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EMAGE:6164

Wnt16 wingless-related MMTV integration site 16 ( MGI:2136018)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:6164 EMAGE:6164 EMAGE:6164 EMAGE:6164 EMAGE:6164
3D reconstructed object showing signal. All sections along the X-axis, as movie. All sections along the Y-axis, as movie. All sections along the Z-axis, as movie. Photograph prior to 3D imaging.

View 3D opt image: EMAGE genex expression entry
Download 3D images in woolz format
Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
This is a traditionally produced wholemount in situ hybridisation stained using alkaline phosphatase + NBT/BCIP. It has been imaged using Optical Projection Tomography using visible light. In the OPT images, the signal is visible as the brightest regions.
Expression Pattern Description
Spatial Annotation:
EMAGE:6164Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
6164_voxel_strong_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6164_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: no stars
Text Annotation:
StructureLevelPatternNotes
embryo
detected detected
Annotation Validation: Submitter + EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:Wnt16 probeA
Entity Detected:Wnt16, wingless-related MMTV integration site 16 ( MGI:2136018)
Sequence:sense strand is shown

>Wnt16 probeA
ACGAGAGGTGGAACTGTATGGTCGCCACTACCACTTCCACCCAGCTCGCCACAGCCCCCCTCTTTGGCTA
TGAGCTGAGTAGCGGCACCAAGGAGACAGCATTCATTTATGCCATCATGGCAGCGGGCCTGGTGCACTCT
GTCACCAGGTCATGCAGTGCAGGCAACATGACCGAATGTTCCTGTGAAACCACCTTGCAGAATGGTGGCT
CACCAAGTGAAGGCTGGCACTGGGGAGGATGCTCGGATGATGTCCAGTACGGCATGTGGTTCAGCAGAAA
GTTTCTAGATCTTCCCATCAGAAACACCACAGGAAAAGAAAGCAGAGTCCTGCTAGCCATGAATCTACAC
AACAACGAAGCGGGGCGGCAGGCTGTCGCCAAGTTAATGTCTGTGGACTGCCGCTGCCACGGAGTTTCCG
GCTCCTGTGCTGTGAAAACCTGCTGGAAAACTATGTCTTCTTTTGAAAAGATTGGGCATTTTTTAAAGGA
TAAATATGAAAACAGCATCCAGATCTCAGACAAAACCAAGAGGAAAATGCGCAGGAGAGAAAAAGACCAG
AGGCAGACCCCCATTCTCAAGGATGACTTGCTGTACGTTCATAAGTCTCCCAACTACTGCGTGGAGAACA
AGAAACTGGGGATTCCTGGGACCCAGGGCAGAGAGTGCAACCGGACATCAGGAGGCGCAGATGGCTGTAA
CCTCCTCTGCTGTGGCCGAGGCTACAACACCCATGTAGTCAGGCACGTGGAGAGGTGTGAGTGTAAGTTT
ATCTGGTGCTGCTACGTCCGCTGCAGGAGGTGTGAAAGTATGACCGATGTCCACACGTGTAAGTAACCTC
TCCGTCCAGCCTAGCATGAGACGCCTCTGTAGTAACCAAGGTGTGGTGTTGGCATCTGGAGGGCGCCCCT
ACTGTGCACTGATGGGGAAGTCGCTGCCTGTAAGAGTGTTCCCAGACCCCTGGGCTAGTCTACGATTTCT
TTCTTTCTGGCAGGC
nt 537 - nt 1531 of NM_053116.3
Notes:The Wnt16 probe used in this study was generated by RT-PCR using the following primers: forward: 5'-ACGAGAGGTGGAACTGTATGG-3'; reverse: 5'-GCCTGCCAGAAAGAAAGAAATCG-3', and this fragment was then cloned using a TOPO TA cloning kit (Invitrogen). The probe was transcribed from the entire insert.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:opt
Procedures
Fixation:4% paraformaldehyde
Embedding:agarose
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kristen Summerhurst, Paula Murphy
Principal investigator:Paula Murphy, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Submitted by:Kristen Summerhurst, Trinity College Dublin, Zoology Department, Dublin, Ireland 2
Experiment type:non-screen
Links: Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE