EMAGE mouse gene expression spatial database

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EMAGE:6617

A430110N23Rik RIKEN cDNA A430110N23 gene ( MGI:3606211)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_016465. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_016465_01	euxassay_016465_02	euxassay_016465_03	euxassay_016465_04

euxassay_016465_05	euxassay_016465_06	euxassay_016465_07	euxassay_016465_08	euxassay_016465_09

euxassay_016465_10	euxassay_016465_11	euxassay_016465_12	euxassay_016465_13	euxassay_016465_14

euxassay_016465_15	euxassay_016465_16	euxassay_016465_17	euxassay_016465_18	euxassay_016465_19

euxassay_016465_20	euxassay_016465_21	euxassay_016465_22	euxassay_016465_23	euxassay_016465_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6617_wholemount_strong.wlz

6617_wholemount_moderate.wlz

6617_wholemount_weak.wlz

6617_wholemount_possible.wlz

6617_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6617_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hand mesenchyme

weak

regional

weak expression: see section 23 24

foot mesenchyme

weak

regional

weak expression: see section 23

medulla oblongata basal plate mantle layer

weak

regional

weak expression: see section 09 15 16

pons mantle layer

weak

regional

weak expression: see section 17

facial vii ganglion

moderate

single cell

moderate expression: see section 04 05

glossopharyngeal ix ganglion

moderate

single cell

moderate expression: see section 06 07 weak expression: see section 18

trigeminal v ganglion

moderate

single cell

moderate expression: see section 03 04 05 06 07 08 17 weak expression: see section 18 19 20

vagus x ganglion

moderate

single cell

moderate expression: see section 08 17

ventral grey horn

moderate

regional

moderate expression: see section 11 15 16 weak expression: see section 10 12 13 14

dorsal root ganglion

moderate

single cell

moderate expression: see section 08 09 10 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38164
Entity Detected:	A430110N23Rik, RIKEN cDNA A430110N23 gene ( MGI:3606211)
Sequence:	sense strand is shown >T38164 ACTCTGGGGAACAGTCTGTGATGACAGCTGGGACATCCGGGATGCCACAGTGGCCTGCTGGGAGCTGGGT TGCGGAAAGGTCCGGCCCCGAGTAGGCAAAACTCACTATGGCCCTGGCACTGGGCCCATCTGGTTGGATG ACATGGGCTGTAAAGGAAGTGAGATGTCACTGAGTGACTGCCCCTCGGGGGCATGGGGGAAGCACAACTG TGACCACGAAGAAGACGTGGTGCTCACCTGCACTGGCTACACGGGTGATGACGATTATCCTTCCTGGACC TGGGACCCTACTTCCGGAGAGGACCTGACCAAAGGGACCACAGTGGCTGCGCGGCCTGGACATACACTTT CCTGGGCTACCACTACAAACACTGAAGTCCCCTCTCCAGCAACACAGAACCTTCCAGACACGGATGACCA GGGAGGTTATGAGTCTTCCTGGACGTGGGATACACCTTCAGGAAGGGGTCTGTTCAAGGGGACCCCCACC ACAACCAAGCCTGGATCCACAGTTACCACTAGCACCAGCAAGAGTCCCGGCCACCCCTTTCCAGCTCCAA GGGCCCGTGCAGGTTCACCAAGGAAGCCAACACCTGAGCGCCGGCCGCTGCCCACCTCAGCCACCACCTC ATCTCCTGCTTCCTCCTCTTCACCAGAGCCTTCTGGCTCACGGCAGACCTCTGGCTCCTGGCCACAACTC ATCCCAGACTCAAAGCAGGAGGGGACCTCTAGCTCCCCCAAACCATCTCTACTCACCCCAGGACTTCCCT CCCCAGCAACCTTTGCCTTGTCCACTCCTAATACCAGTTTGCTTCCAACACGTTCCCCAGAGCTTTCAGG CAGCCCAACACCCACCTCCCCTGAAGGGCTAACCTCTGCCTCCTCTATGCTATCAGAAGTGAGCAGGCTT TCTCCTACCTCAGAGCTGACTCCAGGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 105167. Forward Primer - name:105167_F_cDNA_A430110N23, sequence:ACTCTGGGGAACAGTCTGTGAT; Reverse Primer - name:105167_N_SP6_cDNA_A430110N23, sequence:GTCCTGGAGTCAGCTCTGAGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6615 same embryo
	EMAGE:6616 same embryo
	EMAGE:6618 same embryo
	EurExpress:euxassay_016465 same experiment
	MGI:4822831 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source