EMAGE mouse gene expression spatial database

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EMAGE:7049

Fkbp5 FK506 binding protein 5 ( MGI:104670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002327. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002327_01	euxassay_002327_02	euxassay_002327_03	euxassay_002327_04

euxassay_002327_05	euxassay_002327_06	euxassay_002327_07	euxassay_002327_08	euxassay_002327_09

euxassay_002327_10	euxassay_002327_11	euxassay_002327_12	euxassay_002327_13	euxassay_002327_14

euxassay_002327_15	euxassay_002327_16	euxassay_002327_17	euxassay_002327_18	euxassay_002327_19

euxassay_002327_20	euxassay_002327_21	euxassay_002327_22	euxassay_002327_23	euxassay_002327_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7049_wholemount_strong.wlz

7049_wholemount_moderate.wlz

7049_wholemount_weak.wlz

7049_wholemount_possible.wlz

7049_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7049_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thymus primordium

strong

homogeneous

strong expression: see section 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3407
Entity Detected:	Fkbp5, FK506 binding protein 5 ( MGI:104670)
Sequence:	sense strand is shown >T3407 GAGTTTTCATTTTTACTTTTTAGCTTGGGTTTTGAAAGGCAAACTCTAGGAGGCTTAAAACGGGCAGTTA CTCTTAAGGCGAGGTAGATACCTCATCCTGCACCGCAGCCTCTGTATTCTGGTGGGAGCTGTAAGGGTTG CGGGCTTCCGGTGTCCATGCATCAAGCCAAAGTCTCCAAAACTTGGGCATTGAATTAGGGCAAATCTTAA ATAGTTTTGGGGTTGGATTTTAGTCCTACAGGTGATGATTGAACGTGGCTGGCTTCTCCACAGCCTAAGG AACCCCCATGGGGACACACCCACCACCCAATGACCCATGCACCTCTGCCTGGACAGCTCACTTCCAGCCC TAAGGACCACTGTGCTCAAGATGTGTGGCTGTAGTAAGACAGTCTCATGTCAGGCATTAAGTCAAGGCCT TCCTTCACACCTGCGGGACAGCGCTTCTGAGGCTCCAGTTCTGCTCTGCTCGAAGCAGGAGGGATGGGTT GTGAAGGAGTCTGGGATGTGACTGGGACCCTCGCATCTCACACTCGGCACTCTGCCTCTTTCCATCC
Notes:	The probe template was PCR amplified from IMAGE:3025604 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3025604 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7050 same embryo
	EMAGE:7053 same embryo
	EMAGE:7051 same embryo
	EMAGE:7052 same embryo
	EMAGE:7048 same embryo
	EurExpress:euxassay_002327 same experiment
	MGI:4824872 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source