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EMAGE:6159

Wnt10a wingless related MMTV integration site 10a ( MGI:108071)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6159 EMAGE:6159 EMAGE:6159 EMAGE:6159 EMAGE:6159
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:6159Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
6159_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6159_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt10a probeA
Entity Detected:Wnt10a, wingless related MMTV integration site 10a ( MGI:108071)
Sequence:sense strand is shown

>Wnt10a probeA
GGGGGGTTGCGCCCCAGAACTTCGGCCTCCAGTCCCCAGCCCGCTGCACCTCCTTACCCTCTAGAGGCCC
CCTCCCCCTTACCCTCTAGAGGCACCAGGAGTTGTCGCAAGGGGCCCTTGGGAAATTCCCTGGACCCCTG
TGCCAGGAGGTGCCCGGTTCGCCCGCTCCCCATCCACCCCCCCGAGGGCGGTGCCCGGGGGCGCTGCCCC
ATGGAGCGGGGAGGCGGGCGCCGTCTGCTGCGGGAGCTGTGACCTGAGTAGGAGCTGTGTGTCGCAGCCG
CCCCACCCCTGCCGATCATGCGCCGGCGACCCTGGTTCGCCAGTCCCACTGGGCTGTGAGCCCCCCACTC
CTGGCCTGTCACGGCCCGCGCGCCATGGGCAGCGCCCACCCTCGCCCCTGGCTGCGGCTCCCACAAGGGC
CCCAGCCGCGGCCTGAGTTCTGGGCGCTCCTGTTCTTCCTACTGCTGCTGGCTGCCGCTGTGCCCAGGTC
AGCACCCAACGACATCCTGGGCCTCCGCCTACCCCCAGAGCCCGTGCTCAACGCCAACACAGTGTGCCTG
ACATTGCCCGGCCTGAGCCGGCGGCAGATGGAGGTGTGTGTGCGTCACCCTGACGTGGCCGCCTCTGCTA
TCCAGGGCATCCAGATCGCCATCCATGAGTGCCAGCATCAGTTCCGGGACCAGCGCTGGAACTGCTCCAG
CCTGGAGACTCGGAACAAAGTCCCCTACGAGAGCCCCATCTTCAGCCGAGGTTTTCGAGAGAGTGCTTTC
GCCTACGCCATAGCAGCTGCCGGGGTGGTGCACGCAGTGTCCAACGCGTGCGCTCTGGGTAAACTGAAGG
CTTGCGGTTGCGACGCCTCCAGACGTGGGGACGAAGAAGCTTTCCGTCGGAAGCTGCACCGCTTGCAGCT
GGACGCGCTGCAGCGCGGAAAGGGCTTGAGCCACGGGGTCCCTGAACACCCGGCCATACTTCCTGCCAGC
CCAGGTCTGCAGGACTCCTGGGAGTGGGGTGGCTGCAGTCCGGATGTGGGCTTCGGAGAACGCTTCTCTA
AGGACTTTCTGGACTCCCGAGAGCCTCACAGAGACATCCATGCTCGAATGAGACTCCACAACAACCGTGT
GGGCCGGCAGGCGGTGATGGAGAACATGCGGCGTAAGTGCAAATGCCACGGCACCTCAGGCAGCTGCCAG
CTCAAGACCTGCTGGCAGGTGACGCCTGAGTTCCGCACAGTAGGGGCGCTGCTGCGCAACCGCTTCCACC
GCGCCACGCTCATCCGGCCGCACAACCGCAACGGTGGCCAGCTGGAGCCCGGCCCCGCGGGAGCACCCTC
GCCAGCACCGGGCACTCCAGGGCTGCGCCGCAGGGCCAGCCACTCCGACCTGGTCTACTTTGAGAAATCT
CCCGACTTCTGTGAGCGCGAGCCGCGCCTGGACTCGGCAGGCACTGTGGGCCGCCTGTGCAATAAGAGCA
GCACGGGTCCCGATGGCTGCGGCAGCATGTGCTGTGGCCGCGGCCACAACATTCTGCGCCAGACGCGCAG
CGAGCGCTGCCACTGCCGGTTCCACTGGTGCTGCTTCGTGGTCTGCGAAGAATGCCGCATCACCGAGTGG
GTCAGCGTCTGCAAGTGAGCAGACCCAAGCTCCTCTGGGTCTCAAGAATGGTTGTCCTCTTGGTGCCTGG
CTTCTGCCGCTAGCGGATCTGAGCCAGGCAGCAAGCAGCAGCCTTGGCTCCTGAGAGAGGTGGTTGGCTC
TTACAGCCCCGAGGGTCTACAATCACCAGACAGTCCAGATCTGATTGACATTCCTCCGCTCACCTCTGTA
GGTTCCCCTCTTTCTGTTCCTAGCTCAGACAGCTGGGGGTGATAGTGGAGACTGTTCCACACCCTAGGAC
AGGTCACCAAAGCAGCCCAGCCTGGCATGCCTACCTCCTGTCATCTCTTCTTCCCTTCCCCAGGAGTGAT
AGGCAATGCACTGAAGCTGATGGGCACCGGGGAAGAAAACTAAAAGGCAGAAATGGCCGTCATCGGGCTG
AAGTGACTCTAAGGGCTCCAGACCTCTGCTCCTGTCTTTCACTTAACAGATATTTATTTTTGCGCTCTCT
TTGAGACACTCTCTGGGGAAAAAGAAGCTCCGGAGTCTACAGGCTGATTAAGGGACATGGACAATAAACC
AGTAAACACAC
nt 289 - nt 2469 of NM_009518.1
Notes:The Wnt10a probe used in this study was transcribed from the entire insert IMAGE clone 3976222, the sequence of which was verifed by Summerhurst and Murphy (it extends from nt 289 to 2469 relative to the Wnt10a cDNA RefSeq NM_009518.1).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE