EMAGE mouse gene expression spatial database

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EMAGE:6871

B4galnt1 beta-1,4-N-acetyl-galactosaminyl transferase 1 ( MGI:1342057)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002539. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002539_01	euxassay_002539_02	euxassay_002539_03	euxassay_002539_04

euxassay_002539_05	euxassay_002539_06	euxassay_002539_07	euxassay_002539_08	euxassay_002539_09

euxassay_002539_10	euxassay_002539_11	euxassay_002539_12	euxassay_002539_13	euxassay_002539_14

euxassay_002539_15	euxassay_002539_16	euxassay_002539_17	euxassay_002539_18	euxassay_002539_19

euxassay_002539_20	euxassay_002539_21	euxassay_002539_22	euxassay_002539_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6871_wholemount_strong.wlz

6871_wholemount_moderate.wlz

6871_wholemount_weak.wlz

6871_wholemount_possible.wlz

6871_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6871_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

moderate

homogeneous

moderate expression: see section 11 12 14 15 weak expression: see section 13

foregut-midgut junction

strong

regional

strong expression: see section 07 08 09 12 13 14 15

brain

strong

homogeneous

strong expression: see section 01

diencephalon

strong

homogeneous

strong expression: see section 08 09 10 11 12 13 14 16 17

telencephalon mantle layer

strong

homogeneous

strong expression: see section 03 04 05 06 07 08 19 20 21 22 23

telencephalon marginal layer

strong

homogeneous

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13 15 16 17 18 19 20 21 22 23

olfactory cortex marginal layer

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16 17 18

hindbrain

strong

homogeneous

strong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain

strong

homogeneous

strong expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

facial vii ganglion

weak

regional

weak expression: see section 04 19

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 07

trigeminal v ganglion

weak

regional

weak expression: see section 04 05 06 07 08 09 17 18 19

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 08 17 18

trigeminal v nerve

weak

regional

weak expression: see section 20 21

spinal cord

strong

homogeneous

strong expression: see section 09 10 11 12 13 14 15 not examined expression: see section 16

dorsal root ganglion

weak

regional

weak expression: see section 08 09 16 17

not examined

regional

not examined expression: see section 02 03 04 22 23

neural retina

moderate

regional

moderate expression: see section 02 03 04 22 23

stomach

strong

regional

strong expression: see section 05 06

hindgut

strong

regional

strong expression: see section 12 13 14 15

midgut

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17

lower jaw incisor

strong

regional

strong expression: see section 12 13 15 16

lower jaw molar

strong

regional

strong expression: see section 07 08 18 19

upper jaw incisor

strong

regional

strong expression: see section 12 13 16 17

upper jaw molar

strong

regional

strong expression: see section 07 08 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5047
Entity Detected:	B4galnt1, beta-1,4-N-acetyl-galactosaminyl transferase 1 ( MGI:1342057)
Sequence:	sense strand is shown >T5047 CCTGGGAGCGCGACGCGGTGCCAGGGCTTCCCCGCTTTGTCGCTGGGGCTGGGGCGCGGCGGATTTACGA TCCAGTTCACCCCGGCAGGAAACGTTCAGAACCACCCCCGCGCTGCGGTCTACAAAGCCCCGGAGTCCGC AGGCGGGAGGAGTCGGATCCCCGACACTGTCGGGCTCCCTCCCGCCCAGCTCCCGCATCAGGCCGTCGCC GGCCTCATGAGGACCCCCCGGCCGGGGCGGAGAACCGGGCACTGCCCGGACCGCGATCCTGCCGCGGCCT TAGAACGTTAGACAGGATGCGGCTAGACCGCCGGGCCCTCTATGCGCTAGTCTTGCTGCTCGCCTGCGCC TCGCTGGGTCTCCTGTACTCCAGCACCCGAAACGCGCCAAGTCTCCCGAACCCTCTGGCATTGTGGTCGC CCCCACAAGGTCCCCCGAGACTCGATCTGCTAGACCTTGCCCCTGAGCCTCGCTACGCACACATCCCGGT CAGGATCAAGGAGCAAGTGGTGGGGCTGCTGGCTCAGAACAACTGCAGTTGTGAATCCAAGGGAGGAAGC CTTCCCTTGCCCTTTCTGAGACAGGTTCGGGCGGTTGACCTCACTAAAGCCTTTGACGCTGAGGAGCTGA GGGCTGTTTCTGTCGCCAGGGAGCAGGAATACCAGGCCTTCCTTGCAAGGAGCCGGTCCCTGGCTGACCA GCTGCTGATAGCTCCCGCCAACTCCCCCTTACAGTACCCCCTGCAGGGTGTGGAGGTTCAGCCCCTCAGG AGCATCCTGGTACCAGGGCTAAGTCTGCAGGAAGCTTCTGTCCAGGAGATATACCAGGTGAACCTGAGTG CTTCTCTAGGCACCTGGGATGTGGCAGGGGAAGTAACAGGAGTGACTCTCACGGGAGAGGGGCAACCGGA TCTCACCCTTGCCAGCCCAGTTCTGGATAAACTCAACCGGCAGCTGCAACTGGTGACTTACAGCAGCCGG AGCTACCAGGCCAACACAGCAGACACAGTCCGGTTCTCCACCAAGGGACATGAAGTTGCCTTCACCATCC TCGTAAGACACCCTCCCAACCCCCGGCTGTACCCACCATCATCCCTACCCCAAGGAGCCGAGTACAACAT CAGTGCTCTGGTTACCATTGCCACCAAGACTTTTCTTCGTTATGATCGACTACGGACACTCATTGCCAGC ATCCGACGCTTTTACCCTACGGTCACCATAGTCATCGCTGACGACAGTGACAAACCGGAGCGCATTAGCG ACCCACACGTGGAGCACTACTTCATGCCCTTCGGCAAGGGTTGGTTCGCAGGTCGGAACCTGGCGGTGTC CCAAGTAACCACCAAATACGTGCTGTGGGTGGACGACGACTTTGTCTTCACGGCGCGCACGCGGCTGGAG AAGCTTGTGGACGTGCTCGAGAAGACGCCCCTGGACCTGGTAGGGGGCGCGGTGCGGGAGATCTCGGGCT ACGCTACCACCTACCGGCAGCTGCTGAGTGTGGAGCCGGGCGCCCCAGGCCTTGGGAACTGCTTCCGGCA AAAGCAGGGCTTCCACCACGAGCTCGTTGGCTTCCCAAGTTGTGTGGTCACAGACGGCGTGGTCAACTTT TTCCTGGCGCGCACAGACAAAGTGCGCCAAGTGGGCTTTGACCCACGCCTCAACCGGGTGGCACATCTGG AATTCTTCCTGGATGGACTTGGTTTCCTTCGTGTTGGCTCCTGCTCTGATGTTGTTGTGGATCATGCATC GAAGGTGAAGCTGCCGTGGACAGCAAAGGACCCAGGAGCTGAAACTTATGCCCGTTACCGGTACCCGGGA TCACTGGACCAAAGTCAAGTGGCCAAACATCGCCTACTCTTCTTCAAACACCGGCTACAGTGCATGACCG CCGAGTGACGTCTGATTTGGGCCTTCACACTGTCAGGCTGGGCCTGCCTCCTTGTTCCTTCCAGGATTAT CCACCACCACCTCCATCCCTGTGCACCCCACTGATGAACACCCTGGCTTCCCGATCCTCTCCACCAATCT GATTCCTAACAGGGGCTTGTCCTGGTGACACCCTTCCTTTCTGTGAGCGACCAGAGGCCCGATGGAGCCA TATCCTCTTCCCCCCACAGCCAGTGCCAAGTCCTCCCACAACCCCATTCCTATGGGGCAGGAAATGGGGA GGTTCATTTTCCAAGTGCCAAAGAGCCCAGAAGGACTCTAAGACCCTCAAGTGGAAACACTCTCACCTCC CGAGGTGGGCAGGGAAACTCCCAATTTGCAACCCCAGGGACATGCACCCCACCCCAGCTCTGGATCCAGC ACCGTGTGTACCGGCTCCCACATACCCCCACAGAAAGCACTGTGACTGTAGTTCTGTGGGGCTGGTGAAC ACACGGTGAAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5315443 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5315443 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6869 same embryo
	EMAGE:6867 same embryo
	EMAGE:6868 same embryo
	EMAGE:6870 same embryo
	EMAGE:6866 same embryo
	EurExpress:euxassay_002539 same experiment
	MGI:4823377 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source