EMAGE mouse gene expression spatial database

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EMAGE:3336

Gsx2 GS homeobox 2 ( MGI:95843)
TS18 (36-38 Somite no.)
immunohistochemistry

Data Images


Figure 2A of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 2B of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 2C of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 2D of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 2E of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Figure 2F of Corbin et al., 2003 [PMID:12930780] . Copyright: This image is from Corbin et al., Development 130, 4895-4906 (2003), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
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Notes:

***Note: these sections are double stained for GSH2 (green) and NKX2-1 (red). Regions of overlap in expression appear yellow. Only the data for GSH2 has been annotated in this EMAGE entry.*** The sections are coronal telencephalic sections shown from anterior (A) to posterior (F). Image annotations: CGE, presumptive future caudal ganglionic eminence; Di, diencephalon; Sep, septum.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

3336_voxel_moderate_3D_1.wlz

3336_voxel_possible_3D_1.wlz

3336_voxel_notDetected_3D_1.wlz

3336_voxel_strong_3D_1.wlz

3336_voxel_moderate_3D_2.wlz

3336_voxel_possible_3D_2.wlz

3336_voxel_notDetected_3D_2.wlz

3336_voxel_strong_3D_2.wlz

3336_voxel_moderate_3D_3.wlz

3336_voxel_possible_3D_3.wlz

3336_voxel_notDetected_3D_3.wlz

3336_voxel_strong_3D_3.wlz

3336_voxel_moderate_3D_4.wlz

3336_voxel_possible_3D_4.wlz

3336_voxel_notDetected_3D_4.wlz

3336_voxel_strong_3D_4.wlz

3336_voxel_moderate_3D_5.wlz

3336_voxel_possible_3D_5.wlz

3336_voxel_notDetected_3D_5.wlz

3336_voxel_strong_3D_5.wlz

3336_voxel_moderate_3D_6.wlz

3336_voxel_possible_3D_6.wlz

3336_voxel_notDetected_3D_6.wlz

3336_voxel_strong_3D_6.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3336_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon

detected

regional

A. In anterior telencephalon, expressed in lateral domain. B. At the level of the septum, expression is non-overapping with Nkx2.1 expression. C. More posteriorly, some Gsh2-positive cells observed within the Nkx2.1 domain. D. More posteriorly, some Gsh2-positive cells observed within the Nkx2.1 domain. E. More posteriorly, some Gsh2-positive cells observed within the Nkx2.1 domain. F. At the most posterior level of telencephalon, highly expressed in the region of the presumptive caudal ganglionic eminence.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	antibody
Identifier:	MGI:2150169
Entity Detected:	Gsx2, GS homeobox 2 ( MGI:95843)
Antigen:	sense strand is shown >MGI:2150169 ANEDKEISPL
	aa 296 - aa 305 of P31316
Notes:	The antibody used in this study by Corbin et al., 2003 [PMID:12930780] was a "rabbit anti-Gsh2 (1:2000, gift of K. Campbell, Cincinnati, OH, USA)". This antibody was raised against the C-terminal peptide, ANEDKEISPL (Hsieh-Li et al., 1995 [PMID:7619729] )". See also Figure 1 in Hsieh-Li et al. The secondary antibody was either a FITC-conjugated donkey anti-rabbit or Cy3-conjugated donkey anti-rabbit (both from Jackson ImmunoResearch, West Grove, PA, USA).
Antibody Type:	polyclonal
Raised In:	rabbit
Supplier:	K.Campbell, Cincinnati.

Specimen

Organism:	mouse
Age:	36-38 Somite no.
Theiler Stage:	TS18
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	4% paraformaldehyde
Embedding:	cryosection
Secondary Antibody:	donkey anti-rabbit
Labelled with:	Cy3 or FITC
Visualisation method:	fluorescence

General Information

Authors:	Corbin JG; Rutlin M; Gaiano N; Fishell G, 2003. [PMID:12930780] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1242/dev.00717] [ PMID:12930780] Corbin JG, Rutlin M, Gaiano N, Fishell G 2003 Combinatorial function of the homeodomain proteins Nkx2.1 and Gsh2 in ventral telencephalic patterning. Development (130):4895-906
	[ doi:10.1016/0925-4773(94)00334-J] [ PMID:7619729] Hsieh-Li HM, Witte DP, Szucsik JC, Weinstein M, Li H, Potter SS 1995 Gsh-2, a murine homeobox gene expressed in the developing brain. Mech Dev (50):177-86
Links:	MGI:2676238 same experiment
	EMAGE:3435 same sections & embryo
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source