EMAGE mouse gene expression spatial database

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EMAGE:6867

Ripk2 receptor (TNFRSF)-interacting serine-threonine kinase 2 ( MGI:1891456)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002417. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002417_01	euxassay_002417_02	euxassay_002417_03	euxassay_002417_04

euxassay_002417_05	euxassay_002417_06	euxassay_002417_07	euxassay_002417_08	euxassay_002417_09

euxassay_002417_10	euxassay_002417_11	euxassay_002417_12	euxassay_002417_13	euxassay_002417_14

euxassay_002417_15	euxassay_002417_16	euxassay_002417_17	euxassay_002417_18	euxassay_002417_19

euxassay_002417_20	euxassay_002417_21	euxassay_002417_22	euxassay_002417_23	euxassay_002417_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6867_wholemount_strong.wlz

6867_wholemount_moderate.wlz

6867_wholemount_weak.wlz

6867_wholemount_possible.wlz

6867_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6867_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5048
Entity Detected:	Ripk2, receptor (TNFRSF)-interacting serine-threonine kinase 2 ( MGI:1891456)
Sequence:	sense strand is shown >T5048 CCCACGCGTCCGCCCACGCGTCCGCACAGCTGACCGGGCCGGAACTGCAGCGGCCACGCGGGAATGGGCC GCCCCGGGACCTAGCGCCGCGGCCAGGGTCGGGCGGAGCCGCCGCGCAGCCGGAGCCATGAACGGGGACG CCATCTGCAGCGCGCTACCCCCCATCCCGTACCACAAGCTCGCCGACCTGCACTACCTCAGCCGGGGCGC CTCTGGCACGGTCTCGTCGGCACGACACGCAGACTGGCGCGTGCGGGTGGCAGTGAAGCACCTACACATC CACACGCCGCTGCTCGACAGTGAAAGAAATGATATCTTACGAGAAGCCGAAATATTACATAAAGCAAGAT TTAGTTACATTCTTCCAATTTTGGGAATTTGCAATGAGCCTGAATTTTTGGGCATAGTTACTGAATACAT GCCAAATGGATCATTAAATGAGCTTCTACACAGAAAAACTGAATATCCTGATATTGCATGGCCATTGAGA TTCCGCATCCTGCATGAAATTGCTCTTGGTGTAAATTATCTGCACAATATGAACCCCCCTCTGCTTCATC ATGACCTAAAGACTCAGAATATCTTGTTGGATAATGAATTTCATGTTAAGATTGCAGATTTTGGTTTATC AAAATGGCGCATGATGTCACTCTCTCAATCACGAAGTTACAAATCTGCACCCGAAGGAGGAACAATCATC TATATGCCACCTGAGAACTATGAGCCAGGACAGAAATCAAGGGCCAGTGTGAAGCATGATATATACAGCT ATGCAGTTATCATGTGGGAAGTGTTATCCAGAAAACAGCCTTTTGAAGAGGTCACCAATCCTTTGCAGAT CATGTACAGTGTGTCCCAGGGACATCGACCTGACACCAGTGAGGAGAATTTGCCATTTGATATACCTCAT CGAGGTCTCATGATCTCTCTAATACAGAGTGGATGGGCGCAAAACCCAGATGAAAGACCATCCTTTTTGA AATGCTTAATAGAACTTGAACCAGTTCTGAGAACATTTGAAGACATAACTTTCCTTGAGGCTGTTATTCA GCTAAAGAAAGCAAAGATACAGAGTTCCTCAAGTACTATTCACTTATGTGATAAGAAAATGGATTTATCG CTAAACATACCTGCAAATCATCCCCCACAGGAGGAATCGTGTGGATCCTCTCTGCTCTCTAGAAACACTG GTTCTCCTGGACCCTCCAGATCCCTATCAGCTCCTCAAGACAAGGGATTTTTATCTGGAGCACCTCAAGA CTGCTCTTCGTTGAAGGCACACCACTGTCCTGGAAACCACAGCTGGGATGGTATCGTTTCTGTACCACCA GGGGCAGCCTTCTGTGATCGCAGGGCCTCCTCGTGTTCCTTGGCTGTAATAAGTCCATTCTTGGTTGAGA AAGGTTCAGAGCGCCCACCAATTGGTATAGCCCAACAGTGGATCCAGAGTAAGAGGGAAGCCATTGTGAG CCAGATGACTGAAGCCTGCCTTAACCAATCGCTGGACGCCCTTCTGTCTCGTGACCTGATCATGAAGGAG GACTATGAACTCATTAGCACCAAACCTACAAGGACCTCAAAAGTCAGACAGCTGCTAGATACGTCTGACA TCCAAGGAGAAGAGTTTGCCAAAGTTGTAGTGCAGAAGTTGAAAGATAACAAGCAGCTGGGGCTGCAGCC TTACCCAGAAGTACCTGTGCTTTCCAAAGCACCGCCTTCAAATTTCCCTCAGAATAAAAGCTTGTAAGGG GGCCTTCTTTGGGAGGAAGTCAGTTTACAAATGGTTATTTATATTTCTGTTACCTGTATAATATTTGTAT AGAAAACATGAGAGTATTAGACGCTTCACTGAAGGTTGATGGAAGAGTCTCTTCCCATGACGTTGCAGTA TTTTTTTAAATTAATATAAGTAAAAAGTTTGCTTTAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5316066 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5316066 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6869 same embryo
	EMAGE:6868 same embryo
	EMAGE:6871 same embryo
	EMAGE:6870 same embryo
	EMAGE:6866 same embryo
	EurExpress:euxassay_002417 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source