EMAGE mouse gene expression spatial database

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EMAGE:6844

Lsm11 U7 snRNP-specific Sm-like protein LSM11 ( MGI:1919540)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002656. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002656_01	euxassay_002656_02	euxassay_002656_03	euxassay_002656_04

euxassay_002656_05	euxassay_002656_06	euxassay_002656_07	euxassay_002656_08	euxassay_002656_09

euxassay_002656_10	euxassay_002656_11	euxassay_002656_12	euxassay_002656_13	euxassay_002656_14

euxassay_002656_15	euxassay_002656_16	euxassay_002656_17	euxassay_002656_18	euxassay_002656_19

euxassay_002656_20	euxassay_002656_21	euxassay_002656_22	euxassay_002656_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6844_wholemount_strong.wlz

6844_wholemount_moderate.wlz

6844_wholemount_weak.wlz

6844_wholemount_possible.wlz

6844_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6844_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 01 02 18 weak expression: see section 03 04 05 06 07 08 19 20 21 22 23

femur

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08

viscerocranium

moderate

regional

Expression in the turbinate bone.

nucleus pulposus

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17

pectoral girdle and thoracic body wall skeleton

moderate

regional

moderate expression: see section 01 02 03 04 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5044
Entity Detected:	Lsm11, U7 snRNP-specific Sm-like protein LSM11 ( MGI:1919540)
Sequence:	sense strand is shown >T5044 CAAACATGGAGGAGCGCGAGTGGGGGGCGAGGTCGGCTCGCGCCGGCAGCCCAGCCAGCCCGCCCAGCCC GCGGCTGGACGTCAGCTCCTACAGCTTCGACCCGCTGCTGGCCTTGTACGCACCTCGCCTGCCGCCCATT CCCTACCCCAACGCGCCCTGCTTCAACAACGTGGCGGAGTACGAGAGCTTCCTGAAGGGCGGGCGCACCG GGCGCGGTCGGGCTCGGGGCACGGGGGAGCCGGCCTCGGCGGGAACCTCCACCGGAACCTCCACGGGAGC CGGGAGCTCGTCCAGGGCCCGTCGCCGCGCCGCGCCCACCCCGGATCCCGAGCGCATCCAGCGCCTGCGC CGGCTCATGGTGGTCAAGGAGGACACGGACGGCACGGCCGGAGCGCGACGCCAGGGCCCGGGGCGCAGCA AGAAGGCACCCCGCAATGTGCTTACGCGGATGCCTTTGCATGAAGGTAGCCCTCTGGGTGAACTGCACCG CTGCATCCGAGAGGGAGTCAAGGTGAACGTTCACATCCGCACCTTCAAGGGACTCCGCGGTGTCTGCACT GGCTTCCTCGTCGCCTTCGACAAGTTCTGGAATATGGCCCTCACTGACGTGGATGAGACCTACCGTAAAC CTGTCTTAGGAAAAGCATACGAACGGGATTCTTCGCTGACTCTCACTAGGCTATTTGATCGGCTGAAACT TCAAGATTCCTCCAAAAAGGAAGCGGATTCCAAATCTGCAGTTGAGGACTCCACCCTGTCTCGATACTCC CAAACATCTACTTGGAAGGTGGCTTCGGTATGGGGACGAGGGGACACTGACCGGAGCTCACACAGGCGCT CCCGCTCCGTCCCTTCTTCCCTGCAGGCATCTGCAAGGGAGGAGTCCAGGTCTGAGCTGTCAGGGAGGAC TACACGGACAGAAGGGTCCAGTGTGGGAGGTACCTTCTCCAGGGCCACCACCCTTTCACGGGGCCAGTCC CGTAAGAAAAAGCGAAAGCCCAAGGTGGATTACCAGCAGGTATTCACTCGGCACATAAATCAGATTTTCA TTCGAGGCGAGAATGTCCTGCTGGTCCATCTGGCCCAGTGAGCAGCTCAGCCCACGTGACCTTTGGGTTG TAGAAATAAAGTGCATGAACTGTTCTCTGCTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5294386 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5294386 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6845 same embryo
	EMAGE:6846 same embryo
	EMAGE:6848 same embryo
	EMAGE:6843 same embryo
	EMAGE:6842 same embryo
	EMAGE:6847 same embryo
	EurExpress:euxassay_002656 same experiment
	MGI:4826012 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source