EMAGE mouse gene expression spatial database

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EMAGE:6868

Arhgap24 Rho GTPase activating protein 24 ( MGI:1922647)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002529. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002529_01	euxassay_002529_02	euxassay_002529_03	euxassay_002529_04

euxassay_002529_05	euxassay_002529_06	euxassay_002529_07	euxassay_002529_08	euxassay_002529_09

euxassay_002529_10	euxassay_002529_11	euxassay_002529_12	euxassay_002529_13	euxassay_002529_14

euxassay_002529_15	euxassay_002529_16	euxassay_002529_17	euxassay_002529_18	euxassay_002529_19

euxassay_002529_20	euxassay_002529_21	euxassay_002529_22	euxassay_002529_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6868_wholemount_strong.wlz

6868_wholemount_moderate.wlz

6868_wholemount_weak.wlz

6868_wholemount_possible.wlz

6868_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6868_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5075
Entity Detected:	Arhgap24, Rho GTPase activating protein 24 ( MGI:1922647)
Sequence:	sense strand is shown >T5075 CCACGCGTCCGGAGAGAAAGCCGGTGGGACTCGGAAGGGAATCACGGGGCCGCGGGGCCGCCCAGGCTGC AGAGGGGACAGAACTAGAGCGAGACAGCGACTGCTGGGGGAGTCCCCCCCAACTTTCCATGCAGCTGTGG CTCTGGGGCTGCCCGGGCTTCCTCTGAGAGCAGCACGCAGGCCTGGCGAATGGGGTTCTGCGCTCGCCCT GGGAAGTAAAGGGCACCCTGCCGACTGGAACTCATCTTTAGGCCAGTCCTAGGAGCCTGGACATCGGAGC AGCCGCCAGCCATCCCTTGTAGGTCCGGCATCCCTGGCAGGGGGACTATTTTTCTGCATGGAAATAAAGT CATTGAGCATCCGTGCAATGAAGAAAACCCAGGAAAGTTCCTTTTCGAAGTAGTTCCAGGAGGTGAACGA GATCGGATGACAGCCAATCATGAGAGCTATCTCCTCATGGCGAGCACCCAGAATGACATGGAAGACTGGG TGAAGTCCATCCGCCGAGTCATATGGGGACCTTTTGGAGGAGGTATTTTTGGACAGAAACTGGAAGACAC AGTCCGATATGAAAAGAGGTACGGGAACCGGCTGGCACCCATGCTGGTGGAGCAGTGTGTGGACTTCATC CGACAGCGGGGGCTGAAGGAAGAAGGCCTCTTCCGGCTGCCGGGCCAGGCGAACCTGGTCAAGGAGCTCC AGGATGCGTTCGACTGTGGAGAGAAGCCCTCCTTCGACAGCAACACCGACGTCCACACTGTGGCATCCCT GCTGAAATTGTATCTCCGAGAACTTCCAGAGCCCGTCGTTCCTTATGCAAAGTATGAAGACTTTTTGTCC TGCGCCACGCTGCTCAGTAAGGAGGAAGAAGCAGGTGTTAAGGAGCTAATGAAACAAGTGAAGAGTTTGC CTGTGGTTAACTACAACCTTCTGAAGTACATTTGCAGGTTCCTGGATGAAGTGCAGTCCTATTCAGGAGT TAACAAGATGAGCGCACAGAACTTGGCAACTGTCTTCGGTCCTAACATCCTGCGCCCAAAGGTGGAAGAT CCCTTGACCATCATGGAGGGCACTGTGGTGGTCCAGCAGTTAATGTCAGTGATGATTAGTAAGCATGATC GACTCTTCCCCAAAGACACAGAACCACAAAGCAAGCCCCAAGACGGCCCGAACAGCAATAATAACGATGG TCATAAGAAAGCCACCATGGGTCAGTTGCAGAACAAAGAAAACAATAATACCAAGGAGAGCCCCGTCAGA AGGTGCTCTTGGGACAAGCCTGAATCCCCCCAGAGAAGCAGCGTGGACAATGGATCCCCCACAGCCCTAT CAGGCAGCAAAACCAACAGCCCAAGGAACAGTATCCACAAGCTGGATATTTCCAGGAGCCCCCCTCTCAT GGTCAAAAAGAACCCAGCCTTCAACAAGGGCAGTGGGATTGTCACCAACGGGTCATTCAGCAGCAGCAAT GCAGAAGGTGTCGAGAAGCCTCAAACTACTCCTAATGGGAGTTTACAAGCCAGAAGGACCTCTTCACTGA AGAGTTCTGGTACCAAAATGGGCACTCACAGTGTACAGAATGGCACTGTCCGCATGGGTATTCTGAACAC TGACACACTAGGGAATTCCTTGAACGGCCGGAGCATGAGTTGGCTGCCCAATGGCTATGTGACCCTGAGA GATAACAAACAGAAGGAGCCAGCAGGAGAGTCAGGCCAGCACAACAGACTCTCCACCTACGACAATGTCC ACCAGCAGTTCTCCTCGATGAGCCTCGACGACAAGCACAGCGTGGACAGTGCCACGTGGTCCACCTCCTC CTGCGAAATCTCCCTCCCTGAGAACTCCAATTCCTGCCGCTCCTCTACCACCACGTGCCCAGAGCAAGAC TTCTACGTTGGGAACTTTGAGGACCCTGTTTTAGACGGCCCACCTCAGGATGATCTTTCCCATCCCGGGG ACTATGAGAACAAAAGTGACCGCAGGAGCGTAGGGGGCCGAAGCAGCCGCGCCACGAGCAGCAGCGATAA CAGCGAGACATTCGTGGGCAACACCAGCAGCAACCACAGTGCACTTCACAGTTTAGTTTCCAGCCTGAAG CAGGAGATGACCAAACAGAAGATCGAGTATGAGTCCAGGATAAAAAGCTTAGAACAGCGGAATTTGACTT TGGAAACGGAAATGCTGAGCCTCCATGACGAACTGGATCAAGAGAGGAAAAAGTTCACCATGATAGAAAT CAAGATGAGAAACGCTGAGCGCGCAAAGGAGGATGCGGAGAAAAGGAACGACATGCTCCAGAAGGAGATG GAGCAGTTCTTCTCCACATTTGGAGACCTGACAGTGGAACCCAGGAGAAGCGAGAGAGGAAACACAATCT GGATCCAGTGAGCCTTTCTCTGTCCTGGACCGCTCGCTCCGCGGAGGACTCTGGGGACTCCGGGGCAAAC AGTGTTGGGGAACAGGTGTGGGATCAGGTGGTTGGTCTGTGTAGAGCTTCATCTGGTGCAGGAATCTCAT TTCCAGACATCACTATCCATATCTGCAGTGTGTACCAAAGTTATATCATGCTCCATAATGCTACTGTCAA GTGTTACAACTGGATATGTGTATAGAGAGTAGTTTCTAAGATGTCAATAAGAGTAAGAAGCATATATCTC AATGATTATTTTATTGCAAGTCCTGTATTTAAATGTCGAATCAATACTTTGTTGCAATTTAGCTTGCATT CAAGCTTCACCCCGTGCACTTAACATAAGCTATTTTTGGCATCGTGTTATCATCTGCTTATTTTATAGAT CAATATTTTTATTTCCCCTTTTGTCGAGGAAATGGAGATAAGAAGAGATATAAATATATAAATATATATA AGATGAGTTATTAAAGTCAAAAGAATAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5321985 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5321985 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6869 same embryo
	EMAGE:6867 same embryo
	EMAGE:6871 same embryo
	EMAGE:6870 same embryo
	EMAGE:6866 same embryo
	EurExpress:euxassay_002529 same experiment
	MGI:4823219 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source