EMAGE mouse gene expression spatial database

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EMAGE:7004

Lmo2 LIM domain only 2 ( MGI:102811)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002567. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002567_01	euxassay_002567_02	euxassay_002567_03	euxassay_002567_04

euxassay_002567_05	euxassay_002567_06	euxassay_002567_07	euxassay_002567_08	euxassay_002567_09

euxassay_002567_10	euxassay_002567_11	euxassay_002567_12	euxassay_002567_13	euxassay_002567_14

euxassay_002567_15	euxassay_002567_16	euxassay_002567_17	euxassay_002567_18	euxassay_002567_19

euxassay_002567_20	euxassay_002567_21	euxassay_002567_22	euxassay_002567_23	euxassay_002567_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7004_wholemount_strong.wlz

7004_wholemount_moderate.wlz

7004_wholemount_weak.wlz

7004_wholemount_possible.wlz

7004_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7004_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

not examined

regional

not examined expression: see section 01 02 03 22 23 24

neural retina

strong

regional

strong expression: see section 01 02 03 22 23 24

liver lobe

moderate

regional

moderate expression: see section 01 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 weak expression: see section 02 03 04 16 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5179
Entity Detected:	Lmo2, LIM domain only 2 ( MGI:102811)
Sequence:	sense strand is shown >T5179 GCCCCCTCACTGCCCCCGGCGCCCCGACCCCGGCGAAGGTGCGAGGGGCTCGGGGCGACCGGGCGGAGCC GGCTACTGCGCGCGATCGGGACCGGGCGGCGGCGGCGGCGGCTGTGGTGACGGCAGCGGGACCGGGATGG AAGGTTAAGTCCTGAGCAGCGGCAGCTGTCGCAGCCACCGGAGGGAGGCCGGGCTGCTCCCCGGCCCCCT GCCTTGGCCGCCGCGCGCCGGGCAGCTGGATGATTCGCTCTCTCTCTTTGGCGTTTGAAGGGAGCGCGGT GACTGTCCTTGAGCGTGGAGGGGCGAGCTCGCCAGCGGAGCGACCGAGCAAGAGGAGGCGCAGGAGCGGC GGCGCCCGAGCACCCGAGGGGGTCCGAGTCCAGGCAGCTAGCCAGCCACGCGCCACAAAGGGAGCGCCCC CGCCGCCCGGCACGCCGCCCCCCTCCCCAATGTCCTCGGCCATCGAAAGGAAGAGCCTGGACCCGTCTGA GGAACCCGTGGATGAGGTGCTGCAGATACCCCCATCCCTGCTGACATGTGGTGGCTGCCAGCAGAACATA GGGGACCGCTACTTCCTGAAAGCCATCGACCAGTACTGGCATGAGGATTGCCTCAGCTGTGACCTCTGTG GGTGTCGGCTGGGAGAGGTGGGGAGGCGCCTCTACTACAAGCTGGGACGGAAATTGTGCAGGAGAGACTA TCTCAGGCTTTTTGGTCAGGATGGTCTCTGTGCATCCTGTGACAAGCGGATCCGTGCCTATGAGATGACG ATGCGGGTGAAAGACAAAGTGTATCACCTGGAGTGTTTCAAATGCGCCGCCTGTCAGAAGCATTTCTGTG TAGGTGACAGATACCTTCTCATCAACTCCGACATAGTGTGTGAACAAGACATCTACGAGTGGACCAAGAT CAATGGGATCATCTAGGCCAGAGTCCCAGGCACCTGTGGGGAGGTGTTCAGTGAAGACGCCGTCTCGGTG TGGTCTTCACTCTTAGGCTCTTTGGGAACCTGAGGGTGGGTGAGGCATTTCTTAGGAGATTATGGACTCT TCCTGGGCACTAACGACATAGCAATCACATGACATAATGCAAAGGCTGAGACTTCAGTGACAAAAAGGAC CAGCCCTTAGAGAGAATTTATGGTCACAGGCTAGCCATAGTAACTGACAAGATTAGTGGAGGAAATATTT GGGGACCAGCATGCACTGTGCCATCCTGATAGGCTTCCCCAGGTAACTATCGATTAGATGTGACCTTGGT GTTCATGGACTAGAGTTGTGCAATTAATTTTTTCCTGGCTTTGGTAACTACCCTGTTCTAGTCACAGTCC TTCACACTAGGAAAGGAAAGAGAGAAGAAATAGTCACTCTTTTTTTCTTTCTTTCTTTTTTCCTTGGTCA CCTTCCCGTGGCCTTAAGCTCTGGAGCCAAGGGAAACTGCATGGAGGTACATTTCAGTTGGGAATGAAAT CCACATCTTTCAACCAGACAAATATTTATAGCGATGTTGATGAATCACTGTTTTTAGACACCTTCATTTT GAGGTGAGGCGCCCCACAGATTGTTTCTATACAAATGTAAATCTTAAAAAAAAAAGGTCAAAGTTGTTCA ACTATTTTATTATCTTAGATTATATCAAAGTATTTGTTGTGTGTCATTAAAAAAAAAACTCGATTCTGAA GACTTAATAAAAATGACAAGCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5349568 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5349568 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7003 same embryo
	EMAGE:7002 same embryo
	EMAGE:7001 same embryo
	EMAGE:7005 same embryo
	EMAGE:7000 same embryo
	EurExpress:euxassay_002567 same experiment
	MGI:4825948 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source