	euxassay_003230_15

euxassay_003230_16	euxassay_003230_17	euxassay_003230_18	euxassay_003230_19	euxassay_003230_20

euxassay_003230_21	euxassay_003230_22	euxassay_003230_23	euxassay_003230_24

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 11 18 19 20

renal cortex

moderate

regional

moderate expression: see section 07

renal capsule

moderate

regional

moderate expression: see section 07 08 09 10 11 weak expression: see section 17 18 19 20 21

orbito-sphenoid

moderate

regional

moderate expression: see section 06 07 22 weak expression: see section 03 04 05 23 24

testis

moderate

regional

moderate expression: see section 21 22 weak expression: see section 06 07 08

axial musculature

weak

regional

weak expression: see section 04 05 06 19 20 21 22

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4647
Entity Detected:	Srfbp1, ( MGI:1914472)
Sequence:	sense strand is shown >T4647 GGAGATGGCGGCTGACCCTCTTCCTCCGTCCGCAATGGTTCAGCCGGGGACTCTGAACCTCAATAACGAG GTTGTAAAGATGAGAAAAGAAGTGAAGCGAATCCGAGTTCTGGTCATCCGAAAACTTGTCAGGAGTGTCG GCAGACTGAAGTCCAAAAAGGGCACAGAAGATGCACTGTTGAAAAACCAAAGGCGAGCCCAAAGGTTGCT TGAGGAGATCCATGCCATGAAGGAATTGAAACCTGATGTTGTAACTAAATCTGCTCTTAGTGATGATATC AACTTTGAAAAAACTTGCAAAAAGCCGGATTCTACCGCGACCGACAGAGCAGTTGCCAGATTAGCAGGTC ACCCGCTTCTGAAGAAGAAAATTGATGTGCTAAAAGCTGCTGTCCAGGCCTTCAAAGATGCAAGGCAGAG TGCTCCAGCGGCCGAGTCATCTGAGAGTACTTCAGGAGAAGGCCGGTATAAGGACATTGCAAGGTCAAAG GATGATGCAAGTGAGTCACAGCATCCTGAGAGAACTGTTGTCAGCGAGCAGAAGGCAAAGGACACCAACA CGGCAGCGAAGAATGCAGGAAGTGGTTCAAAAGAAAAACTGGCCAAGACAGAGCAGGCACCCAGAGCAGG GACCACTCCAGGGTCTCAGGGGAAGCCTTCAGGAAAGGGTGCTGGGGTTAACTCTGAACACCAGGGGGCC CCAGCCCCAGGGGACAGCAACCAGGGGAAAGCCTCAACCAAAACCCCGGCGGACAGCGTGTGTGAACCTG CTAATAACGGTGTCAGTGAGGAAGAGGAGAGCGAGGGGGAAAAGGAGTATTTTGATGACAGCACTGAAGA ACGGTTCTACAAACAGTCTTCTGCATCTGAGGACAGCGACAGTGGTGACGACTTCTTCATTGGAAAAGTC AGACGGACTCGAAAGAAGGAATGTGGTGTCCATTCCTCGGCCAAGGAACTGAAGCCCCTCCCAAAGGTAC CATCTAAAACAAGTACACTTGAAACCCCCTGGGATGTAAGAAACGATAAACACAGGCCAATTCCAGAAGC CAGGAAGTTCGAATCCGTGTTTTTCCACTCGTTGGCTGGACCTAAAAGCTCCAGAAGGGATCCCAGAGAA CAGGCCCCAAAGAATAAAGCCCCAGATTTTCCAGAAAATGAACCTCCAGTCAAGAAGCAGTTTACAAAGA GTGCACACAGAGGCTTTGAGAGTGTGAAGCAGCCGATGCAGGCACCTCTGCATCCGTCCTGGGAAGCGAG CAGGAGGCGGAAGGAGCAGCAGTCCAAAATCGCTGTGTTTCAGGGGAAAAAAATTACATTTGATGATTGA TTTTGGTACTTGCTGCCTCTTTGCAAGCGTTCTGTCAAAATTTTTGGTTTTGTTTTTAACTAACTTGTTA TTTAAACTTGCATTTTAAATAAGTCTCTTTCAAAGAGTTATAAAATATATTGCTTTTTTCTCTTTTTTTA TGTGAATTTAGGGAATATTTACATAATCTTTTTACAGATCTCTAAATTTGTTTCATAAGAATGCTTGCCC CAAATTATTTCTTACATAAAGTAATTTAGATATTACGGAATTTTAGGTTTTCTCATTCATGATTACAAAA TATATTTCGCTAGTTAAGCTTTCCCTTTTGCCTTCAAAATAAGGCAAACAGGGCTTAGGAGATGGCTCAC TTCCTGAGGCCCGATGGCCTGAGTTTGACCCCTGTGATTCCCTTGGTATGAGGGCAGAACTAACTCACAA AGTCGTTCTCTGATTGCACATACAGTGTGGCACATGCCTATGTACACACACATATGTTCACATATAGTAA ATGTCATATAAAAATTTTAGGACATATTTAAAATAATAGTGAGAAAAGAAAGAAATTAGGACTAAAAAAT ATTTTTATAAGCAAAACAGAAATGGCAATTTTCATTGTGGATATAGAAATAGTATATAAATAAATATTAA TTTGTGATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5009612 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZ. T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5009612 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_003230 same experiment
	EMAGE:32141 same embryo
	EMAGE:30744 same embryo
	EMAGE:32189 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source