EMAGE mouse gene expression spatial database

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EMAGE:4689

Bves blood vessel epicardial substance ( MGI:1346013)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Fig5A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9751] Dev Biol 223(2): 371-82, Andree B; Hillemann T; Kessler-Icekson G; Schmitt-John T; Jockusch H; Arnold HH; Brand T, Isolation and characterization of the novel popeye gene family expressed in skeletal muscle and heart. Copyright 2000 [PMID:10882522]	Fig5A'. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9751] Dev Biol 223(2): 371-82, Andree B; Hillemann T; Kessler-Icekson G; Schmitt-John T; Jockusch H; Arnold HH; Brand T, Isolation and characterization of the novel popeye gene family expressed in skeletal muscle and heart. Copyright 2000 [PMID:10882522]

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotations: sv - sinus venosus; ve - right ventricle; arrowhead - expression in the dorsal aspect of the right ventricle. Fig5A' is a section taken from the embryo shown in fig5A at the level indicated by the black line.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4689_wholemount_moderate_3D_1.wlz

4689_wholemount_weak_3D_1.wlz

4689_wholemount_notDetected_3D_1.wlz

4689_wholemount_strong_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4689_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

heart atrium

detected

Pop1 was expressed in the atria and dorsal half of the ventricle. It was confined to the sinoatrial compartment and some restricted areas in the dorsal part of the ventricle.

primitive ventricle

detected

regional

Pop1 was expressed in the atria and dorsal half of the ventricle. It was confined to the sinoatrial compartment and some restricted areas in the dorsal part of the ventricle.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	Bves probeA
Entity Detected:	Bves, blood vessel epicardial substance ( MGI:1346013)
Notes:	The Bves (popeye1/Pop1) probe used in this study by Schmitt-John et al., 2000 [PMID:10882522] is described as "a 400-bp EcoRI 5' fragment of mouse Pop1." Editors Note: EcoRI digestion of the mouse Bves cDNA RefSeq NM_024285.2 (1607nt) predicts two sites at nt134 and nt738. It is unclear from the description of Schmitt-John et al., which 400bp region was used as probe (however one of the EcoRI sites is presumed to be linker derived).
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	ICR
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Andree B; Hillemann T; Kessler-Icekson G; Schmitt-John T; Jockusch H; Arnold HH; Brand T, 2000 [PMID:10882522] , Indexed and Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.2000.9751] [ PMID:10882522] Andrée B, Hillemann T, Kessler-Icekson G, Schmitt-John T, Jockusch H, Arnold HH, Brand T 2000 Isolation and characterization of the novel popeye gene family expressed in skeletal muscle and heart. Dev Biol (223):371-82
Links:	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source