EMAGE mouse gene expression spatial database

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EMAGE:6637

Arl4a ADP-ribosylation factor-like 4A ( MGI:99437)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007257. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007257_01	euxassay_007257_02	euxassay_007257_03	euxassay_007257_04

euxassay_007257_05	euxassay_007257_06	euxassay_007257_07	euxassay_007257_08	euxassay_007257_09

euxassay_007257_10	euxassay_007257_11	euxassay_007257_12	euxassay_007257_13	euxassay_007257_14

euxassay_007257_15	euxassay_007257_16	euxassay_007257_17	euxassay_007257_18	euxassay_007257_19

euxassay_007257_20	euxassay_007257_21	euxassay_007257_22	euxassay_007257_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6637_wholemount_strong.wlz

6637_wholemount_moderate.wlz

6637_wholemount_weak.wlz

6637_wholemount_possible.wlz

6637_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6637_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vertebral axis musculature

weak

spotted

weak expression: see section 02 03 04 05 06 07 08 09 10 15 16 18 19

diencephalon meninges

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17 18

thalamus mantle layer

weak

regional

weak expression: see section 08 09 10 11 12 13 14 15 16 17 18

telencephalon meninges

weak

regional

weak expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

midbrain meninges

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

spinal cord meninges

weak

regional

weak expression: see section 10 11 12 13 14 15

esophagus

weak

regional

weak expression: see section 12

mandible

moderate

regional

moderate expression: see section 04 06 07 08 09 16 17 18 weak expression: see section 05 19

maxilla

moderate

regional

moderate expression: see section 04 06 07 08 09 16 17 18 weak expression: see section 05 19

urethra of male

moderate

regional

moderate expression: see section 12 13

lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T2061
Entity Detected:	Arl4a, ADP-ribosylation factor-like 4A ( MGI:99437)
Sequence:	sense strand is shown >T2061 TGGCCTCGAGGCCAGATTCGGCACGAGGGTTCCTGTATTGGAAATGAGTTGGTTTATACAAAGTATTAAT TGGGTTAAACATTCTAATTTAAAATTACTACTAGCAAGCAAAGTTTTAAAATATCCTTTGATACTTAAAG AGTCATATATCCAAACTTATGCTAAAATAGATTATAAATGTTATGGTTTAGCCTAGATAAATTCATTAAT AATCATAAAATCCTTACAGGAAGCTAAAAACTGCAGTGTTAGGATATGTAGATGGAAGTCAATGACTACT AGTCTGTAGGTAACTGGATGATAGCTTAAGAATGAGAGTAAAGGGCACATAATTAGAATGTTTTGGTACG AGTTGGGATTATGGGTACAATGGGTGCTTGCCTATCAGGTACAGGGCTCTGGGTTTGAGTGCTAGCACTG CAAAGGAAAAATAAATCACTTACCAGAATGGCTTGTTTAGAAGAGTTTAATTGTTTATAGTGGCCATGAG AGAAATCTGGTATAGTGTATCAGTGTTCTATTTAATGTAATGGTTTTATCATATAAA
Notes:	The probe template was PCR amplified from IMAGE:820282 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:820282 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6636 same embryo
	EMAGE:6638 same embryo
	EMAGE:6639 same embryo
	EMAGE:6640 same embryo
	EMAGE:6635 same embryo
	EurExpress:euxassay_007257 same experiment
	MGI:4823238 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source