EMAGE mouse gene expression spatial database

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EMAGE:6907

Grm8 glutamate receptor, metabotropic 8 ( MGI:1351345)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002446. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002446_01	euxassay_002446_02	euxassay_002446_03	euxassay_002446_04

euxassay_002446_05	euxassay_002446_06	euxassay_002446_07	euxassay_002446_08	euxassay_002446_09

euxassay_002446_10	euxassay_002446_11	euxassay_002446_12	euxassay_002446_13	euxassay_002446_14

euxassay_002446_15	euxassay_002446_16	euxassay_002446_17	euxassay_002446_18	euxassay_002446_19

euxassay_002446_20	euxassay_002446_21	euxassay_002446_22	euxassay_002446_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6907_wholemount_strong.wlz

6907_wholemount_moderate.wlz

6907_wholemount_weak.wlz

6907_wholemount_possible.wlz

6907_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6907_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 09 10 weak expression: see section 11 16 17 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 09 10 11 12 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4044
Entity Detected:	Grm8, glutamate receptor, metabotropic 8 ( MGI:1351345)
Sequence:	sense strand is shown >T4044 TATGCCATTAAAACCAGAGGGGTTCCAGAAACGTTCAATGAAGCCAAACCTATTGGATTTACCATGTACA CCACGTGCATCATTTGGTTAGCTTTCATTCCCATCTTTTTTGGTACAGCCCAGTCAGCAGAAAAGATGTA CATCCAGACAACAACACTTACTGTCTCCATGAGTTTAAGTGCTTCAGTGTCTCTGGGAATGCTCTATATG CCCAAAGTTTATATTATAATTTTTCATCCAGAGCAGAACGTTCAAAAACGCAAGAGAAGCTTCAAGGCTG TGGTCACGGCCGCTACCATGCAAAGCAAACTGATCCAAAAGGGAAATGACAGACCAAACGGCGAGGTGAA AAGTGAACTCTGTGAGAGTCTTGAAACCAACAGTAAGTCATCTGTAGACTTTCAGATGGTCAAGAGCGGG AGCACTTCCTAATAGATCTTCTTCTACCAAGACAACATACATCAGCTACAGTAATCATTCAATCTGAACA GGGAGATGGCACCATCTGAAGGAAGGTGCTGTATATTCTTAAATACTGAACATGAGACTGCCANAACGTA CTCTGGGAGATCTCCATAGACTACAATCAATCAGATCAATAGTCTC
Notes:	The probe template was PCR amplified from IMAGE:5688688 using vector specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:5688688 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6905 same embryo
	EMAGE:6910 same embryo
	EMAGE:6909 same embryo
	EMAGE:6906 same embryo
	EMAGE:6908 same embryo
	EurExpress:euxassay_002446 same experiment
	MGI:4825242 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source