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EMAGE:6860

4632415L05Rik RIKEN cDNA 4632415L05 gene ( MGI:1918058)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860
"Pseudo-wholemount" of euxassay_002647. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002647_01 euxassay_002647_02 euxassay_002647_03 euxassay_002647_04
EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860
euxassay_002647_05 euxassay_002647_06 euxassay_002647_07 euxassay_002647_08 euxassay_002647_09
EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860
euxassay_002647_10 euxassay_002647_11 euxassay_002647_12 euxassay_002647_13 euxassay_002647_14
EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860 EMAGE:6860
euxassay_002647_15 euxassay_002647_16 euxassay_002647_17 euxassay_002647_18 euxassay_002647_19
EMAGE:6860 EMAGE:6860 EMAGE:6860
euxassay_002647_20 euxassay_002647_21 euxassay_002647_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6860Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6860_wholemount_strong.wlz
6860_wholemount_moderate.wlz
6860_wholemount_weak.wlz
6860_wholemount_possible.wlz
6860_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6860_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T5024
Entity Detected:4632415L05Rik, RIKEN cDNA 4632415L05 gene ( MGI:1918058)
Sequence:sense strand is shown

>T5024
GTCCAGAGGTCCGTCTGTGGTTTGGGGGTATTTGGGTGCCCCTGGAATGAAGTACGTTCTGAAGTATGCT
GCACCGAAGTGAGGGGACCGGGAGTCATGGAGGGCCAGAGCGCAGAGATGCTGCAGGCCAGGTGGAGGCG
GAGAAGTTGCAGCGCCTCCCAGTGCACAAAGAGCTGGAGCAAGAATTCGACCTTGGCACCCTACCGGCTC
CGGACCGCACTCCCGGACAGGCCGAGCTGCCGGCCTTGGCGCGGGACAGCACGCAGCTGCTCATCACCGT
GCTGCTGCCGACCGTGCGCTGGGGGAGGCGGTGGTAGCGCGCTTGCCGGTGCCCTCCACTCGCTTGCCCC
GCGGGAAGCTGCTATCCGTGCCGCGGCTGCTCACCCGCTGGCAGCAGTTCGCGCGTCATCAGAGCATCCA
CCCAAGAAGACCACTTTCTTGTGGGACGAGGTGAGTGGCCAGTGACGGTGCTGATTGGACTGCAAGCTCG
CCCGGAACCACAGTATGGAATGGCTGATAAATGTGCCTGGGGGCACCCGACCCCATGGAAGCAGATTTAG
GCCAAGAAGGAACGCGTGGCCAAGAACGAGCTAAAACGACCGCGGATCCGAGTGCACAAGATGTAGATGC
CCAACTCAGTCGGCCTGCACCCAACGAGACACCTGAGTAAGGAGGAGCTGGTCTGCGCCCAGCAAGTGGC
CAGTGACTCCACCGCTGCAGTGGGACGCTTCCAGGAGCGCCTCTCTAAGGAGAAGACGCCCCTAGAGCCC
CGGAAGAAGAGGAAGTTTTAGTCCCTCTTTGAGGACTTTGTGACCGAGAAAATGACCAGTTGGAGCTACT
GAAAGTCAAGAACAGCAAGAAGCCTCATCAGGATGTGAGGGGGCGCATCAACAAGCAGATGAGAGAAGAG
GAAAATGAGCCAGAAAGGCGAAGAGAAAGGGGATCCAGCAAAGATCTTTGAGTAAGAGCAGGGGCTGGAG
AGATGGCTTAGCAGTTAAGAGCACTGACTTCCCTTCCAGAGGTCCTGAGTTCAATTCCCAGCAACCACAT
GGTGGCTAAATAATAAATAATAAATCTAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5252327 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5252327 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6861 same embryo
 EMAGE:6858 same embryo
 EMAGE:6863 same embryo
 EMAGE:6862 same embryo
 EMAGE:6859 same embryo
 EurExpress:euxassay_002647 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS