EMAGE mouse gene expression spatial database

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EMAGE:6861

Kcnk13 potassium channel, subfamily K, member 13 ( MGI:2384976)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002451. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002451_01	euxassay_002451_02	euxassay_002451_03	euxassay_002451_04

euxassay_002451_05	euxassay_002451_06	euxassay_002451_07	euxassay_002451_08	euxassay_002451_09

euxassay_002451_10	euxassay_002451_11	euxassay_002451_12	euxassay_002451_13	euxassay_002451_14

euxassay_002451_15	euxassay_002451_16	euxassay_002451_17	euxassay_002451_18	euxassay_002451_19

euxassay_002451_20	euxassay_002451_21	euxassay_002451_22	euxassay_002451_23	euxassay_002451_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6861_wholemount_strong.wlz

6861_wholemount_moderate.wlz

6861_wholemount_weak.wlz

6861_wholemount_possible.wlz

6861_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6861_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5021
Entity Detected:	Kcnk13, potassium channel, subfamily K, member 13 ( MGI:2384976)
Sequence:	sense strand is shown >T5021 CGGACGCGTGGGTCCCGCGGGCAGCAACCCTCGCGGAGCTGTCCTCCAGTGCCATGGCTGGCCGCGGTTG CGGCTGCAGCCCCGGCCACCTGAACGAGGACAACGCGCGCTTCCTGCTGCTCGCTGGGCTCATCCTGCTC TACCTGCTGGGCGGCGCCGCAGTCTTCTCCGCGCTGGAGCTAGCGCAGGAGTTGCAGGCCAAGCAGCGCT GGGAAGAGCGCCTGGCCAACTTCAGCCGGGGCCACAACCTGAGCCGTGAAGAGCTGCGAGGTTTCCTCCG CCACTACGAGGAAGCCACCAGGGCGGGCATCCGCATGGACAGCGTGCGCCCTCGCTGGGACTTCACGGGC GCCTTCTACTTCGTGGGTACAGTGGTTTCTACCATAGGGTTTGGGATGACAACACCAGCCACAACGGGAG GGAAGATTTTTCTGATCTTTTATGGTCTCATTGGATGTGCAAGTACCATCCTCTTCTTCAACCTTTTCCT GGAGCGGCTGATCACTGTCATCGCCTGTGTCATGAGATCCTGTCACCAGCAGCAGCTGCGCAGACGTGGG GCGGTGACCCAGGACAACATGAAGGCTCCTGAAAAGGGGGAGGCAGACAGCCTGACTGGCTGGAAGCCCT CTGTGTACTACGTCATGCTGATCCTATGCTTGGCATCAGTGGCCATCTCCTGCGGAGCCTCTGCTCTGTA CACCACCATGGAGGGCTGGAGCTACTTTGACTCGGTCTACTTCTGTTTTGTGGCTTTCAGCACCATTGGC TTCGGGGACCTGGTGAGCAGCCAGAATGCTCAGTATGAGAGCCAAGGACTCTACCGCTTCTTCAATTTCT TCCTCATCCTCATGGGTGTCTGCTGCATCTACTCTTTGTTTAACGTCATCTCCATCCTGATCAAACAGAC TGTGAACTGGATCCTGAGGAAACTGGATAGCGGGTGCTTCCCACCATGCCAAAGAGGACTCCTGCGGTCC AGGAGGAATGTGGTGATGCCGGGTAACATCCGGAACAGGTGCAACATCTCCATAGAGACAGACGGGGTGA TGGAAAGTGACACTGATGGACGACGTCTCTCGGGGGAGATGATCTCCATGAAGGACACCAACAAGGTCTC CCTGGCCATCCTGCAGAAGCAGTTGTCCGAGATGGCCAATGGGGGACCCCACCAGAACAGTGCATCCTCC CGGGATGATGAGTTCTCAGGGGGAGTGGGAGCCTTTGCAGTAATGAATAACAGGTTGGCAGAGACCAGTG GAGATAGGTAGGAACCAGAAGTAGCTGCTATATGAAGAAAACCAGGGCATGTGGGGAATAAGACACTGTT ACACCACTGGTAAGCTCAGCTGTGCCTCTGGCTGTTGCAATTAATATCTCCAGTCCAGGTTTTGAAATCT GACCTTGGCCTCAGGCAAGAGTAGCCTCTCACAGTTGAGGGCTGGAGCCTCTTTCCCTGGCTCTTACTTT ACTTTTGAATTCCAAGTTTGACATTTGGAACTGCTAAGGGCCTTGCCTTCTTCCAGAGTTGTAATGGTCT GAGGGAGACTGGTCCAGTTTTGGTTTCTCCACCATGATCCATTCTTAGGAAGGGAGAGCCCCCAAAAAGC CTGCTTCTGTCCTGGAACGTGAGTGGCTGAACTTCACCCAGCCCCTCAAGAAAAGGCAACAAACATTCCA GAGTGTCTCTGGGCCTTCTGTTTGCTGGACTTCCTTCCAAACATATCTATTGTTGGTTCCATTGATGGGG ACACTATCTCATCTTCTCTTATACCTGCTTCTCTGTTTGGGAGTCTTGGTTTCTTTTTGTTTTGTTTCCT GTTTGACTAATATATCTTTTGGATCTTATGTGATAAGAAAACTAAATCCACCTTCAAACCTTTAAGCTTG GCAGTGAGTAGAGGAAGAGGGAAACTCCATCTTGTAACTAAAGCCCACAGCTTACTATGTCCTCTCTGAA TGGTTACACGGATCGTGTCATTTTACCAGGGCTTGGAGCCTGGTGCAGAGGCAACTGACTATTTATTAGA GTTGAACATTTAATAAATTCTCATTTTGTTAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5251817 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5251817 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6858 same embryo
	EMAGE:6863 same embryo
	EMAGE:6862 same embryo
	EMAGE:6860 same embryo
	EMAGE:6859 same embryo
	EurExpress:euxassay_002451 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source