EMAGE mouse gene expression spatial database

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EMAGE:32231

Slc12a6 ( MGI:2135960)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_016520_01	euxassay_016520_02	euxassay_016520_03	euxassay_016520_04	euxassay_016520_05

euxassay_016520_06	euxassay_016520_07	euxassay_016520_08	euxassay_016520_09	euxassay_016520_10

euxassay_016520_11	euxassay_016520_12	euxassay_016520_13	euxassay_016520_14	euxassay_016520_15

euxassay_016520_16	euxassay_016520_17	euxassay_016520_18	euxassay_016520_19	euxassay_016520_20

euxassay_016520_21	euxassay_016520_22	euxassay_016520_23

View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

vomeronasal organ

weak

regional

weak expression: see section 13

telencephalon ventricular layer

weak

regional

weak expression: see section 10 11 12 17 18 19 20 21

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 14 15 16 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38730
Entity Detected:	Slc12a6, ( MGI:2135960)
Sequence:	sense strand is shown >T38730 TTTCTGGTATACATTGTCCCCCGAGCTGCCATCTTTCGGAGTGACGATGCACTCAAGGAGTCAGCAGCTA TGCTGAACAACATGCGCGTCTATGGTACAGCCTTCTTGGTCCTCATGGTCTTGGTGGTATTCATCGGCGT ACGCTATGTGAATAAGTTTGCCTCACTCTTCCTGGCCTGTGTAATTGTGTCGATCTTGGCTATCTATGCT GGTGCCATCAAGTCTTCCTTTGCTCCACCACACTTCCCGGTCTGTATGCTGGGCAACCGTACCCTGTCAT CAAGACACCTTGACATTTGCTCTAAGACCAAGGAGGTTGACAACATGACAGTACCATCAAAGTTATGGGG ATTCTTCTGCAACTCGAGTCAGTTCTTTAATGCCACCTGTGATGAGTACTTTGTTCACAATAACGTCATC TCAATCCAAGGCATTCCAGGGTTGGCTAGTGGTATCATTACTGAAAATCTTTGGAGTAATTATTTACCAA AGGGTGAGATAATTGAAAAGCCATCAGCCAAGTCATCTGATGTCTTAGGCAACTTAAACCATGAATATGT TCTTGCTGATATCACCACCTCCTTCACTCTGCTGGTGGGGATCTTCTTTCCCTCGGTCACAGGTATCATG GCTGGGTCAAACAGATCTGGAGATCTGAAAGATGCCCAGAAGTCTATTCCCATTGGGACCATCCTTGCCA TCCTGACCACATCCTTTGTGTATT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 103482. Forward Primer - name:103482_F_cDNA_Slc12a6, sequence:TTTCTGGTATACATTGTCCCCC; Reverse Primer - name:103482_N_SP6_cDNA_Slc12a6, sequence:AATACACAAAGGATGTGGTCAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_016520 same experiment
	EMAGE:32238 same embryo
	EMAGE:32228 same embryo
	EMAGE:32229 same embryo
	EMAGE:31920 same embryo
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source