EMAGE mouse gene expression spatial database

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EMAGE:6910

Kif3a kinesin family member 3A ( MGI:107689)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002371. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002371_01	euxassay_002371_02	euxassay_002371_03	euxassay_002371_04

euxassay_002371_05	euxassay_002371_06	euxassay_002371_07	euxassay_002371_08	euxassay_002371_09

euxassay_002371_10	euxassay_002371_11	euxassay_002371_12	euxassay_002371_13	euxassay_002371_14

euxassay_002371_15	euxassay_002371_16	euxassay_002371_17	euxassay_002371_18	euxassay_002371_19

euxassay_002371_20	euxassay_002371_21	euxassay_002371_22	euxassay_002371_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

6910_wholemount_strong.wlz

6910_wholemount_moderate.wlz

6910_wholemount_weak.wlz

6910_wholemount_possible.wlz

6910_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:6910_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4046
Entity Detected:	Kif3a, kinesin family member 3A ( MGI:107689)
Sequence:	sense strand is shown >T4046 TGAGCCGCAGTCTCTGGAGCGCTAAGGTGTTGCGGTCGCCTGTGGCCAGCCTCGCCCCCAAACCCGGTGT TGTTGGGCTGCATCCGGAGTGGGGACGTTGGCGGAGCACCGGACGCGGCCACCATGCCGATCAATAAGTC GGAGAAGCCGGAAAGCTGCGATAATGTGAAGGTGGTGGTTAGGTGCCGGCCTCTCAATGAGAGAGAGAAG TCAATGTGCTATAGACAGGCCGTCAGCGTGGATGAAATGAGGGGAACTATCACCGTCCATAAGACCGATT CCTCCAACGAACCTCCAAAGACATTTACTTTCGATACTGTTTTTGGACCAGAAAGTAAACAACTTGATGT GTATAACTTAACTGCAAGACCAATTATTGATTCTGTTCTGGAAGGCTACAATGGGACCATCTTTGCATAT GGACAGACTGGGACAGGCAAGACTTTCACCATGGAAGGCGTTCGAGCAGTACCGGGGCTCAGAGGGGTCA TCCCCAACTCATTTGCTCACATATTTGGTCACATCGCAAAAGCAGAGGGCGACACAAGGTTTTTGGTTCG AGTGTCATACTTGGAGATATATAATGAAGAAGTTCGAGACCTTTTGGGTAAAGATCAGACCCAGAGGTTA GAGGTTAAAGAAAGGCCTGATGTGGGAGTATATATCAAAGACTTATCAGCATATGTAGTCAACAACGCTG ACGACATGGATAGAATCATGACTCTAGGCCACAAAAACCGTTCTGTCGGTGCGACTAATATGAACGAGCA CAGCTCCCGATCCCACGCCATCTTTACAATTACTATAGAGTGCAGTGAGAAAGGCGTGGACGGGAACATG CACGTCAGGATGGGCAAGCTCCACCTTGTGGATCTGGCTGGGTCAGAAAGACAAGCAAAAACTGGAGCCA CAGGACAGCGCCTCAAGGAAGCTACCAAAATCAACCTTTCATTGTCTACCCTGGGCAACGTTATTTCTGC CTTGGTGGATGGAAAAAGCACTCACGTGCCGTATCGGAACTCCAAACTGACCCGTCTCCTGCAGGACTCC TTAGGAGGGAACTCAAAAACCATGATGTGTGCAAATATTGGGCCAGCAGATTATAATTATGATGAAACTA TCAGCACCTTGCGGTATGCCAACCGCGCCAAGAACATTAAAAACAAAGCGAGAATCAATGAGGACCCAAA GGATGCTCTGCTCCGCCAGTTTCAGAAAGAAATCGAAGAGCTGAAGAAAAAGCTTGAGGAAGGGGAAGAA GTGTCAGGCTCTGACATCAGTGGGTCAGAGGAGGATGACGAGGAGGGTGAACTTGGAGAAGATGGAGAAA AAAAGAAAAAGAGAAGGGACCAAGCAGGTAAAAAGAAAGTTTCCCCAGATAAGATGGTGGAAATGCAAGC GAAAATTGACGAGGAGAGAAAAGCACTGGAAACCAAACTTGACATGGAAGAAGAAGAAAGAAACAAGGCT AGAGCTGAACTAGAGAGACGGGAGAAGGACCTTCTGAAAGCCCAACAAGAGCATCAGTCTTTGCTGGAAA AGCTGTCTGCTTTGGAAAAGAAGGTCATTGTCGGCGGCGTGGACCTGCTGGCCAAGGCTGAGGAGCAAGA GAAGCTTCTGGAGGAGTCCAACATGGAGCTGGAGGAGAGGAGGAGACGAGCTGAGCAGCTTCGGAAAGAG CTGGAGGAAAAGGAGCAAGAACGCTTGGACATCGAGGAAAAATATACCAGTCTGCAAGAGGAGGCACAAG GAAAGACCAAGAAATTAAAGAAAGTCTGGACCATGCTGATGGCTGCAAAGTCAGAGATGGCTGATCTCCA GCAGGAGCATCAGAGGGAGATCGAAGGCCTCCTGGAGAACATCCGGCAGCTCAGCCGGGAGCTCCGTCTT CAGATGCTCATCATTGATAACTTCATACCTCAGGATTACCAGGAAATGATTGAAAACTACGTCCACTGGA ATGAAGACATAGGAGAATGGCAGCTAAAATGTGTGGCCTACACAGGAAATAACATGAGGAAACAAACTCC AGTCCCTGACAAGAAGGAGAGAGACCCATTTGAAGTCGACCTTTCCCACGTGTACCTCGCCTATACAGAG GAGAGTCTGCGTCAGTCTCTGATGAAGTTAGAAAGGCCACGGACCTCCAAGGGGAAAGCAAGGCCAAAGA TGGGGCGAAGAAAGCGTTCTGCAAAGCCTGAGACCGTAATTGATTCTTTACTTCAGTAAATGTACAGAAT TAAAGTTAAAATAATAAAAAGAGTGCTGTCCCATGCCTGGAGAGAACTCCTTAATTAAGACACGAGGACA ACTGTATGGTTCACATAATGGAAGCTGTAAAGCATGGAGAGAGACTGGAAATAGCAATTTTCCTAATATC TTTTAGACTCTTTAAGTGTCTGTAACATGTAAGTGCGCCGCAGCTCATGGTCGTTGGGCATGGCGCACTG TGTCTGTCACACTGCTCTGCAGGAAGTAAGGGTTCCGTTGTTGTACGTGAATGTGCGTTTCTTAGCTATG TATTCAACTACCGTGGCTCTAGACAAGCAGAATCTCAGACTAGAACCAAGTCTGTCTTGTTTGCACACAA CATATAGCAGATCACCTTGTCCTGTGTCCTGTGTCCTGTGTCCTGTGCCAGTCTGGGCTGCCCATCTAAG CAGTGACTTGATTCTGTACCTGCCTGACCTGTCTAAATGCAGTCCAACTTTACACTCATCCTGACTGCAT CTAAGTGTGCAATGACTACCTTGATTGATCTCCTGTGGAAAACTATAAAATCTGACAGCTCTGCAGGCTT ACATTCTGAAGATGCAGTTTGTGTGCTCGCCTGTGCACCCTCCACTTCTGTAGCTCTGACTCCACATTCA GCCATTGTTGTTACTAAGTTGGCTCCCCACTTCTCCTGAAGCCAGCATTAGCCACTTACCCTTGCTCACA CACACATTCTAAAACATTCCCTGGACATACAGCTTACAAACACTTCCTTGTCTGAATTTTAAGTTTGATG GAAATAACTATAAAAAATGAGACCAGGGGTTGGGGATTTAGCTCAGTGGTAAAGTGCTTGCCTAGCAAGC CCAAGGCCCTGGGTTCGGTCCTCAGCTCCGGAAAAAAGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5702415 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5702415 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:6905 same embryo
	EMAGE:6909 same embryo
	EMAGE:6907 same embryo
	EMAGE:6906 same embryo
	EMAGE:6908 same embryo
	EurExpress:euxassay_002371 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source