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EMAGE:5220

Wnt6 wingless-related MMTV integration site 6 ( MGI:98960)
TS19 (43 Somite no.)
in situ hybridisation

Data Images
EMAGE:5220 EMAGE:5220
Fig2A. Copyright: This image is from [doi:10.1002/dvdy.10441] Veltmaat JM; Van Veelen W; Thiery JP; Bellusci S, "Identification of the mammary line in mouse by Wnt10b expression." Dev Dyn 2004 Feb;229(2):349-56. Reprinted with permission of Wiley-Liss Inc. [PMID:14745960] . Fig2A. Copyright: This image is from [doi:10.1002/dvdy.10441] Veltmaat JM; Van Veelen W; Thiery JP; Bellusci S, "Identification of the mammary line in mouse by Wnt10b expression." Dev Dyn 2004 Feb;229(2):349-56. Reprinted with permission of Wiley-Liss Inc. [PMID:14745960] .

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Lateral view of Wnt6 expression in whole embryo (A), with higher magnifications of the flank of the embryos (A'). fl: forelimb; hl: hindlimb; s: somite stage; 3: 3rd mammary rudiment.
Expression Pattern Description
Spatial Annotation:
EMAGE:5220Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
5220_wholemount__moderate.wlz
5220_wholemount__weak.wlz
5220_wholemount__possible.wlz
5220_wholemount__notDetected.wlz
5220_wholemount__strong.wlz
(what is wlz format?)
Download all expression domains: EMAGE:5220_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
surface ectoderm
detected detected
regionalAt the 43-somite stage, Wnt6 expression is detected at a low level almost ubiquitously in the surface ectoderm and the underlying dermis and slightly higher in the dorsal part of the embryo (Fig. 2A). At this stage, the developing placode 3 highly expresses Wnt6, while expression is only slightly elevated at the mammary line itself and seems to follow the progression toward the limbs in a manner similar to Wnt10b expression. Similar to Wnt10b but with differences in timing and pattern, Wnt6 expression is fragmented along the flank aligned with the somites has become fainter. Of interest, Wnt6 expression is restricted to the placode (number 3) at the 43-somite stage when Wnt10b expression is less defined as a fragmented streak.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt6 probeB
Entity Detected:Wnt6, wingless-related MMTV integration site 6 ( MGI:98960)
Notes:The Wnt6 probe template used in this study by Veltmaat et al., 2004 [PMID:14745960] was "pBS-SK-Wnt6 (full-length Wnt6)", "kindly provided by Dr. A. McMahon".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:43 Somite no.
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Veltmaat JM; Van Veelen W; Thiery JP; Bellusci S. 2004 [PMID:14745960] . Indexed and spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1002/dvdy.10441] [ PMID:14745960] Veltmaat JM, Van Veelen W, Thiery JP, Bellusci S 2004 Identification of the mammary line in mouse by Wnt10b expression. Dev Dyn (229):349-56
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE