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EMAGE:6962

Mtap1a microtubule-associated protein 1 A ( MGI:1306776)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962
"Pseudo-wholemount" of euxassay_007204. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007204_01 euxassay_007204_02 euxassay_007204_03 euxassay_007204_04
EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962
euxassay_007204_05 euxassay_007204_06 euxassay_007204_07 euxassay_007204_08 euxassay_007204_09
EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962
euxassay_007204_10 euxassay_007204_11 euxassay_007204_12 euxassay_007204_13 euxassay_007204_14
EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962 EMAGE:6962
euxassay_007204_15 euxassay_007204_16 euxassay_007204_17 euxassay_007204_18 euxassay_007204_19
EMAGE:6962 EMAGE:6962
euxassay_007204_20 euxassay_007204_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:6962Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
6962_wholemount_strong.wlz
6962_wholemount_moderate.wlz
6962_wholemount_weak.wlz
6962_wholemount_possible.wlz
6962_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:6962_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4055
Entity Detected:Mtap1a, microtubule-associated protein 1 A ( MGI:1306776)
Sequence:sense strand is shown

>T4055
CGACACACCCCCAGAAAAACCTCCAAGCCAGAGAGGGTGAGGACAGAGTCCAGCGAAGCACTGAAGGCTG
AGAAGCGGAAGCTGATCAAGGATAAAGTGGGCAAGAAGCACCTGAAGGAAAAGATTTCAAAGCTGGAGGA
GAAGAGGGACAAGGAGAAGAAGGAGATCAAGAAGGAAAGGAAGGAACTCAAGAAGGAGGAGGGAAGGAAG
GAGGAGAAAAAGGACGCCAAGAAGGATGAAAAGAGGAAAGATACCAAGCCGGAACTCAAGAAATTCTCTA
AACCAGACCTGAAGCCTTTTACCCCTGAGGTCCGTAAGACCCTCTACAAAGCCAAGGCCCCTGGAAGGCT
CAAGGTGGACAAAGGCCGAGCTGCCCGTGGGGAAAAAGAATTGTCTTCTGAGCCCCGGACACCCCCAGCC
CAGAAGGGGGCTGCACCGCCTCCTGCTGCCAGTGGGCACAGAGAGTTGGCCTTGTCTTCACCAGAGGACC
TCACACAGGACTTTGAGGAGTTGAAGCGTGAGGAGAGAGGTTTGTTGGCTGAACCAAGGGACACGGAACT
GGGTGAGAAACCGCTCCCTGCAGATGCCTCCGAGCAGGGACGCCCAAGCACAGCCATCCAGGTGACCCAA
CCCCCTGCTTCAGTGTTGGAGCAAGAACAGGTAGAAAGGGAGAAAGAG
Notes:The probe template was PCR amplified from IMAGE:6820275 using vector specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:6820275 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:6960 same embryo
 EMAGE:6959 same embryo
 EMAGE:6961 same embryo
 EurExpress:euxassay_007204 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS