EMAGE mouse gene expression spatial database

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EMAGE:7052

Myo5a myosin VA ( MGI:105976)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_002330. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_002330_01	euxassay_002330_02	euxassay_002330_03	euxassay_002330_04

euxassay_002330_05	euxassay_002330_06	euxassay_002330_07	euxassay_002330_08	euxassay_002330_09

euxassay_002330_10	euxassay_002330_11	euxassay_002330_12	euxassay_002330_13	euxassay_002330_14

euxassay_002330_15	euxassay_002330_16	euxassay_002330_17	euxassay_002330_18	euxassay_002330_19

euxassay_002330_20	euxassay_002330_21	euxassay_002330_22	euxassay_002330_23	euxassay_002330_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

7052_wholemount_strong.wlz

7052_wholemount_moderate.wlz

7052_wholemount_weak.wlz

7052_wholemount_possible.wlz

7052_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:7052_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

homogeneous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 moderate expression: see section 21 weak expression: see section 22

facial vii ganglion

strong

regional

strong expression: see section 05 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 08 18 19

trigeminal v ganglion

strong

regional

strong expression: see section 04 05 06 07 08 09 16 17 18 19 20 weak expression: see section 21 22

vagus x ganglion

strong

regional

strong expression: see section 08 17

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19

spinal cord

strong

homogeneous

strong expression: see section 10 11 12 13 14 15 16 17

cervico-thoracic ganglion

weak

regional

weak expression: see section 09 15 16

cervical ganglion

weak

regional

weak expression: see section 09 16

thoracic ganglion

weak

regional

weak expression: see section 13 14 15

dorsal root ganglion

strong

regional

strong expression: see section 08 09 10 11 12 13 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T3408
Entity Detected:	Myo5a, myosin VA ( MGI:105976)
Sequence:	sense strand is shown >T3408 TGGCCTCGAGCCAGATTCGGCAAGAGGTCGATTGACCAACCTGGAGGACACCCCTCGTACTGTTACCTTT AAACAACATTTATTCTTTATCAGTTGGAAACCTGAGTTTACATACAGCTGAAAGGTGGCAGGGAGGAGTG AAAGGAAGGATAAAGGGGTGTGTCTTCAGCTGACTGCTTTTAGTTTAAATCTTTAGTGCTGCATTTAAGT GGCATACAAAATATAATCCCATATGTATGAACTGTTGTGAACAAGGCACAAAGAACTGAAGAAAATCACT GAATACATATCATAGCAGCTTTGGAGCCAATCATGTTGATATTGTCAAACTGGATCACAAAAATAAACTG GCAACATTAAAGTAACTGGCCAAGTAACTTCCTTCTCTGTGTCCCTACGGCATAGCGATGTGAAAGCTGC TGTTTGCTAGGCCGTGTAGATGCCCGTTTGCACTGTAGCTTACTGAGCATCTTAAGTGGCTGCTGTGTGC TGTCTCCTGACCTAAGTGAAAGTCTTACTAGGACTATAAACTACGCTTCTC
Notes:	The probe template was PCR amplified from IMAGE:3025627 using vector specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:3025627 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:7050 same embryo
	EMAGE:7053 same embryo
	EMAGE:7049 same embryo
	EMAGE:7051 same embryo
	EMAGE:7048 same embryo
	EurExpress:euxassay_002330 same experiment
	MGI:4826564 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source