EMAGE mouse gene expression spatial database

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EMAGE:32244

Tnni3 troponin I, cardiac 3 ( MGI:98783)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


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View assay images:
Expression pattern clarity:

Expression Pattern Description

Text Annotation:

Structure

Level

Pattern

Notes

heart atrium

strong

regional

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

heart ventricle

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9973
Entity Detected:	Tnni3, troponin I, cardiac 3 ( MGI:98783)
Sequence:	sense strand is shown >T9973 AGTGTCCTCAGTGAGGCTTGAGCAGCCCAGAGGAAACCCAACCTCTAGAGACCTCCAAGGTCACCAGGGA CACCCTTCCAGGACCCTCCAGGAATCTCCGATCCTGTTCTCTGCCTCTGGAGATCATCATGGCTGATGAG AGCAGCGATGCGGCTGGGGAACCGCAGCCTGCGCCTGCTCCTGTCCGACGCCGCTCCTCTGCCAACTACC GAGCCTATGCCACCGAGCCACACGCCAAGAAAAAGTCTAAGATCTCCGCCTCCAGAAAACTTCAGTTGAA GACTCTGATGCTGCAGATTGCGAAGCAGGAGATGGAACGAGAGGCAGAAGAGCGACGTGGAGAGAAGGGG CGCGTTCTGAGGACTCGTTGCCAGCCTTTGGAGTTGGATGGGCTGGGCTTTGAAGAGCTTCAGGACTTAT GCCGACAGCTTCACGCTCGGGTGGACAAAGTGGATGAAGAGAGATATGACGTGGAAGCAAAAGTCACCAA GAACATCACTGAGATTGCAGATCTGACCCAGAAGATCTATGACCTCCGTGGCAAGTTTAAGCGGCCCACC CTCCGAAGAGTGAGGATCTCTGCAGATGCCATGATGCAGGCGCTGCTGGGGACCCGGGCCAAGGAATCCT TGGACCTGAGGGCCCACCTCAAGCAGGTGAAGAAGGAGGACATTGAGAAGGAAAACCGGGAGGTGGGAGA CTGGCGCAAGAATATCGATGCACTGAGTGGCATGGAAGGCCGCAAGAAAAAGTTTGAGGGCTGAGCCATG GCTCCCACACTGTGCTCTGAAGGACGTCCCTGCAGAATAAACCTCTCCAAACCACAAAAAAAAAAAAAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30300830 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template usi. T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30300830 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_006970 same experiment
	EMAGE:32199 same embryo
	EMAGE:29576 same embryo
	EMAGE:32211 same embryo
	EMAGE:29933 same embryo
	EMAGE:29932 same embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source