EMAGE mouse gene expression spatial database

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EMAGE:7001

Mbnl3 muscleblind-like 3 (Drosophila) ( MGI:2444912)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002568. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002568_01	euxassay_002568_02	euxassay_002568_03	euxassay_002568_04

euxassay_002568_05	euxassay_002568_06	euxassay_002568_07	euxassay_002568_08	euxassay_002568_09

euxassay_002568_10	euxassay_002568_11	euxassay_002568_12	euxassay_002568_13	euxassay_002568_14

euxassay_002568_15	euxassay_002568_16	euxassay_002568_17	euxassay_002568_18	euxassay_002568_19

euxassay_002568_20	euxassay_002568_21	euxassay_002568_22	euxassay_002568_23	euxassay_002568_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7001_wholemount_strong.wlz

7001_wholemount_moderate.wlz

7001_wholemount_weak.wlz

7001_wholemount_possible.wlz

7001_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7001_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5178
Entity Detected:	Mbnl3, muscleblind-like 3 (Drosophila) ( MGI:2444912)
Sequence:	sense strand is shown >T5178 CTGAAGGATCACGTAACTCAGAAAATCTAAAACACATTATGTGTCCAAATCAGTTCTTCTGAGTTACGCG GACGCGTGGGTTTCACGACGCAAGTGCGTCCTACAGGAAGAAAGTGCCCCCAGTCGGAGCGCGAGCAGGA GCGCGACTTTTTGGCGCTCTTTGCGAGCGAGCCGCAAGGAGGCGGAAGACGGTCCCGGGCCGGGGCGCGG GAATCGGGGCAGCGAGCGCCGCACGGGGGAGTTCCTGCGCGTGGCGTCCTCGCAGCGAGACGCCGCTGGA GTCGCTCACTCGGAGAGATTCCTTGAACCATCTGCAGTCATAATATTCTCTGAAGAGGGTGCACTTGATT GCCAATTTGCTCTCAGTATGACACCTGTCAATGTAGCTCTAATCCGTGATACCAAGTGGCTGACTTTAGA AGTCTGTAGAGAATTTCAGAGAGGAACTTGCTCTCGAGCTGATGCAGAGTGCAGGTTTGCCCATCCGCCA AGAGTTTGCCATGTGGAAAATGGCCGAGTGGTGGCCTGTTTTGATTCACTAAAGGGTCGGTGCACTCGTG AGAACTGCAAGTACCTCCACCCTCCACCGCACTTAAAGTCGCAGCTAGAAGTTAATGGGAGAAACAATCT GATTCAACAGAAGACTGCCGCAGCCATGTTCGCCCAGCACATGCAACTCATGCTGCAGAACGCTCAGATG TCATCTCTTGCGTCTTTTCCTATGAATCCATCACTTGCAGCTAATCCTGCCATGGCTTTCAATCCTTACA TGACTCATCCTGGCATGGGCCTGGTTCCTGCTGAGCTTTTACCAAATGGTCCGGTTCTGATTTCTGGAAA CCCTCCTCTTGCACTGCCAGGAGTTCCTGGTCCAAAGCCAATTCGTACAGATAGACTGGAGGTTTGCCGT GAATTTCAGCGTGGAAATTGTACCCGTGGGGAGAGCGAGTGCCGCTATGCTCACCCTACGGATGTTTCCA TGATTGAAGTCACTGATAATTCTGTGACAATCTGCATGGATTACATTAAAGGCCGATGCTCCCGGGAGAA ATGCAAGTACTTTCATCCTCCTCCCCACTTGCAGGCCAAACTCAGGGCAGCTCATCACCAGATGAACCAT TCTGCTGCCAATGCAATGGCCCTGCCGCATGGTGCACTTCAACTGATACCAAAGAGGTCAGCCCTTGACA AGGCCAATGGTGCCACTCCAGTCTTTAACCCCAGTGTTTTCCACTGCCAACAGGCTCTGGCTAACATGCA GATTCCTCAGCAGGCTTTTATCCCAACAGTGCCCATGATGCACGGTGCTACACCTTCCACTGTGTCTACA GCAACACCACCTGCCAGCAACGTTCCCTACGTTCCAACAACTACAGGCAACCAGTTGAAATATTGAGCAG CAGAGTTACAGAGTATCAGAATCTCTCAACAAGAAACTCCGTGTGGCCTTTCTATATGTATTCTCGTATG TCTTCTTGTACCAACACGACAATAAGCATGGTGCAGTCAATATACTAAAGCGCATATACCTGTTGACAAA TTCAAATTTTAAAAATCTGTGGAGATGTTAAAGCAAATAGAAAATTAACCAGTATGTGTTACCTTATACG GATTCATTGTATATGAATTAGCATACAATATACAACCATACAGGTTTGTCATGTATATGAATTATCAGAT CCATATTACATGAATTTTCCATATGATATGAATTACCATATTGAATATAACTGTAAAATGTTGTGACTGC TTTCCAGTAATGGTTTATAATAAATGAACTTCCACAGTGTACTGTAGGCTTACTGTATACTCTTGGTGGA TAAATTCTGTTTTGGAAGTGTTACCTTACTGTTTTGTTTACAAGATAGTCTATAGGATTGATGTAGAATG TAACTGATATTTCCCACACCATTTTCCTCCATTGGTATATTGTATTAAATTGGGTTCTGCTTAAAAAAAA AAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5348959 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5348959 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7003 same embryo
	EMAGE:7002 same embryo
	EMAGE:7004 same embryo
	EMAGE:7005 same embryo
	EMAGE:7000 same embryo
	EurExpress:euxassay_002568 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source