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EMAGE:6882

Calml4 calmodulin-like 4 ( MGI:1922850)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6882 EMAGE:6882 EMAGE:6882 EMAGE:6882 EMAGE:6882
"Pseudo-wholemount" of euxassay_002512. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_002512_01 euxassay_002512_02 euxassay_002512_03 euxassay_002512_04
EMAGE:6882 EMAGE:6882 EMAGE:6882 EMAGE:6882 EMAGE:6882
euxassay_002512_05 euxassay_002512_06 euxassay_002512_07 euxassay_002512_08 euxassay_002512_09
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euxassay_002512_10 euxassay_002512_11 euxassay_002512_12 euxassay_002512_13 euxassay_002512_14
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euxassay_002512_15 euxassay_002512_16 euxassay_002512_17 euxassay_002512_18 euxassay_002512_19
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euxassay_002512_20 euxassay_002512_21 euxassay_002512_22 euxassay_002512_23 euxassay_002512_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6882Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6882_wholemount_strong.wlz
6882_wholemount_moderate.wlz
6882_wholemount_weak.wlz
6882_wholemount_possible.wlz
6882_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6882_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
choroid fissure
strong strong
regionalstrong expression: see section 07
lateral ventricle choroid plexus
strong strong
regionalstrong expression: see section 05 06 08 09 10 11 12 13 14 15 16 17 18
4th ventricle lateral recess
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T3034
Entity Detected:Calml4, calmodulin-like 4 ( MGI:1922850)
Sequence:sense strand is shown

>T3034
TGGCCTCGAGCCAGATTCGTCGACTCCCTGAGGATGGCCAAGTTCCTTTCCCAGGACCAAATTAATGAGT
ACAAGGAGTGCTTCTCCCTGTACGACAAGCAGCAGAGAGGGAAGATCAAGGCCACAGATCTTCTGGTGTC
CATGAGGTGCCTAGGGGCCAGCCCCACGCCTGGGGAAGTGCAGCGGCACCTGCAGACTCATGGGATAGAC
AAGAACGGAGAGCTGGACTTCTCCACCTTCCTGACCATCATGCATATGCAAATCAAGCAAGAGGACCCAA
AGAAAGAAATCCTTCTGGCCATGCTGATGGCAGACAAGGAGAAGAAAGGTTACATCATGGCGTCTGAGCT
GCGGTCGAAACTCATGAAACTGGGAGAGAAGCTCACTCACAAGGAAGTGGATGATCTTTTCAAGGAAGCA
GGCATTGAACCAAACGGCCAAGTGAAATATGACACGTTTATCCAGAGGATCACCATTCCTGTGCGAGACT
ACTAAGGGAGCCAAGATCAAGAGAGCAGATGAGAGCCTTCGGGGCCTGAAAACCTAGACTGATTCCTTTT
TGTAAGG
Notes:The probe template was PCR amplified from IMAGE:1545963 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:1545963 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6879 same embryo
 EMAGE:6881 same embryo
 EMAGE:6878 same embryo
 EMAGE:6880 same embryo
 EurExpress:euxassay_002512 same experiment
 MGI:4823597 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS