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EMAGE:6734

Map2k1 mitogen-activated protein kinase kinase 1 ( MGI:1346866)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734
"Pseudo-wholemount" of euxassay_002349. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002349_01 euxassay_002349_02 euxassay_002349_03 euxassay_002349_04
EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734
euxassay_002349_05 euxassay_002349_06 euxassay_002349_07 euxassay_002349_08 euxassay_002349_09
EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734
euxassay_002349_10 euxassay_002349_11 euxassay_002349_12 euxassay_002349_13 euxassay_002349_14
EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734 EMAGE:6734
euxassay_002349_15 euxassay_002349_16 euxassay_002349_17 euxassay_002349_18 euxassay_002349_19
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euxassay_002349_20 euxassay_002349_21 euxassay_002349_22 euxassay_002349_23 euxassay_002349_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6734Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6734_wholemount_strong.wlz
6734_wholemount_moderate.wlz
6734_wholemount_weak.wlz
6734_wholemount_possible.wlz
6734_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6734_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T1925
Entity Detected:Map2k1, mitogen-activated protein kinase kinase 1 ( MGI:1346866)
Sequence:sense strand is shown

>T1925
TGGCCTCGAGGCCAGATTCGGCACGAGGTATGGTTACTCCCCTAAGTGGATTGGCTTTGTGCTTGGGGCT
ATTTGTCTGTTCATCAAACACATGCCAGGCTGAACTACAGTGAAACCCTAGTGACCTGGGTGGTCGTTCT
TACTGATGTTTGCACTGCTGTTCATCGTGACTCACTAGCTGGCTGCCTGTATTGTCAGGATTCTCGGACC
TTGGTACTTCACTCTTGCTGGTGACCTCTCAGTCTGAGAGGGAGCCTTGTGAGACCCTTCACAGGCAGTG
CATGCATGGAAAGCATGCTTTGCTGCTACTGAAATGAGCATCAGAACGTGTACGTCATGGTATTTTTATT
TTTTGCTTTTGGTATAGAACTCAGCAATTCCCATCAAAAAAACCTAAGCAGAGCCCATCACTGCCATGAT
AGCTGGGCTTCAGTCTGTCTACTGTGGTGATTTTTAGACTTCTGGTTGTATTTCTATATTTATTTTTAAA
TATACAGTGTGGGATACTTAGTGGTGTGTGTCTCTAAGTTTGGATTAGTGTTTCTAAATTGGTGGTTATT
TT
Notes:The probe template was PCR amplified from IMAGE:634946 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:634946 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6733 same embryo
 EMAGE:6736 same embryo
 EMAGE:6732 same embryo
 EMAGE:6731 same embryo
 EMAGE:6735 same embryo
 EurExpress:euxassay_002349 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS