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EMAGE:6643

Rb1cc1 RB1-inducible coiled-coil 1 ( MGI:1341850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643
"Pseudo-wholemount" of euxassay_002229. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002229_01 euxassay_002229_02 euxassay_002229_03 euxassay_002229_04
EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643
euxassay_002229_05 euxassay_002229_06 euxassay_002229_07 euxassay_002229_08 euxassay_002229_09
EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643
euxassay_002229_10 euxassay_002229_11 euxassay_002229_12 euxassay_002229_13 euxassay_002229_14
EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643 EMAGE:6643
euxassay_002229_15 euxassay_002229_16 euxassay_002229_17 euxassay_002229_18 euxassay_002229_19

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6643Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6643_wholemount_strong.wlz
6643_wholemount_moderate.wlz
6643_wholemount_weak.wlz
6643_wholemount_possible.wlz
6643_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6643_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 17
trigeminal v ganglion
strong strong
regionalstrong expression: see section 03 04 05 moderate expression: see section 06 07 08 16 17 18
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 08
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 07 08 09 14 15 16 17
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1949
Entity Detected:Rb1cc1, RB1-inducible coiled-coil 1 ( MGI:1341850)
Sequence:sense strand is shown

>T1949
GGACAATGTTACATCTTTGCATCGCCAAGCATATGAAATAGAAAAAAAACTGAAAGAACNAATAGTTGAA
TTGCAGACTAGATTGAACTCAGAATTGAGTGCTCTTGAAAAACAGAAAGATGAAAAAATAACCCAACAAG
AAGAGAAGTATGAAGCACTTATCCAGAACCTTGAGAAAGACAAGGAGAGACTGGTCAAGAACCACGAGCA
AGACAAAGAACACTTAATTCAGGAGCTTAATTTTGAAAAAAACAAAGCTGTTCANACTGCACTAGATGAA
TTTAAGGTGGAGAGAGAACTTGTTGAGAAAGAGTTATTAGAAAAAGTTAAACATCTTGAGAATCAAATAG
CCAAAACTCCTGCCTTTGAGTCAGCCAGAGAAGATTCTTCAAGCTTAGTTGCGGAACTTCAAGAGAAACT
TCAAGAAGAAAAAGCTAAGTTTCTGGAACAACTTGAAGAACAAGAGAAAAGAAAGAATGAGGAAATGCAA
AATGTCAGAACCTCTTTGATTGCTGAGCAGCAG
Notes:The probe template was PCR amplified from IMAGE:639545 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:639545 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:BL6/SV129
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6642 same embryo
 EMAGE:6645 same embryo
 EMAGE:6641 same embryo
 EMAGE:6644 same embryo
 EurExpress:euxassay_002229 same experiment
 MGI:4827644 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS