EMAGE mouse gene expression spatial database

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EMAGE:7002

Ddx19b DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b ( MGI:2148251)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002562. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002562_01	euxassay_002562_02	euxassay_002562_03	euxassay_002562_04

euxassay_002562_05	euxassay_002562_06	euxassay_002562_07	euxassay_002562_08	euxassay_002562_09

euxassay_002562_10	euxassay_002562_11	euxassay_002562_12	euxassay_002562_13	euxassay_002562_14

euxassay_002562_15	euxassay_002562_16	euxassay_002562_17	euxassay_002562_18	euxassay_002562_19

euxassay_002562_20	euxassay_002562_21	euxassay_002562_22	euxassay_002562_23	euxassay_002562_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7002_wholemount_strong.wlz

7002_wholemount_moderate.wlz

7002_wholemount_weak.wlz

7002_wholemount_possible.wlz

7002_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7002_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

orbito-sphenoid

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 18 20 21 22 23 24

viscerocranium

moderate

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5133
Entity Detected:	Ddx19b, DEAD (Asp-Glu-Ala-Asp) box polypeptide 19b ( MGI:2148251)
Sequence:	sense strand is shown >T5133 CCACGCGTCCGCCTGAGTCCGCGGTCGCACCGCCCAAGCGGGCAAGCCTGCCAGCGCTGCCCCGGCACCG CGGGCCATGGCTACGGATTCTTGGGCCCTAGCAGTAGACGAACAGGAAGCCGCAGCTGAGTCTTTGAGCA ACTTGCATCTTAAGGAGGAGAAAATCAAACCAGATGCCAATGGTGCTGTTGTTAAGACCAATGCAAACAC AGAGAAGACAGAGGAAGATGAGAAAGAGGACAGAGCTGCTCAGTCTTTACTCAACAAGCTGATCAGAAGC AATCTTGTGGATAACACCAACCAAGTGGAAGTCCTGCAGAGGGACCCAAGCTCCCCGCTCTACTCAGTGA AGTCCTTCGAAGAGCTTCGCCTGAAACCACAGCTTCTCCAGGGAGTCTATGCTATGGGTTTCAACCGCCC TTCCAAAATACAGGAAAATGCATTGCCTTTGATGCTTGCTGAACCCCCACAGAACTTAATTGCCCAGTCT CAGTCTGGTACGGGTAAAACAGCTGCCTTTGTTTTGGCTGTGCTCAGCCAAGTAGAGCCTGCAAACAAGT TTGCCCAGTGTCTCTGCCTCTCTCCAACCTATGAGCTTGCTCTTCAAACAGGAAAAGTGATTGAGCAGAT GGGCAAGTTTTACCCTGAACTGAAGCTAGCTTATGCTGTTCGAGGCAACAAATTGGAGAGGGGTCAGAAG GTGAGTGAGCAGATTGTCATTGGCACCCCCGGGACCGTCTTGGACTGGTGCTCCAAGCTGAAGTTCATTG ACCCCAAGAAGATCAAGGTGTTTGTTCTGGACGAGGCTGATGTGATGATAGCGACTCAGGGCCACCAAGA CCAGAGCATCCGCATCCAGAGGATGCTGCCCAGAAACTGCCAGATGCTGCTTTTCTCGGCCACCTTTGAA GACTCGGTGTGGAAGTTTGCCCAGAAGGTGGTCCCAGACCCCAACATCATCAAGCTGAAGCGTGAGGAGG AGACTCTGGACACCATCAAACAGTACTACGTCCTCTGCAATAACAGAGAGGAGAAGTTCCAGGCCCTGTG CAATCTGTATGGGGCCATCACCATCGCCCAAGCCATGATCTTCTGCCATACCCGCAAAACAGCTAGCTGG CTGGCAGCAGAGCTCTCAAAAGAGGGCCACCAGGTGGCCCTGCTGAGTGGAGAGATGATGGTGGAGCAGA GGGCTGCGGTGATCGAGCGCTTCCGAGAAGGCAAAGAGAAGGTTCTGGTGACCACCAACGTGTGTGCCCG TGGTATCGATGTTGAACAGGTGTCTGTCGTCATCAATTTTGACCTCCCTGTGGACAAGGACGGGAACCCA GACAATGAGACCTACCTGCACCGCATTGGGCGCACTGGCCGCTTTGGCAAGAGGGGTCTGGCGGTCAACA TGGTGGACAGCAAGCACAGCATGAATATCCTCAACAGGATCCAGGAGCATTTTAATAAGAAGATAGAAAG ACTGGACACAGATGATTTGGACGAGATTGAGAAAATCGCCAACTGAGGCGCTCGCTTCTCCATGGGCTGG CGCCACCCTCAGCGCTCCCTGCCTGGGAGTCTAGTGCTTTCATGGCATAGGCCTGACGGGCCCCTCAGAG ACCACGGGCTGAGTAGAGACTACCTACCTCATTCGGAATTACGTTTGGACTTGACAAATTTGTGCAAATG ATGAGGGATGGTAGAAATGTGTTTACACAACTTTGGAAGATTAGGCATGAATACACAGAAATTTACCTTT TCAAGTCCCATCTTTTTTTTTTGGACAGTATTTTCCCATCACACTGATCTAGATAATATGCCTAGGATTC CTCATGACAAACCTTAGCTGTTTGTGACTTGGAGTTTGCAGGCCAGCCTCGTGGAAGTGACAGCACAGAA CAGAGAGCACGTAGAGGCAGAGTCCTCAGGGAGGAGGAGGATATAAGCCAGTCGTGTGTCAGGAAGATGG CACAAGGCACTGGAGTTGGCCTGTGGACTGCTGCTTAGGACTTGGTTGGTGTGTAATGGCTAAGCTGTGT GTTACCTGACCCCTGCTGACATTCTTTCCAGGAAAAGATTTGCCATGTTGGACCAAAATGCCTTTGGGGC CTCTCCTTAAGCTTTGTGGCTCAGGAACATGGGCAAATCTAGTTATTGGTGAGTTCTTGGCTCATACTTC AGCAAGACCTGTGGAGAAGTTGTCCTGGAGGATTAGTGGTGCCTCTGGCTTCCGGTAACACCTGGATCAT GGGTACTTGAACCATAGTTTTTACATCTGTAACATCACTTGGACTGTTAGTAGTCCCAGGCAAATAACAG GGTACTGAGGTTTGGGTTCTAAGTGAGTACTGTAGCTTAAAGTTTCTGATGGGCTGGCAGCCTTGATCAG AGCAGGCTAGGCATGGTCAGCAGTTGCCAAAATTCCTTGCTTTGTTTGCAGTTCCTGAAAGATTAGTCCT GTCAGAGCTGGGGAGTGACTGCAGATATTGGGCAACGTGGCTCTAAAGGGATTCTGATGGTCTGTGTTCA CAGTGAGTGTGATGAGGGACAGGACGGACCACAGCCGTTTGTCTGTCCACAATGAGTAATCAACAAGTAC TTGCTGCTTTCTCCCTTAGCATCCATCATTAGGACAGTAAAAGCACACAATAAACAGAGAACTTCTCACA CTGGCTAGGCCATAGATACTACAGAGTGACAGCAATGTGGAGGGATTGAGAAGCATCCTCTTCAGTTCTT TGACTTGGACATGGGGTGAGTCTTCTCTCTAGGGCTTGTTTTGTTAGTTTTGTTGCATGTATGTGTTGTG CTGGGACTCAAAGCCTTGCGTTCTGCTACCTAAGCACGGTTACTCTGAACTATTATTTCCAGCCATGATA GAGGCTTGTTGTGGTGAGAAGTATAGACCTGTGTTCCCATATCTTAAGGCCAGTTCCGTCCCCTGGGAAA GGAGAGTGCCATGGCTACCACCTGGTGGTGGTTCAATGTCACAGAGAAATCAACAGGTGCTTGTTCTCCA CTCTCCAGTGGGTATTTGAATAGAATTCTCTGTGGGCTCCTGGCCAACTTTGATCTATTTTAGGAGTCTT AAAAGGAAAGAATAAACTTTTTCTCCCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5342859 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5342859 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7003 same embryo
	EMAGE:7004 same embryo
	EMAGE:7001 same embryo
	EMAGE:7005 same embryo
	EMAGE:7000 same embryo
	EurExpress:euxassay_002562 same experiment
	MGI:4824269 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source